Transplantation of retinal ganglion cells generated from iPS and ES cells
| Project/Area Number |
15K15640
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | National Center for Child Health and Development |
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Principal Investigator |
Azuma Noriyuki 国立研究開発法人国立成育医療研究センター, 感覚器・形態外科部, 医長 (10159395)
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| Co-Investigator(Kenkyū-buntansha) |
横井 匡 国立研究開発法人国立成育医療研究センター, 感覚器・形態外科部, 医師 (80514025)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 視神経 / 網膜神経節細胞 / iPS細胞 / ES細胞 / 再生医学 / 軸索 / 細胞移植 |
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| Outline of Final Research Achievements |
We generated self-induced retinal ganglion cells (RGCs) with functional axons from human and murine iPS and ES cells. Axons extended radially from the margin of the clump. Induced RGCs expressed specific markers by quantitative PCR and immunohistochemistry. The long, prominent axons contained neurofilaments and tau, and manifested anterograde axonal transport and sodium-dependent action potentials. The ability to generate RGCs with functional axons uniformly and at a high rate may contribute to treatment of various optic nerve diseases that threaten vision. We transplanted the RGCs into the murine retina after damaging the optic nerve. The RGCs were successfully engrafted and radiated their axons into the damaged optic nerve. Pathfinding of axons were in vitro evaluated by slow release of Sem3A and Slit1 from beads inserted in the colony of RGCs. Axons were bended in accordance with guidance of chemical agents.
These finding suggests a possibility of transplantation of RGCs.
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Report
(3 results)
Research Products
(52 results)
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[Journal Article] Clinical features of a toddler with bilateral bullous retinoschisis with a novel RS1 mutation.2017
Author(s)
Katagiri S, Tanaka S, Yokoi T, Hayashi T, Matsuzaka E, Ueda K, Yoshida-Uemura T, Arakawa A, Nishina S, Kadonosono K, Azuma N.
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Journal Title
American Journal of Ophthalmology Case Reports
Volume: 5
Pages: 76-80
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The mevalonate pathway regulates primitive streak formation via protein farnesylation2016
Author(s)
Okamoto-Uchida Y, Yu R, Miyamura N, Arima N, Ishigami-Yuasa M, Kagechika H, Yoshida S, Hosoya T, Nawa M, Kasama T, Asaoka Y, Alois RW, Elling U, Penninger JM, Nishina S, Azuma N, Nishina H
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Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 37697-37697
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] iPS細胞による視神経の研究2015
Author(s)
東 範行
Organizer
第36回西中国眼疾患フォーラム.
Place of Presentation
ANAクラウンプラザホテル宇部（ 山口・宇部市)
Year and Date
2015-11-26
Related Report
Invited
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[Presentation] 眼の発生と遺伝.2015
Author(s)
東 範行
Organizer
平成27年夏 合同症例検討会
Place of Presentation
順天堂大学（東京・文京区）
Year and Date
2015-08-01
Related Report
Invited
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