| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
I examined the immunological influence of mineral oil by using mouse. We injected pristine, squalene and laxative (mixture of the mineral oil) to mouse peritoneal cavity. And I evaluated the effect to T cell, Macrophage cell and B cell of mineral oil. We extracted single cells from peritoneal cavity and spleen. We separated the extracted cells to T cells, Macrophage cells and B cells. We evaluated T cell surface receptors (CD28, ICOS, CD40L, PD1). Expression of all surface receptors were increased. Macrophage cell has ICOS-L, CD40, PD-L1, PD-L2 surface receptors. The expressions of all surface receptors were decreased. On the other hand, we evaluated the effect to B cell. B cell has ICOS-L, CD40, PD-L1 surface receptors too. The expression of all surface receptors were also decreased. Pristane, squalene and laxative were same effect to T cell, Macrophage cell and B cell. From these results, mineral oil could stimulate T cell but suppress Macrophage cell and B cell.
|
