Analysis of TCR signaling dependent mDia activation molecular mechanism
Project/Area Number |
15K18986
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
General pharmacology
|
Research Institution | Kyoto University |
Principal Investigator |
Thumkeo Dean 京都大学, 医学研究科, 特定准教授 (40372594)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | T細胞 / mDia / TCRシグナル / アクチン / 免疫抑制剤 / 低分子量G蛋白質 |
Outline of Final Research Achievements |
T cell is essential for adaptive immunity. T cells is activated by TCR signaling that is indispensable for T cell development and function. I have previously reported that mDia facilitates TCR signaling through the regulation of actin polymerization by utilizing mDia 1/3 double deficient mice. In this study, I further unraveled the molecular mechanism of mDia activation in TCR signaling. We found that mDia is activated by the small G protein RhoA upon TCR stimulation. In addition, using T cells expressing EGFP-mDia3, I found that mDia is localized at the cell periphery in T cells upon TCR stimulation. Therefore, mDia is activated upon TCR stimulation through RhoA at the cell periphery and facilitate TCR signaling through its actin polymerization activity.
|
Report
(3 results)
Research Products
(10 results)
-
[Journal Article] Constitutive activation of DIA1 (DIAPH1) via C-terminal truncation causes human sensorineural hearing loss2016
Author(s)
Ueyama T, Ninoyu Y, Nishio S, Miyoshi T, Torii H, Nishimura K, Sugahara K, Sakata H, Thumkeo D, Sakaguchi H, Watanabe N, Usami S, Saito N, Kitajiri S
-
Journal Title
EMBO Molecular Medicine
Volume: 8
Pages: 1310-1324
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
[Presentation] Role of mDia in spermatogenesis2015
Author(s)
Satoko Sakamoto, Dean Thumkeo, Masahito Ikawa, Yoshitaka Fujiwara, Hiroshi Ohta, Sadanori Watanabe, Shuh Narumiya
Organizer
第67回日本細胞生物学会
Place of Presentation
東京
Year and Date
2015-07-02
Related Report