| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Outline of Final Research Achievements |
Hyper-IgE syndrome is characterized by the extremely high serum IgE levels, severe atopic dermatitis, and recurrent staphylococcal infections to the skin and lung. This disorder is caused by a substitution of a single nucleotide of a single allele of STAT3 gene. To treat this disorder with genome editing, we need to establish a CRISPER/Cas9, which could discriminate a difference of the single nucleotide. To this end, we established a reporter assay, specifically detect double strand break by CRISPER/Cas9. With this model system, we successfully identified a CRISPR/Cas9, which specifically cleave mutant STAT3 allele, but not cleave wild-type STAT3 allele.
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