| Project/Area Number |
15K19687
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Osaka Ohtani University |
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Principal Investigator |
Ikebuchi Ryoyo 大阪大谷大学, その他部局等, 特別研究員PD (00747529)
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| Research Collaborator |
TOMURA Michio 大阪大谷大学, 薬学部, 教授 (30314321)
KUSUMOTO Yutaka 大阪大谷大学, 薬学部, 准教授 (40252689)
MORIYA Taiki 大阪大谷大学, 薬学部, 助教 (30759759)
TERAGUCHI Shunsuke 東北大学, 東北メディカルメガバンク機構, 助教 (00467276)
Alexis Vandenbon 大阪大学, 免疫学フロンティア研究センター, 助教 (60570140)
NAKAYAMA Takashi 近畿大学, 薬学部, 教授 (60319663)
MATSUO Kazuhiko 近畿大学, 薬学部, 講師 (70615921)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 制御性T細胞 / 炎症性皮膚疾患 / 免疫抑制 / シングルセル解析 / 細胞遊走 / KikGRマウス / 接触性皮膚炎 / ケモカイン |
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| Outline of Final Research Achievements |
We identified several regulatory T cell (Treg) subsets in inflamed skin tissue of mouse line expressing photoconvertible protein KikGR, by which we can distinguish skin-coming and -remaining lymphocytes, by multi-parameter single-cell gene and protein expression analysis. Each subset expressed each set of functional and migration-related molecules and showed different capabilities to remain in inflamed skin. We distinguished skin-coming and -remaining Tregs without KikGR expression data by multi-parameter single-cell expression analysis of functional and migration-related molecules. We also tried to identify master molecules of Treg subset differentiation and establish method to collect the Treg subsets. This research suggests that Tregs expressing different functional molecules showed different migration status in inflamed tissues.
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