Analysis of interaction between spermatogonial stem cell and Sertoli cell against male infertility

• Project/Area Number: 15K20103
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Nagoya City University
• Principal Investigator: Hideyuki Kamisawa 名古屋市立大学, 大学院医学研究科, 研究員 (00551277)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 精子幹細胞 / 男性不妊症 / 停留精巣 / セルトリ細胞 / UTF1 / アポトーシス

Outline of Final Research Achievements

Male infertility is one of the most serious problem in our contry. We previously reported that spermatogonial stem cell (SSC) activity indicated future male fertility. However testicular histopathology and molecular mechanism against poor spermatogenesis were not elucidated. In this study, we performed human testicular biopsy and examined SSC funtion and Sertoli cells against risk disease of congenital male infertility. Our study showed that SSC's activity in cryptorchid testes which induced male infertility was already harmed in infant term. Moreover, in adult testes with past history of cryptorchidism might harm testicular function by SSCs activity loss, activation of apoptosis and disturbance of maturation of Sertoli cells.

Research Products

• [Journal Article] 停留精巣固定術の術前・術後管理 (2017)
  • Author(s): 林 祐太郎、岩月 正一郎、神沢 英幸
  • Journal Title: 泌尿器Care&Cure Uro-Lo Volume: 22 Pages: 103-107
  • Peer Reviewed
• [Journal Article] A rare case of epididymal abscess in an infant treated successufully with needle aspiraton. (2017)
  • Author(s): Nishio Hidenori, Mizuno Kentaro, Kato Taiki, Moritoki Yoshinobu, Kamisawa Hideyuki, Kurokawa Satoshi, Nakane Akihiro, Okada Atsushi, Yasui Takahiro, Hayashi Yutaro
  • Journal Title: Urology Case Reports Volume: 13 Pages: 26-27
  • DOI: 10.1016/j.eucr.2017.03.012
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 膀胱尿管逆流術後の尿中N-アセチル-β-D-グルコサミニダーゼ高値持続と分腎機能低下 (2017)
  • Author(s): 丸山 哲史、西尾 英紀、守時 良演、加藤 大貴、神沢 英幸、黒川 覚史、中根 明宏、水野 健太郎、林 祐太郎、安井 孝周
  • Journal Title: 日本小児泌尿器科学会誌 Volume: 26 Pages: 47-54
  • Peer Reviewed
• [Journal Article] Involvement of the bone morphogenic protein/SMAD signaling pathway in the etiology of congenital anomalies of the kidney and urinary tract accompanied by cryptorchidism (2017)
  • Author(s): Mizuno Kentaro、Nakane Akihiro、Nishio Hidenori、Moritoki Yoshinobu、Kamisawa Hideyuki、Kurokawa Satoshi、Kato Taiki、Ando Ryosuke、Maruyama Tetsuji、Yasui Takahiro、Hayashi Yutaro
  • Journal Title: BMC Urology Volume: 17 Issue: 1 Pages: 1-6
  • DOI: 10.1186/s12894-017-0300-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 停留精巣・遊走精巣の診断および治療戦略 (2017)
  • Author(s): 神沢 英幸、林 祐太郎
  • Journal Title: 小児外科 Volume: 49 Pages: 1040-1044
  • Peer Reviewed
• [Journal Article] Genome-wide gene expression profiling of Randall's plaques in calcium oxalate stone formers (2017)
  • Author(s): Taguchi K, Hamamoto S, Okada A, Unno R, Kamisawa H, Naiki T, Ando R, Mizuno K, Kawai N, Tozawa K, Kohri K, Yasui T
  • Journal Title: J Am Soc Nephrol Volume: 28 Issue: 1 Pages: 333-347
  • DOI: 10.1681/asn.2015111271
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 腎・泌尿器科疾患、生殖器疾患 停留精巣 (2017)
  • Author(s): 林 祐太郎、水野 健太郎、守時 良演、西尾 英紀、加藤 大貴、神沢 英幸
  • Journal Title: 小児科診療 Volume: 80 Pages: 355-357
  • Peer Reviewed
• [Journal Article] Robot assisted laparoscopic pyeloplasty for ureteropelvic junction obstruction: Comparison between pediatric and adult patient - Japanese series. (2016)
  • Author(s): Mizuno K, Kojima Y, Kurokawa S, Kamisawa H, Nishio H, Moritoki Y, Nakane A, Maruyama T, Okada A, Kawai N, Tozawa K, Kohri K, Yasui T, Hayashi Y.
  • Journal Title: Journal of Robotic Surgery Volume: 印刷中 Issue: 2 Pages: 151-157
  • DOI: 10.1007/s11701-016-0633-5
  • Peer Reviewed
• [Journal Article] Adrenal neuroblastoma in an adult: effect of radiotherapy on local progression after surgical removal. (2016)
  • Author(s): Kurokawa Satoshi, Mizuno Kentaro, Nakane Akihiro, Moritoki Yoshinobu, Nishio Hidenori, Kamisawa Hideyuki, Kubota Yasue, Okada Atsushi, Kawai Noriyasu, Hayashi Yutaro, Yasui Takahiro
  • Journal Title: Case Reports in Urology Volume: - Pages: 1-4
  • DOI: 10.1155/2016/2657632
  • Peer Reviewed / Open Access
• [Journal Article] 停留精巣の諸問題―早期手術の有用性と問題点― (2016)
  • Author(s): 水野 健太郎、西尾 英紀、守時 良演、加藤 大貴、神沢 英幸、林 祐太郎
  • Journal Title: 泌尿器外科 Volume: 29 Pages: 1107-1114
  • Peer Reviewed
• [Journal Article] ラットおよびヒトの停留精巣におけるヒストン脱メチル化酵素Kdm5aと造精機能障害 (2016)
  • Author(s): 西尾 英紀、最上 徹、水野 健太郎、守時 良演、神沢 英幸、中根 明宏、黒川 覚史、丸山 哲史、佐々木 昌一、安井 孝周、林 祐太郎
  • Journal Title: 日本小児泌尿器科学会雑誌 Volume: 25 Pages: 4-9
  • Peer Reviewed
• [Journal Article] Hemiscrotumの1例；発症機序の解明と手術方法の検討 (2016)
  • Author(s): 西尾英紀、水野健太郎、守時良演、神沢英幸、内木拓、黒川覚史、中根明宏、岡田淳志、安井孝周、林祐太郎
  • Journal Title: 日本小児泌尿器科学会誌 Volume: 25 Pages: 18-18
  • Peer Reviewed
• [Journal Article] Hemiscrotal agenesis: Pathogenesis and management strategies. (2015)
  • Author(s): Nishio Hidenori, Mizuno Kentaro, Moritoki Yoshinobu, Kamisawa Hideyuki, Naiki Taku, Kurokawa Satoshi, Nakane Akihiro, Okada Atsushi, Yasui Takahiro, Hayashi Yutaro
  • Journal Title: International Journal of Urology Volume: 69(10) Issue: 6 Pages: 824-831
  • DOI: 10.1111/iju.13079
  • Peer Reviewed
• [Journal Article] 先天性および小児泌尿器疾患 停留精巣 (2015)
  • Author(s): 神沢 英幸、藤田 圭治
  • Journal Title: 臨床泌尿器科 Volume: 69 Pages: 345-348
  • Peer Reviewed
• [Journal Article] 先天性および小児泌尿器疾患 非触知精巣 (2015)
  • Author(s): 神沢 英幸、藤田 圭治
  • Journal Title: 臨床泌尿器科 Volume: 69 Pages: 349-352
  • Peer Reviewed
• [Journal Article] 特集 停留精巣：最近の知見 Fowler-Stephens法による精巣固定術と成績 (2015)
  • Author(s): 黒川 覚史、神沢 英幸、中根 明宏、林 祐太郎
  • Journal Title: 小児外科 Volume: 47 Pages: 848-852
  • Peer Reviewed
• [Journal Article] Clear cell sarcoma of the kidney distinguished from synovial sarcoma of the kidney by genetic analysis (2015)
  • Author(s): Hirose M, Mizuno K, Kamisawa H, Nishio H, Moritoki Y, Kohri K, Hayashi Y.
  • Journal Title: BMC Research Notes Volume: 8 Issue: 1 Pages: 129-133
  • DOI: 10.1186/s13104-015-1100-5
  • Peer Reviewed / Open Access
• [Presentation] Fowler-Stephens法による精巣固定術が行われた腹腔内停留精巣の長期予後 (2017)
  • Author(s): 神沢 英幸、西尾 英紀、水野 健太郎、丸山 哲史、安井 孝周、 林 祐太郎
  • Organizer: 第105回日本泌尿器科学会総会
• [Presentation] Treatment indication of the infant phimosis judging from a penile foreskin natural history - the analysis of 1244 boys -. (2017)
  • Author(s): Kurokawa Satoshi, Mizuno Kentaro, Kamisawa Hideyuki, Moritoki Yoshinobu, Nishio Hidenori, Nakane Akihiro, Maruyama Tetsuji, Hayashi Yutaro, Yasui Takahiro
  • Organizer: 2017 (65th) Annual Meeting of The Society for Pediatric Urology
  • Int'l Joint Research
• [Presentation] Kdm5a inhibits gonocyte differentiation to spermatogonial stem cells by suppressing Ret gene function via epigenetic regulation. (2017)
  • Author(s): Nishio Hidenori, Kato Taiki, Mizuno Kentaro, Moritoki Yoshinobu, Kamisawa Hideyuki, Kurokawa Satoshi, Nakane Akihiro, Maruyama Tetsuji, Hayashi Yutaro, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2017
  • Int'l Joint Research
• [Presentation] Testicular histology in infants with prader-willi syndrome. (2017)
  • Author(s): Kato Taiki, Mizuno Kentaro, Nishio Hidenori, Moritoki Yoshinobu, Nakane Akihiro, Kamisawa Hideyuki, Kurokawa Satoshi, Maruyama Tetsuji, Yasui Takahiro, Hayashi Yutaro
  • Organizer: American Urological Association Annual Meeting 2017
  • Int'l Joint Research
• [Presentation] 遊走精巣の治療指針：遺伝子解析は手術適応決定の決め手になるか？ (2017)
  • Author(s): 神沢 英幸、林 祐太郎
  • Organizer: 第26回日本小児泌尿器科学会総会・学術集会
• [Presentation] 精巣固定術を適切な時期に行うためにできること～停留精巣患児523例の背景検討～ (2017)
  • Author(s): 神沢 英幸、丸山 哲史、安井 孝周、林 祐太郎
  • Organizer: 第26回日本小児泌尿器科学会総会・学術集会
• [Presentation] 海外招請の講師からみた日本小児泌尿器科学会の歴史 (2017)
  • Author(s): 神沢 英幸、林 祐太郎
  • Organizer: 第26回日本小児泌尿器科学会総会・学術集会
• [Presentation] ホルミウムレーザーを用いて尿管瘤切開術と瘤内結石破砕術を施行した1例 (2017)
  • Author(s): 神沢 英幸、太田 裕也、池上 要介、丸山 哲史、安井 孝周、林 祐太郎
  • Organizer: 第31回日本泌尿器内視鏡学会総会
• [Presentation] 早期に精巣固定術が行われなかった停留精巣症例の背景因子の解析 (2016)
  • Author(s): 神沢 英幸、田口 和己
  • Organizer: 第25回日本小児泌尿器科学会総会・学術集会
  • Place of Presentation: ホテルグランヴィア京都(京都府京都市)
  • Year and Date: 2016-06-28
• [Presentation] 男性不妊症のメカニズム 男性不妊症における精子幹細胞の性能異常と先天性要因の関与 (2016)
  • Author(s): 神沢 英幸、西尾 英紀、守時 良演、岩月 正一郎、水野 健太郎、梅本 幸裕、神谷 浩行、佐々木 昌一、林 祐太郎、安井 孝周
  • Organizer: 日本アンドロロジー学会第35回学術大会
  • Place of Presentation: 前橋テルサ(群馬県前橋市)
  • Year and Date: 2016-06-24
  • Invited
• [Presentation] Discovery of Randall’s plaque gene expression profiling in calcium oxalate stone formers by immunohistochemical genome-wide analysis. (2016)
  • Author(s): Taguchi Kazumi, Hamamoto Shuzo, Okada Atsushi, Unno Rei, Kamisawa Hideyuki, Naiki Taku, Ando Ryosuke, Tozawa Keiichi, Kohri Kenjiro, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2016
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-05-06
  • Int'l Joint Research
• [Presentation] Kdm5a, a histone H3K4 demethylase, regulates spermatogenesis via its action on germ cell. (2016)
  • Author(s): Nishio Hidenori, Mizuno Kentaro, Moritoki Yoshinobu, Kamisawa Hideyuki, Nakane Akihiro, Kurokawa Satoshi, Maruyama Tetsuji, Hayashi Yutaro, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2016
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-05-06
  • Int'l Joint Research
• [Presentation] Relationship between Sertoli cell function and differentiateon process of spermatogonial stem cells in the cryptorchid testes. (2016)
  • Author(s): Nagai Takashi, Mizuno Kentaro, Hayashi Yutaro, Kamisawa Hideyuki, Moritoki Yoshinobu, Nishio Hidenori, Kurokawa Satoshi, Nakane Akihiro, Maruyama Tetsuji, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2016
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-05-06
  • Int'l Joint Research
• [Presentation] Involvement of BMP-SMAD signaling pathway in the etiology of congenital anomalies of kidney and urinary tract accompanied by cryptorchidism using whole exsome sequencing. (2016)
  • Author(s): Mizuno Kentaro, Hayashi Yutaro, Kamisawa Hideyuki, Moritoki Yoshinobu, Nishio Hidenori, Kurokawa Satoshi, Nakane Akihiro, Maruyama Tetsuji, Yasui Takahiro
  • Organizer: Society for Pediatric Urology’s 64st Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-05-06
  • Int'l Joint Research
• [Presentation] Natural history and management of perinatally detected hydronephrosis, through long-term follow up. (2016)
  • Author(s): Akihiro Nakane, Hayashi Yutaro, Mizuno Kentaro, Nishio Hidenori, Moritoki Yoshinobu, Kamisawa Hideyuki, Kurokawa Satoshi, Maruyama Tetsuji, Yasui Takahiro
  • Organizer: Society for Pediatric Urology’s 64st Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-05-06
  • Int'l Joint Research
• [Presentation] Verification of the guideline for cryptorchism and perspectives for future therapies of testicular disease (2016)
  • Author(s): Kamisawa Hideyuki, Fujita Keiji, Hayashi Yutaro, Yasui Takahiro
  • Organizer: 第104回日本泌尿器科学会総会
  • Place of Presentation: 仙台国際センター他(宮城県仙台市)
  • Year and Date: 2016-04-23
• [Presentation] Discovery of a gene expression profile contributing to renal papillary calcification as Randall’s plaques by genome-wide analysis (2016)
  • Author(s): Taguchi Kazumi, Hamamoto Shuzo, Unno Rei, Kamisawa Hideyuki, Ando Ryosuke, Okada Atsushi, Umemoto Yukihiro, Kamiya Hiroyuki, Tozawa Keiichi, Kohri Kenjiro, Yasui Takahiro
  • Organizer: 第104回日本泌尿器科学会総会
  • Place of Presentation: 仙台国際センター他(宮城県仙台市)
  • Year and Date: 2016-04-23
• [Presentation] 泌尿器領域における小児・先天異常疾患の話題 (2016)
  • Author(s): 神沢 英幸
  • Organizer: 名古屋市南区医師会 南圭会 学術講演会
  • Place of Presentation: 南区休日急病診療所・南部平日夜間急病センター（愛知県名古屋市）
  • Year and Date: 2016-03-15
  • Invited
• [Presentation] 停留精巣が男性不妊症に至る機序の解明～閉塞性無精子症患者の精巣組織を用いた研究～ (2016)
  • Author(s): 神沢 英幸、林 祐太郎、岩月 正一郎、水野 健太郎、梅本 幸裕、佐々木 昌一、安井 孝周
  • Organizer: 第20回日本生殖内分泌学会学術集会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2016-01-09
• [Presentation] 外来で遭遇する小児泌尿器疾患-最低限これだけは知っておきたい最新情報- 停留精巣・遊走精巣 (2015)
  • Author(s): 神沢 英幸、藤田 圭治、水野 健太郎、林 祐太郎、安井 孝周
  • Organizer: 第65回日本泌尿器科学会中部総会
  • Place of Presentation: 長良川国際会議場、岐阜都ホテル（岐阜県岐阜市）
  • Year and Date: 2015-10-23
  • Invited
• [Presentation] 停留精巣と陰嚢水腫における手術時年齢と精巣発育との関連性 (2015)
  • Author(s): 神沢 英幸、藤田 圭治、林 祐太郎、水野 健太郎、西尾 英紀、守時 良演、安井 孝周
  • Organizer: 第65回日本泌尿器科学会中部総会
  • Place of Presentation: 長良川国際会議場、岐阜都ホテル（岐阜県岐阜市）
  • Year and Date: 2015-10-23
• [Presentation] 小児陰嚢内疾患 本邦「停留精巣診療ガイドライン(2005)」が小児治療に果たしてきた役割 (2015)
  • Author(s): 神沢 英幸
  • Organizer: 第24回日本小児泌尿器科学会総会・学術集会
  • Place of Presentation: 御茶ノ水ソラシティカンファレンスセンター（東京都千代田区）
  • Year and Date: 2015-07-01
  • Invited
• [Presentation] 停留精巣はなぜ不妊症の原因となるのか？～男性不妊症検体を用いた組織学的検討～ (2015)
  • Author(s): 神沢 英幸、水野 健太郎、岩月 正一郎、佐々木 昌一、林 祐太郎、安井 孝周
  • Organizer: 第24回日本小児泌尿器科学会総会・学術集会
  • Place of Presentation: 御茶ノ水ソラシティカンファレンスセンター（東京都千代田区）
  • Year and Date: 2015-07-01
• [Presentation] 停留精巣が男性不妊症に至る機序とは～閉塞性無精子症患者の精巣検体を用いた研究～ (2015)
  • Author(s): 神沢 英幸、水野 健太郎、西尾 英紀、守時 良演、岩月 正一郎、黒川 覚史、梅本 幸裕、佐々木 昌一、林 祐太郎、安井 孝周
  • Organizer: 第34回日本アンドロロジー学会学術大会
  • Place of Presentation: 福岡大学病院メディカルホール（福岡県福岡市）
  • Year and Date: 2015-06-26
• [Presentation] The space environment induces the enhancement of caveolin expression and leads to germ cell apoptosis. (2015)
  • Author(s): Kamisawa Hideyuki, Mizuno Kentaro, Kurokawa Satoshi, Moritoki Yoshinobu, Noshio Hidenori, Nakane Akihiro, Maruyama Tetsuji, Fujita Keiji, Sasaki Shoichi, Hayashi Yutaro, Kojima Yoshiyuki, Kohri Kenjiro
  • Organizer: American Urological Association Annual Meeting 2015
  • Place of Presentation: New Orleans（USA）
  • Year and Date: 2015-05-15
  • Int'l Joint Research
• [Presentation] Expediency of early spermatogonial stem cell activity in cryptorchid testes (2015)
  • Author(s): Hideyuki Kamisawa, Kentaro Mizuno, Satoshi Kurokawa, Yoshinobu Moritoki, Hidenori Nishio, Akihiro Nakane, Tetsuji Maruyama, Yutaro Hayashi, Kenjiro Kohri
  • Organizer: The 103rd Annual Meeting of the Japanese Urological Associations
  • Place of Presentation: 石川県立音楽堂、ANAクラウンプラザホテル金沢他(石川県金沢市)
  • Year and Date: 2015-04-18
  • Int'l Joint Research