| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Paracetamol (PARA) is an antipyretic and analgesic medicine widely used in many countries. However, the precise molecular targets of PARA and the mechanisms of antipyretic and analgesic effects by PARA have not been fully clarified yet.
The PARA-compound and Hela cell lysate were bound and reacted. A band of the proteins bound to PARA-immobilized beads was GSK-3β , which is involved in body temperature homeostasis. The protein was stained with the antibody of GSK-3β by Western Blotting. Luminescent signals, which is correlated with the amount of GSK-3β activity, significantly decreased with increasing dose of PARA. These results indicate that PARA binds GSK-3β and inhibits its activity at clinically relevant concentrations.
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