Revealing unknown basal fungal groups abundantly detected in deep-sea environments

• Project/Area Number: 15K18601
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biodiversity/Systematics
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: NAGANO Yuriko 国立研究開発法人海洋研究開発機構, 地球環境部門(海洋生物環境影響研究センター), 研究員 (30512917)
• Project Period (FY): 2015-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 真菌類 / 深海 / 化学合成生態系 / 原始的菌類 / 鯨骨 / 菌類 / Cryptomycota / 真菌 / 深海熱水域 / アスファルト湧出域 / 微生物 / 海洋生態 / 深海環境

Outline of Final Research Achievements

Fungal diversity, including unknown basal fungal groups in deep-sea chemosynthetic communities was investigated using next-generation sequencing. Through this study, the fungal diversity in deep-sea environments and its novelty, the domination of unknown basal fungal groups, not only in Sagami Bay methane cold-seep but also in the whale-fall chemosynthetic ecosystems were revealed. Furthermore, through collaborative research, novel compounds with useful physiological functions, such as anti-oomycete, anti-tumor, and antioxidant, was discovered from fungi isolated from the deep-sea environments.

Academic Significance and Societal Importance of the Research Achievements

本研究を通して深海環境、特に深海化学合成生態系から多量検出される原始的真菌群の多様性や存在傾向などが明らかとなった。原始的真菌群は菌類の初期進化を理解する上で非常に重要なグループであり、また深海環境中の真菌を対象とした研究は国内外を通して非常にユニークな研究であると言える。
さらに、真菌類は古くから抗生物質を始めとした人類に有用な生理活性物質を産生する事で知られており、深海環境中に生息する真菌類は生物資源としても非常に貴重なリソースである。本研究では数多くの深海由来分離株を取得できたほか、有用機能を持った新規化合物の発見にも繋がった。

Research Products

• [Int'l Joint Research] University of Sao Paulo/Federal University of Sao Paulo/University of Vale do Itajai(Brazil)
• [Journal Article] Bacterial diversity in deep-sea sediments under influence of asphalt seep at the Sao Paulo Plateau (2020)
  • Author(s): Queiroz Luciano Lopes、Bendia Amanda Goncalves、Duarte Rubens Tadeu Delgado、das Gracas Diego Assis、da Costa da Silva Artur Luiz、Nakayama Cristina Rossi、Sumida Paulo Yukio、Lima Andre O. S.、Nagano Yuriko、Fujikura Katsunori、Kitazato Hiroshi、Pellizari Vivian Helena
  • Journal Title: Antonie van Leeuwenhoek Volume: 113 Issue: 5 Pages: 707-717
  • DOI: 10.1007/s10482-020-01384-8
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Deep-sea endemic fungi? The discovery of Alisea longicolla from artificially immersed wood in deep sea off the Nansei Islands, Japan (2019)
  • Author(s): Nagano Yuriko、Fujiwara Yoshihiro、Nishimoto Atsushi、Haga Takuma、Fujikura Katsunori
  • Journal Title: Mycoscience Volume: 60 Issue: 4 Pages: 228-231
  • DOI: 10.1016/j.myc.2019.03.004
  • NAID: 40021955246
  • Peer Reviewed
• [Journal Article] Cipralphelin, a new anti-oxidative N-cinnamoyl tripeptide produced by the deep sea-derived fungal strain Penicillium brevicompactum FKJ-0123 (2019)
  • Author(s): Matsuo Hirotaka、Mokudai Takayuki、Higo Mayuka、Nonaka Kenichi、Nagano Yuriko、Nagahama Takahiko、Niwano Yoshimi、Takahashi Yoko、Omura Satoshi、Nakashima Takuji
  • Journal Title: The Journal of Antibiotics Volume: 72 Issue: 10 Pages: 775-778
  • DOI: 10.1038/s41429-019-0208-6
  • Peer Reviewed
• [Journal Article] New metabolites, sarcopodinols A and B, isolated from deep-sea derived fungal strain Sarcopodium sp. FKJ-0025 (2018)
  • Author(s): Matsuo Hirotaka、Nonaka Kenichi、Nagano Yuriko、Yabuki Akinori、Fujikura Katsunori、Takahashi Yoko、Omura Satoshi、Nakashima Takuji
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 82 Issue: 8 Pages: 1323-1326
  • DOI: 10.1080/09168451.2018.1467264
  • Peer Reviewed
• [Journal Article] Quellenin, a new anti-Saprolegnia compound isolated from the deep-sea fungus, Aspergillus sp. YK-76 (2018)
  • Author(s): Takahashi K, Sakai K, Fukasawa W, Nagano Y, Sakaguchi SO, Lima AO, Pellizari VH, Iwatsuki M, Takishita K, Yoshida T, Nonaka K, Fujikura K, Omura S
  • Journal Title: Journal of Antibiotics Volume: 71 Issue: 8 Pages: 741-744
  • DOI: 10.1038/s41429-018-0053-z
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Fungal diversity in deep-sea sediments associated with asphalt seeps at the Sao Paulo Plateau (2017)
  • Author(s): Nagano Yuriko、Miura Toshiko、Nishi Shinro、Lima Andre O.、Nakayama Cristina、Pellizari Vivian H.、Fujikura Katsunori
  • Journal Title: Deep Sea Research Part II: Topical Studies in Oceanography Volume: 146 Pages: 59-67
  • DOI: 10.1016/j.dsr2.2017.05.012
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cladomarine, a new anti-saprolegniasis compound isolated from the deep-sea fungus, Penicillium coralligerum YK-247 (2017)
  • Author(s): Takahashi Konami、Sakai Kazunari、Nagano Yuriko、Orui Sakaguchi Sakiko、Lima Andre O、Pellizari Vivian H、Iwatsuki Masato、Takishita Kiyotaka、Nonaka Kenichi、Fujikura Katsunori、?mura Satoshi
  • Journal Title: The Journal of Antibiotics Volume: 70 Issue: 8 Pages: 911-914
  • DOI: 10.1038/ja.2017.58
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Retrieval of deeply buried culturable fungi in marine subsurface sediments, Suruga-Bay, Japan (2016)
  • Author(s): Yuriko Nagano,Masaaki Konishi, Takahiko Nagahama, Takaaki Kubota, Fumiyoshi Abe, Yuji Hatada
  • Journal Title: Fungal Ecology Volume: 20 Pages: 256-259
  • DOI: 10.1016/j.funeco.2015.12.012
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 深海に設置した木材より採集された深海生菌類Alisea longicolla (2019)
  • Author(s): 長野由梨子・藤原義弘・西本篤史・芳賀拓真・藤倉克則
  • Organizer: 日本菌学会第63回大会
• [Presentation] Fungal diversity in whale fall chemosynthetic ecosystems (2019)
  • Author(s): Yuriko Nagano, Toshiko Miura, Andre O Lima, Taishi Tsubouchi, Masaru Kawato, Yoshihiro Fujiwara and Katsunori Fujikura
  • Organizer: 30th Fungal Genetics Conference
  • Int'l Joint Research
• [Presentation] Fungal diversity in deep-sea environments (2018)
  • Author(s): Yuriko Nagano
  • Organizer: The 15th International Marine & Freshwater Mycology Symposium
  • Int'l Joint Research / Invited
• [Presentation] 深海由来糸状菌培養物からの抗ミズカビ化合物の探索 (2017)
  • Author(s): 高橋こなみ、酒井一成、長野由梨子、大類穂子、Andre O Lima、Vivian H. Pellizari、岩月正人、瀧下清貴、野中健一、藤倉克則、大村 智
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都女子大学、京都府京都市
  • Year and Date: 2017-03-19
• [Presentation] 深海由来糸状菌培養物からの抗ミズカビ化合物の探索 (2017)
  • Author(s): 高橋こなみ、酒井一成、長野由梨子、大類穂子、Andre O Lima、Vivian H. Pellizari、岩月正人、瀧下清貴、野中健一、藤倉克則、大村 智
  • Organizer: ブルーアース2017
  • Place of Presentation: 日本大学理工学部、東京都千代田区
  • Year and Date: 2017-03-03
• [Presentation] 深海由来真菌の分子同定および抗生物質の探索 (2016)
  • Author(s): 高橋こなみ、長野由梨子、瀧下清貴、藤倉克則
  • Organizer: 日本菌学会60周年記念大会
  • Place of Presentation: 京都大学農学部、京都府京都市
  • Year and Date: 2016-09-18
• [Presentation] 深海由来真菌の分子同定および抗生物質の探索 (2016)
  • Author(s): 高橋こなみ,長野由梨子, 瀧下清貴, 藤倉克則, 横田賢史
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 札幌コンベンションセンター、北海道札幌市
  • Year and Date: 2016-03-29
• [Presentation] Unprecedented fungal diversity revealed in whale fall chemosynthetic ecosystems (2015)
  • Author(s): Yuriko Nagano, Toshiko Miura, Andre O Lima, Taishi Tsubouchi, Masaru Kawato, Yoshihiro Fujiwara and Katsunori Fujikura
  • Organizer: Asian mycology Congress 2015
  • Place of Presentation: Goa University, Goa, India
  • Year and Date: 2015-10-09
  • Int'l Joint Research
• [Presentation] Fungal diversity in asphalt seep at the Sao Paulo Plateau (2015)
  • Author(s): Nagano Yuriko, Miura Toshiko, Nishi Shinro, Fujikura Katsunori
  • Organizer: Annual meeting of Mycological society of America in Botany 2015
  • Place of Presentation: Shaw Conference Centre, Edmonton, Canada
  • Year and Date: 2015-07-27
  • Int'l Joint Research