| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
|---|
| Outline of Final Research Achievements |
A decellularization protocol for the trachea of the mouse was prepared according to the past papers and we made decellularized trachea. Although there was no cell remnant observed, the strength of the trachea was not preserved. That is why we revised the protocol and rebuilt decellularized trachea. It was succeeded in creating a decellularized trachea with strength maintained almost without observing the residual of the cell. We could create a minimum acceptable protocol and it can be a foothold for regeneration trachea creation.
Mesenchymal stem cells were set as a cell source for regenerating trachea, and a difference in cartilage differentiation ability was observed between donors. As a result of examination focusing on Epigenetics, we found a difference in chromatin structure, suggesting the possibility of being the cause of the difference in cartilage differentiation potential.
|
