Analysis of the signaling pathway of a phototropin photoreceptor in microdomain-like particles(Fostering Joint International Research)

• Project/Area Number: 15KK0254
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: SUETSUGU NORIYUKI 京都大学, 生命科学研究科, 研究員 (60514156)
• Research Collaborator: Christie John M. University of Glasgow, Institute of Molecular, Cell & Systems Biology, Professor
• Project Period (FY): 2016 – 2018
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
• Keywords: フォトトロピン / 葉緑体運動 / 青色光 / 光受容体 / 植物

Outline of Final Research Achievements

The blue light receptor phototropin regulates various light responses including phototropism, chloroplast movements, and leaf development. Two phototropin-interacting proteins RPT2 and NCH1 mediate phototropin-dependent chloroplast movements. However, how phototropins regulate these proteins remained to be determined. In this international collaborative program, I focus on the functional analysis of RPT2 and NCH1. we found that phototropins regulate blue-light-dependent dephosphorylation of NCH1. RPT2 and NCH1 interact with each other and with phototropins. Furthermore, we identified various candidate proteins that might be involved in phototropin signaling pathways.

Academic Significance and Societal Importance of the Research Achievements

フォトトロピンは植物における光合成反応の効率化を図りバイオマスの増産に必須な現象を制御する光受容体である。本研究で明らかになったフォトトロピンシグナリングの初期反応の分子メカニズムは、一つの光受容体が全く異なる現象を制御する分子機構を明らかにし植物学分野に新たな知見を与えるという学術的意義だけでなく、将来的な食糧不足の解決に不可欠である作物の効率の良いバイオマス増産法の開発研究にも重要な知見を与えるという社会的意義も持つ。

Research Products

• [Int'l Joint Research] グラスゴー大学(英国) (2016)
  • Year and Date: 2016-11-01
• [Journal Article] Chloroplast Accumulation Response Enhances Leaf Photosynthesis and Plant Biomass Production (2018)
  • Author(s): Gotoh Eiji、Suetsugu Noriyuki、Yamori Wataru、Ishishita Kazuhiro、Kiyabu Ryota、Fukuda Masako、Higa Takeshi、Shirouchi Bungo、Wada Masamitsu
  • Journal Title: Plant Physiology Volume: 178 Issue: 3 Pages: 1358-1369
  • DOI: 10.1104/pp.18.00484
  • Peer Reviewed / Open Access
• [Journal Article] Shining light on the function of NPH3/RPT2-Like (NRL) proteins. (2018)
  • Author(s): Christie J.M., Suetsugu, N., Sullivan S., and Wada M.
  • Journal Title: Plant Physiology Volume: 176 Issue: 2 Pages: 1015-1024
  • DOI: 10.1104/pp.17.00835
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Palisade cell shape affects the light-induced chloroplast movements and leaf photosynthesis (2018)
  • Author(s): Gotoh, E., N. Suetsugu, T. Higa, T. Matsushita, H. Tsukaya and M. Wada
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 1472-1472
  • DOI: 10.1038/s41598-018-19896-9
  • NAID: 120006394740
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome. (2017)
  • Author(s): Bowman J, Kouchi T,..(106名）..Yokoyama R, ..(4名)
  • Journal Title: Cell Volume: 171 Issue: 2 Pages: 287-304
  • DOI: 10.1016/j.cell.2017.09.030
  • NAID: 120006353839
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Two coiled-coil proteins, WEB1 and PMI2, suppress the signaling pathway of chloroplast accumulation response that mediated by two phototropin-interacting proteins, RPT2 and NCH1, in seed plants. (2017)
  • Author(s): Suetsugu, N., and M.Wada
  • Journal Title: International Journal of Molecular Sciences, Volume: 18 Issue: 7 Pages: 1469-1469
  • DOI: 10.3390/ijms18071469
  • Peer Reviewed / Open Access
• [Journal Article] Ferns, mosses, and liverworts as model systems for light-mediated chloroplast movements. (2017)
  • Author(s): Suetsugu T., T. Higa, and M. Wada
  • Journal Title: Plant Cell Environment Volume: 印刷中 Issue: 11 Pages: 2447-2456
  • DOI: 10.1111/pce.12867
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] An adenylyl cyclase with a phosphodiesterase domain in basal plants with a motile sperm system. (2016)
  • Author(s): Kasahara, M., Suetsugu, N., Urano, Y., Yamamoto, C., Ohmori, M., Takada, Y., Okuda, S., Nishiyama, T., Sakayama, H., Kohchi, T., Takahashi, F.
  • Journal Title: Sci. Rep. Volume: 6 Issue: 1 Pages: 39232-39232
  • DOI: 10.1038/srep39232
  • Peer Reviewed / Open Access
• [Presentation] NCH1 is a key factor that specifically mediates phototropin-dependent chloroplast accumulation movement in land plants (2018)
  • Author(s): Suetsugu N, Sullivan S, Kohchi T, Christie JM.
  • Organizer: International Symposium on Plant Photobiology, 2018
  • Int'l Joint Research
• [Presentation] NCH1 is a key factor that specifically mediates phototoropin-dependent chloroplast accumulation movement in land plants (2018)
  • Author(s): Noriyuki Suetsugu, Stuart Sullivan, Takayuki Kohchi, John M. Christie
  • Organizer: International Symposium on Plant Photobiology, 2018
  • Int'l Joint Research
• [Presentation] RPT2 and NCH1 are key factors for the blue-light receptor phototoropin-signaling pathway in land plants (2017)
  • Author(s): Noriyuki Suetsugu, Stuart Sullivan, Takayuki Kohchi, John M. Christie
  • Organizer: From Proteome to Phenotype: role of post-translational modifications(The Society for Experimental BIology)
  • Int'l Joint Research
• [Presentation] 光による葉緑体定位運動の分子メカニズムの研究 (2016)
  • Author(s): 末次 憲之
  • Organizer: 日本植物学会第80回大会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県・宜野湾市）
  • Year and Date: 2016-09-16
  • Invited