Application of RNA interference in Screlactinian corals.
Project/Area Number |
15K18744
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Aquatic life science
|
Research Institution | University of Tsukuba (2016-2017) National Institute of Genetics (2015) |
Principal Investigator |
YUYAMA Ikuko 筑波大学, 生命環境系, 特任助教 (80565995)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 造礁性サンゴ / 遺伝子ノックダウン / 細胞内共生 / RNAi / サンゴ / 骨格形成 / 褐虫藻 / GFP / Thioredoxin |
Outline of Final Research Achievements |
To date, only few studies have used RNA interference technology to Scleractinian corals. The aimed of this research is optimization and establishment of RNAi interference mediated gene knockdown in Scleractinian corals. The results demonstrated that RNAi causes down-regulation of expression of target genes and indicated that RNAi is an effective method for screening for gene function involved in coral-algal endosymbiosis and coral stress response.
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Report
(4 results)
Research Products
(21 results)