Imaging of blood vessels in the normal mouse brain after X-ray irradiation

• Project/Area Number: 15K15448
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Gunma University
• Principal Investigator: KURACHI Masashi 群馬大学, 大学院医学系研究科, 助教 (20271546)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 血管内皮細胞 / 医学放射線生物学 / 神経科学 / 放射線 / 血管新生

Outline of Final Research Achievements

In the normal mouse brain tissue after X-ray irradiation, acute increase of vascular permeability was induced. The amount of vascular endothelial cell growth factor VEGF in the brain decreased. In the X-ray irradiated cerebral cortex, the activated microglia remarkably increased, and the expression of the tight junction protein, claudin-5, which is expressed specifically in vascular endothelial cells, was decreased. X-ray irradiation on Flk1-GFP/Flt1-tdsRed BAC transgenic mice increased the proportion of GFP-positive vascular endothelial cells in brain microvessels. The cerebrovascular images in Tg mouse brain reflecting the expression levels of Flt1 and Flk1 in vascular endothelial cells were obtained using two-photon microscopy.

Research Products

• [Journal Article] Extracellular vesicles from vascular endothelial cells promote survival, proliferation and motility of oligodendrocyte precursor cells. (2016)
  • Author(s): Kurachi, M., Mikuni, M., Ishizaki, Y.
  • Journal Title: PLOS ONE Volume: 11 Issue: 7 Pages: e0159158-e0159158
  • DOI: 10.1371/journal.pone.0159158
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Gene expression alterations in the medial prefrontal cortex and blood cells in a mouse model of depression during menopause. (2016)
  • Author(s): Miyata S, Kurachi M, Sakurai N, Yanagawa Y, Ishizaki Y, Mikuni M, Fukuda M
  • Journal Title: Heliyon Volume: 2 Issue: 12 Pages: e00222-e00222
  • DOI: 10.1016/j.heliyon.2016.e00222
  • Peer Reviewed / Open Access
• [Journal Article] Blood transcriptomic markers in patients with late-onset major depressive disorder (2016)
  • Author(s): Miyata S, Kurachi M, Okano Y, Sakurai N, Kobayashi A, Harada K, Yamagata H, Matsuo K, Takahashi K, Narita K, Fukuda M, Ishizaki Y, Mikuni M
  • Journal Title: PLoS One Volume: 11 Issue: 2 Pages: e0150262-e0150262
  • DOI: 10.1371/journal.pone.0150262
  • Peer Reviewed / Open Access
• [Journal Article] Transplanted microvascular endothelial cells promote oligodendrocyte precursor cell survival in ischemic demyelinating lesions. (2015)
  • Author(s): K Iijima, M Kurachi, K Shibasaki, M Naruse, S Puentes, H Imai, Y Yoshimoto, M Mikuni, Y Ishizaki.
  • Journal Title: J Neurochem. Volume: 135 Issue: 3 Pages: 539-50
  • DOI: 10.1111/jnc.13262
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] X-ray induced disruption of the blood-brain barrier with the localized change in Claudin5 and the activation of microglia in a normal mouse brain (2017)
  • Author(s): Yukihiko Sejimo, Yukari Yoshida, Akihisa Takahashi, Masashi Kurachi, Yasuki Ishizaki
  • Organizer: The 7th Conference of International Society of Radiation Neurobiology
  • Place of Presentation: 越後湯沢ホテル双葉（新潟県南魚沼郡湯沢町）
  • Year and Date: 2017-02-09
  • Int'l Joint Research
• [Presentation] Extracellular vesicles from vascular endothelial cells promote survival, proliferation and motility of oligodendrocyte precursor cells (2017)
  • Author(s): Masashi Kurachi, Sho Osawa, Hanako Yamamoto, Yuhei Yoshimoto, Masahiko Mikuni, Yasuki Ishizaki
  • Organizer: The 7th Conference of International Society of Radiation Neurobiology
  • Place of Presentation: 越後湯沢ホテル双葉（新潟県南魚沼郡湯沢町）
  • Year and Date: 2017-02-09
  • Int'l Joint Research
• [Presentation] 血管内皮細胞由来・細胞外小胞はオリゴデンドロサイト前駆細胞の生存・増殖・運動脳を促進する (2016)
  • Author(s): 石崎泰樹, 倉知 正, 山本華子, 大澤 祥, 好本裕平, 三國雅彦
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台国際センター/東北大学川内北キャンパス（宮城県仙台市）
  • Year and Date: 2016-09-26
• [Presentation] マウス神経幹細胞に対する生存因子としてのBMP4シグナル経路の解析 (2016)
  • Author(s): 山本華子, 倉知 正, 成瀬雅衣, 柴崎貢志, 石崎泰樹
  • Organizer: 第38回日本生物学的精神医学会・第59回日本神経化学会大会合同年会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2016-09-10
• [Presentation] Extracellular vesicles from vascular endothelial cells affect the behavior of oligodendrocyte precursor cells (2016)
  • Author(s): 倉知 正, 山本華子, 大澤 祥, 好本裕平, 三國雅彦, 石崎泰樹
  • Organizer: 第38回日本生物学的精神医学会・第59回日本神経化学会大会合同年会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2016-09-09
• [Presentation] BMP4がマウス神経幹細胞の生存を促進する信号伝達系の解析 (2016)
  • Author(s): 石崎泰樹, 山本華子, 倉知 正, 成瀬 雅衣, 柴崎 貢志
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-07-20
• [Presentation] Transplantation of brain microvascular endothelial cells stimulates remyelination in the white matter infarct. (2016)
  • Author(s): Yasuki Ishizaki, Keiya Iijima, Masashi Kurachi, Koji Shibasaki, Masae Naruse, Sandra Puentes, Hideaki Imai, Yuhei Yoshimoto, Masahiko Mikuni
  • Organizer: The 10th FENS Forum of Neuroscience
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2016-07-02
  • Int'l Joint Research
• [Presentation] 正常脳組織における放射線急性障害の微視的解析 (2016)
  • Author(s): 瀬下幸彦, 吉田由香里, 高橋昭久, 倉知 正, 石崎泰樹
  • Organizer: 第６回国際放射線神経生物学会
  • Place of Presentation: 長崎大学良順会館（長崎県長崎市）
  • Year and Date: 2016-02-12
• [Presentation] 脳微小血管内皮細胞移植による虚血性脱髄巣における再髄鞘化の促進機構の解析 (2015)
  • Author(s): 石崎泰樹、飯島圭哉、倉知正、柴崎貢志、成瀬雅衣、Sandra Puentes、今井英明、好本裕平、三國雅彦
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会合同年会
  • Place of Presentation: 神戸国際会議場、神戸国際展示場（兵庫県神戸市）
  • Year and Date: 2015-12-02
• [Presentation] A role for BMP4 signaling pathway in mouse neural stem cell survival (2015)
  • Author(s): 山本華子， 倉知正， 成瀬雅衣， 柴崎貢志， 石崎泰樹
  • Organizer: 第58回日本神経化学会大会
  • Place of Presentation: 大宮ソニックシティ（埼玉県さいたま市）
  • Year and Date: 2015-09-12
• [Presentation] Effect of exosomes derived from vascular endothelial cells on OPC survival, proliferation and motility (2015)
  • Author(s): 倉知正、三國雅彦、石崎泰樹
  • Organizer: 第58回日本神経化学会大会
  • Place of Presentation: 大宮ソニックシティ（埼玉県さいたま市）
  • Year and Date: 2015-09-12
• [Presentation] Brain microvascular endothelial cells promote survival of oligodendrocyte precursor cells (2015)
  • Author(s): 飯島圭哉、倉知正、柴崎貢志、成瀬雅衣、好本裕平、三國雅彦、石崎泰樹
  • Organizer: 第58回日本神経化学会大会
  • Place of Presentation: 大宮ソニックシティ（埼玉県さいたま市）
  • Year and Date: 2015-09-12
• [Presentation] 血管内皮細胞がオリゴデンドロサイト前駆細胞に及ぼす効果の解析 (2015)
  • Author(s): 石崎泰樹、倉知正、飯島圭哉、好本裕平、三國雅彦
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場、神戸国際展示場（兵庫県神戸市）
  • Year and Date: 2015-07-30
• [Presentation] Extracellular vesicles from brain microvascular endothelial cell cultures promote survival, proliferation, and motility of oligodendrocyte precursor cells. (2015)
  • Author(s): Ishizaki, Y., Kurachi, M.
  • Organizer: European Glial Meeting 2015
  • Place of Presentation: Bilbao, Spain
  • Year and Date: 2015-07-15
  • Int'l Joint Research