Investigation of plasma membrane repair in X-ray and C-ion resistant cancer cells
Project/Area Number |
15K15467
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Radiation science
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Research Institution | Japanese Foundation for Cancer Research (2016) National Institute of Radiological Sciences (2015) |
Principal Investigator |
Katsutoshi Sato 公益財団法人がん研究会, 有明病院 遺伝子診療部, 研究員 (20589650)
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Co-Investigator(Kenkyū-buntansha) |
下川 卓志 国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 放射線障害治療研究部, 主任研究員(定常) (20608137)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | 粒子線治療 / 炭素イオン線抵抗性がん細胞株 / 細胞膜修復 |
Outline of Final Research Achievements |
X-ray and carbon ion beam (C-ion) resistant cancer cell X60 abundantly contained lysosomes, ATP, mitochondria, and reactive oxygen species. In addition, mTOR signaling, which is associated with regulation of energy production, was promoted in X60 cells. Inhibition of the mTOR signaling significantly decreased the X-ray and C-ion resistance in X60 cells. Therefore our results showed that mTOR signaling contributes the X-ray and C-ion resistance in cancer cells. Futhermore, the X60 cells were significantly resistant to plasma membrane damage which is induced by Streptolysin O treatment. Our results indicated that the X60 cells have a lot of Flottilin-1 domain on plasma membrane, which closely associated with lipid raft formation and endocytosis. Given these results, regulation of plasma membrane including lipid raft formation and endocytosis might also contribute to X-ray and C-ion resistance in cancer cells.
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Report
(3 results)
Research Products
(7 results)
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[Journal Article] MicroRNA miR-374, a potential radiosensitizer for carbon ion beam radiotherapy.2016
Author(s)
S. J. Baek, K. Sato, N. Nishida, J. Kosski, R. Azura, K. Kawamoto, M. Konno, K. Hayashi, T. Satoh, Y. Doki, M. Mori, H. Ishii, K. Ogawa.
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Journal Title
Oncology Reports
Volume: 36
Pages: 2946-2950
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Microarray Analysis of Radioresistant Mouse Squamous Cell Carcinoma: Comparison of X-ray Resistance and Carbon-ion Beam Resistance2015
Author(s)
S. Baek, H. Ishii, K. Sato, K. Tamari, K. Hayashi, N. Nishida, M. Konno, J. Koseki, K. Kawamoto, M. Mori, K. Ogawa,
Organizer
15th International Congress of Radiation Research
Place of Presentation
京都
Year and Date
2015-05-25 – 2015-05-29
Related Report
Int'l Joint Research