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Functional analysis of novel histone H3 variant H3mm7 in skeletal muscle differentiation

Research Project

Project/Area Number 15K18457
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Genome biology
Research InstitutionKyushu University

Principal Investigator

HARADA AKIHITO  九州大学, 生体防御医学研究所, 助教 (60596823)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsヒストンバリアント / 骨格筋分化 / エピジェネティクス
Outline of Final Research Achievements

The histone H3 variant H3.3 marks skeletal muscle specific genes in the undifferentiated state to provide selective gene expression during differentiation. Although some of the newly discovered histone variants have been shown to be highly expressed in skeletal muscle, their functions are still unknown. In this study, we performed functional analysis on one of these variants, H3mm7, in skeletal muscle. Using cultured cells, it was revealed that a rate change in gene expression occurs when H3mm7 is incorporated into the gene. In addition, the structure of the nucleosome containing H3mm7 was found to be less stable than the H3.3 nucleosome. These results together suggest that H3mm7 facilitates the expression of skeletal muscle genes by destabilizing nucleosomes at these genes to promote skeletal muscle differentiation.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (20 results)

All 2017 2016 2015 Other

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 9 results,  Acknowledgement Compliant: 5 results) Presentation (8 results) (of which Invited: 4 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Periostin Promotes Scar Formation through the Interaction between Pericytes and Infiltrating Monocytes/Macrophages after Spinal Cord Injury.2017

    • Author(s)
      Yokota K, Kobayakawa K, Saito T, Hara M, Kijima K, Ohkawa Y, Harada A, Okazaki K, Ishihara K, Yoshida S, Kudo A, Iwamoto Y, Okada S.
    • Journal Title

      Am J Pathol.

      Volume: 187 Issue: 3 Pages: 639-653

    • DOI

      10.1016/j.ajpath.2016.11.010

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis2017

    • Author(s)
      Ueda J., Harada A., Urahama T., Machida S., Maehara K., Hada M., Makino Y., Nogami J., Horikoshi N., Osakabe A., Taguchi H., Tanaka H., Tachiwana H., Yao T., Yamada M., Iwamoto T., Isotani A., Ikawa M., Tachibana T., Okada Y., Kimura H., Ohkawa Y., Kurumizaka H., Yamagata K.
    • Journal Title

      Cell Reports

      Volume: 18(3) Issue: 3 Pages: 593-600

    • DOI

      10.1016/j.celrep.2016.12.065

    • NAID

      120006957416

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of Immunoglobulin Gene Sequences from a Small Read Number of mRNA-Seq Using Hybridomas.2016

    • Author(s)
      Kuniyoshi Y, Maehara K, Iwasaki T, Hayashi M, Semba Y, Fujita M, Sato Y, Kimura H, Harada A, Ohkawa Y.
    • Journal Title

      PLoS One

      Volume: 11 Issue: 10 Pages: e0165473-e0165473

    • DOI

      10.1371/journal.pone.0165473

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Histone H4 lysine 20 acetylation is associated with gene repression in human cells.2016

    • Author(s)
      Kaimori, J. Y., Maehara, K., Hayashi-Takanaka, Y., Harada, A., Fukuda, M., Yamamoto, S., Ichimaru, N., Umehara, T., Yokoyama, S., Matsuda, R., Ikura, T., Nagao, K., Obuse, C., Nozaki, N., Takahara, S., Takao, T., Ohkawa, Y., Kimura, H., and Isaka, Y.
    • Journal Title

      Sci Rep.

      Volume: 6 Issue: 1 Pages: 24318-24318

    • DOI

      10.1038/srep24318

    • NAID

      120005770760

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Histone H3.5 forms an unstable nucleosome and accumulates around transcription start sites in human testis.2016

    • Author(s)
      Urahama T, Harada A, Maehara K, Horikoshi N, Sato K, Sato Y, Shiraishi K, Sugino N, Osakabe A, Tachiwana H, Kagawa W, Kimura H, Ohkawa Y, Kurumizaka H.
    • Journal Title

      Epigenetics Chromatin

      Volume: 9 Issue: 1 Pages: 1-16

    • DOI

      10.1186/s13072-016-0051-y

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chd5 Regulates MuERV-L/MERVL Expression in Mouse Embryonic Stem Cells Via H3K27me3 Modification and Histone H3.1/H3.2.2015

    • Author(s)
      Hayashi M, Maehara K, Harada A, Semba Y, Kudo K, Takahashi H, Oki S, Meno C, Ichiyanagi K, Akashi K, Ohkawa Y.
    • Journal Title

      J Cell Biochem

      Volume: 117 Issue: 3 Pages: 780-792

    • DOI

      10.1002/jcb.25368

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Distribution of histone H4 modifications as revealed by a panel of specific monoclonal antibodies.2015

    • Author(s)
      Hayashi-Takanaka Y., Maehara K., Harada A., Umehara T., Yokoyama S., Obuse C., Ohkawa Y., Nozaki N., Kimura H.
    • Journal Title

      Chromosome Res.

      Volume: 23 Issue: 4 Pages: 753-766

    • DOI

      10.1007/s10577-015-9486-4

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Relationship between the risk for a shrimp allergy and freshness or cooking2015

    • Author(s)
      Masakatsu USUI, Akihito HARADA, Shinya YASUMOTO, Yoshimasa SUGIURA, Anri NISHIDAI, Maria IKARASHI, Honami TAKABA, Taiko MIYASAKI, Hiroyuki AZAKAMI, and Masakazu KONDO
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 未定 Issue: 10 Pages: 1698-1701

    • DOI

      10.1080/09168451.2015.1045830

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Tissue-specific expression of histone H3 variants diversified after species separation.2015

    • Author(s)
      Maehara K, Harada A, Sato Y, Matsumoto M, Nakayama KI, Kimura H, Ohkawa Y.
    • Journal Title

      Epigenetics Chromatin

      Volume: 8 Issue: 1 Pages: 1-17

    • DOI

      10.1186/s13072-015-0027-3

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 急性期の筋萎縮過程における遺伝子発現変動解析2017

    • Author(s)
      原田 哲仁,岩崎 健,横田 和也,岡田 誠司,大川 恭行
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大(京都府)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] Functional switching of histone H3 variants in myogenesis2017

    • Author(s)
      原田哲仁
    • Organizer
      第4回ヒストンバリアント研究会
    • Place of Presentation
      東北大学青葉山新キャンパス 青葉山コモンズ
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] エピジェネティック破綻により引き起こされた筋肉腫の解析2017

    • Author(s)
      原田哲仁
    • Organizer
      公益財団法人福岡県すこやか健康事業団平成28年度がん研究助成金優秀賞受賞講演
    • Place of Presentation
      西鉄グランドホテル(福岡県福岡市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ヒストンH3バリアントの発現変動は筋萎縮過程の初期イベントである2016

    • Author(s)
      原田 哲仁, 岩崎 健, 横田 和也, 岡田 誠司, 大川 恭行
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 骨格筋分化におけるクロマチン構造解析-トランスクリプトミクスで理解する骨格筋形成2016

    • Author(s)
      原田哲仁
    • Organizer
      日本農芸化学会中四国支部 支部創立15周年記念 第24回若手シンポジウム
    • Place of Presentation
      水産大学校 (山口県下関市)
    • Year and Date
      2016-07-28
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 新規ヒストンH3バリアントH3mm7は骨格筋分化能を制御する2016

    • Author(s)
      原田 哲仁
    • Organizer
      日本農芸化学会 2016 大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-29
    • Related Report
      2015 Research-status Report
  • [Presentation] 新規ヒストンH3バリアントH3mm7は骨格筋分化能を制御する2015

    • Author(s)
      原田 哲仁
    • Organizer
      第38回日本分子生物学年会
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] ヒストンバリアントが制御する骨格筋分化能2015

    • Author(s)
      原田 哲仁、前原 一満、大川 恭行
    • Organizer
      NGS現場の会第四回研究会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2015-07-01
    • Related Report
      2015 Research-status Report
    • Invited
  • [Book] ヒストンバリアントによるエピゲノム制御2015

    • Author(s)
      原田 哲仁
    • Total Pages
      4
    • Publisher
      ㈱メディカルレビュー社
    • Related Report
      2015 Research-status Report
  • [Remarks] トランスクリプトミクス分野 / 大川研究室

    • URL

      http://tx.bioreg.kyushu-u.ac.jp/

    • Related Report
      2015 Research-status Report
  • [Patent(Industrial Property Rights)] DNA結合タンパク質の結合領域の近傍に所望のDNA断片を挿入する方法2016

    • Inventor(s)
      大川恭行,原田哲仁,胡桃坂仁志,木村宏,半田哲也,他2名
    • Industrial Property Rights Holder
      国立大学法人九州大学、国立大学法人東京工業大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-167967
    • Filing Date
      2016-08-30
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2018-03-22  

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