| Project/Area Number |
15K19075
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Experimental pathology
|
|---|
| Research Institution | Tohoku University (2016)
Kyoto University (2015) |
|---|
Principal Investigator |
Ito Koyu 東北大学, 加齢医学研究所, 助教 (90609497)
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | オステオポンチン / 腸内細菌叢 / 腸管上皮内リンパ球 |
|---|
| Outline of Final Research Achievements |
In this study, using Opn-EGFP knock-in (KI) mice we found that CD8α+ T cells in the intestinal tissues, including Peyer’s patch, lamina propria and epithelium, express Opn under steady state conditions. Among these cell, intraepithelial lymphocyte (IEL) CD8 T cells shows highest expression of Opn, and majority of these cells are TCRγδ cells. Opn knockout (KO) mice had altered fecal microflora concordant with a reduction of TCRγδ+ IELs. Consistent with this result, both treatment with anti-Opn blocking antibody and deficiency of Opn resulted in decreased survival of TCRγδ+ and TCRαβ+ IELs. This data suggests that a possibility that Opn may function as a survival factor for IELs in the intestinal tissue.
|
