| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Nucleoid-associated proteins (NAPs), which fold bacterial DNA and influence gene transcription, are global transcriptional regulators of genes on both plasmids and the host chromosome. The catabolic plasmid pCAR1 carries three genes encoding NAPs: MvaT homologue (H-NS family protein) Pmr, NdpA homologue Pnd, and HU homologue Phu. In this study, we used Pseudomonas putida KT2440 as a host of pCAR1 and clarified the functions of HU and NdpA homologues. KT2440(pCAR1) forms flat and filamentous biofilms, while enhanced filamentous morphology was observed when pmr and pnd, or pmr and phu, were simultaneously disrupted. Three genes on the chromosome (PP_0308, PP_0309, and PP_2193) were suggested to be related with the biofilm morphology. Furthermore, we found that car gene cluster, which is responsible for degradation of carbazole, were always lost in the double mutant of pnd and PP_0973 (encoding an NdpA homologue), suggesting that NdpA homologues affect fitness of the host.
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