Analysis of the chromatin status in an ultra-low scale
Project/Area Number |
15K21113
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Tumor biology
Human genetics
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Research Institution | Kyoto University |
Principal Investigator |
Tanaka Azusa 京都大学, 医学研究科, 特別研究員(PD) (70749796)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | エピゲノム / 細胞分化 / 成人T細胞白血病 / クロマチン |
Outline of Final Research Achievements |
It is now accepted that the initiation and progression of cancer, traditionally seen as a genetic disease, also involves epigenetic aberrations. Human T-cell leukemia virus type 1 (HTLV-1) is an oncogenic retrovirus and the etiological agent of adult T-cell leukemia-lymphoma (ATL). Here, we performed ATAC-seq on ATL cell samples and discovered global alterations in the chromatin status. For this experiment, we modified original ATAC-seq protocol to apply this method to limited number of cells in clinical samples. To characterize differences in chromatin accessibility in ATL cells and normal human CD4+ memory T cells, we searched for regions of gained and lost genome accessibility. Motif analysis of increased accessible sites in ATL cells identified a strong enrichment in bZIP family transcription factors. Now we are performing a knock down experiment to confirm the importance of this transcription factor.
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Report
(4 results)
Research Products
(8 results)
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[Journal Article] Myotonic dystrophy type 1 patient-derived iPSCs for the investigation of CTG repeat instability.2017
Author(s)
Ueki J, Nakamori M, Nakamura M, Nishikawa M, Yoshida Y, Tanaka A, Morizane A, Kamon M, Araki T, Takahashi MP, Watanabe A, Inagaki N, Sakurai H.
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Journal Title
Sci Rep.
Volume: 13
Issue: 1
Pages: 42522-42522
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Epigenetic Variation between Human Induced Pluripotent Stem Cell Lines Is an Indicator of Differentiation Capacity2016
Author(s)
③M. Nishizawa, K. Chonabayashi, M. Nomura, A. Tanaka, M. Nakamura, A. Inagaki, M. Nishikawa, I. Takei, A. Oishi, K. Tanabe, M. Ohnuki, H. Yokota, M. Koyanagi-Aoi, K. Okita, A. Watanabe, A. Takaori-Kondo, S. Yamanaka, Y. Yoshida
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Journal Title
Cell Stem Cell
Volume: 19
Issue: 3
Pages: 341-354
DOI
Related Report
Peer Reviewed
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[Journal Article] OVOL2 Maintains the Transcriptional Program of Human Corneal Epithelium by Suppressing Epithelial-to-Mesenchymal Transition2016
Author(s)
④K. Kitazawa, T. Hikichi, T. Nakamura, K. Mitsunaga, A. Tanaka, M. Nakamura, T. Yamakawa, S. Furukawa, M. Takasaka, N. Goshima, A. Watanabe, K. Okita, S. Kawasaki, M. Ueno, S. Kinoshita, S. Masui
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Journal Title
Cell Reports
Volume: 15
Issue: 6
Pages: 1359-1368
DOI
Related Report
Peer Reviewed / Open Access
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