Study on regeneration of teeth by stimulation of the third dentition

• Project/Area Number: 15K20515
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Kyoto University
• Principal Investigator: Honoka Kiso 京都大学, 医学研究科, キャリア支援診療医 (50748598)
• Research Collaborator: BESSHO Kazuhisa ; TAKAHASHI Katsu
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 歯数制御 / 歯数増加

Outline of Final Research Achievements

Some mammals has third dentition that degenerates and disappears before eruption. Tooth formed more than normal number of teeth is referred to as supernumerary tooth, and we experienced and reported cases actually thought to originate in the third dentition in humans.
In this study, we focused on the control of the number of teeth, and targeted molecules that increase the number of teeth by suppressing functions, and conversely molecules that increase the number of teeth by enhancing functions.
We reported that USAG-1 and BMP-7 may play an important role in tooth morphogenesis and size. We also reported the possibility that stem cells may be involved in the development of multiple supernumerary tooth in humans. Futhermore, analysis of CEBP / β and Runx 2 reported that epithelial mesenchymal transition enabled disengaged odontogenic epithelial stem cells to develop supernumerary teeth.

Research Products

• [Journal Article] Loss of Stemness, EMT, and Supernumerary Tooth Formation in Cebpb-/-Runx2+/- Murine Incisors. (2018)
  • Author(s): Saito K, Takahashi K, Huang B, Asahara M, Kiso H, Togo Y, Tsukamoto H, Mishima S, Nagata M, Iida M, Tokita Y, Asai M, Shimizu A, Komori T, Harada H, MacDougall M, Sugai M, Bessho K.
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 5169-5169
  • DOI: 10.1038/s41598-018-23515-y
  • NAID: 120006545187
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Two non-syndromic cases of multiple supernumerary teeth with different characteristics and a review of the literature. (2016)
  • Author(s): Katsu Takahashi, Yumiko Togo, Kazuyuki Saito, Honoka Kiso, Boyen Huang, Hiroko Tsukamoto, Kazuma Fujimura, Kazuhisa Bessho
  • Journal Title: Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology Volume: 0 Issue: 3 Pages: 00-00
  • DOI: 10.1016/j.ajoms.2015.12.006
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Antagonistic Functions of USAG-1 and RUNX2 during Tooth Development. (2016)
  • Author(s): Togo Y., Takahashi K., Saito K., Kiso H., Tsukamoto H., Huang B., Yanagita M., Sugai M., Harada H., Komori T., Shimizu A., MacDougall M., Bessho K.
  • Journal Title: PLoS One Volume: 11 Issue: 8 Pages: e0161067-e0161067
  • DOI: 10.1371/journal.pone.0161067
  • NAID: 120005842696
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of Usag-1 and Bmp7 deficiencies on murine tooth morphogenesis. (2016)
  • Author(s): Saito K., Takahashi K., Asahara M., Kiso H., Togo Y., Tsukamoto H., Huang B., Sugai M., Shimizu A., Motokawa M., Slavkin HC., Bessho K.
  • Journal Title: BMC Dev Biol. Volume: 16 Issue: 1 Pages: 14-23
  • DOI: 10.1186/s12861-016-0117-x
  • NAID: 120005819500
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dentigerous cyst associated with a supernumerary tooth in the nasal cavity: A case report (2015)
  • Author(s): Honoka Kiso, Ando Ryokoh
  • Journal Title: Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology Volume: 27 Issue: 1 Pages: 33-37
  • DOI: 10.1016/j.ajoms.2013.06.002
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 分子標的治療による欠損歯の再生: 第2 のシーズCEBP β (2018)
  • Author(s): 高橋 克、斉藤和幸、喜早ほのか、三島清香、杉並亜希子、原田英光、田畑泰彦、別所和久
  • Organizer: 第17回日本再生医療学会総会
• [Presentation] 歯の再生に向けた当科における過去5年間の過剰歯に関する検討 (2016)
  • Author(s): 喜早ほのか，高橋 克，西村隆克，小口修矢，懸野安澄，斎藤和幸，山崎 亨，浅井啓太，三島清香，別所和久
  • Organizer: 第61回（公社）日本口腔外科学会総会・学術大会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2016-11-25
• [Presentation] 歯の再生に向けたヒト非症候性多発性過剰歯の検討 (2016)
  • Author(s): 喜早ほのか，髙橋 克，小口修矢，西村隆克，斎藤和幸，三島清香，別所和久
  • Organizer: 第37回日本炎症・再生医学会
  • Place of Presentation: 京都市勧業館 みやこめっせ
  • Year and Date: 2016-06-16
• [Presentation] Interactions between BMP-7 and USAG-1 (uterine sensitization-associated gene-1) regulate supernumerary maxillary incisor formations (2015)
  • Author(s): Katsu Takahashi, Honoka Kiso, Kazuyuki Saito, Yumiko Togo, Hiroko Tsukamoto, Boyen Huang, Kazuhisa Bessho
  • Organizer: International Conference on Oral and Maxillofacial Surgery 2015
  • Place of Presentation: Melbourne Convention and Exhibition Centre
  • Year and Date: 2015-10-27
  • Int'l Joint Research
• [Presentation] 歯の再生に向けたUSAG-1とBMP-7遺伝子変異マウスにおけるエナメル上皮幹細胞の解析 (2015)
  • Author(s): 喜早ほのか、高橋 克、斎藤和幸、東郷由弥子、塚本容子、別所和久
  • Organizer: 第36回 日本炎症・再生医学会
  • Place of Presentation: 虎ノ門ヒルズフォーラム
  • Year and Date: 2015-07-21