Characterization of exosomes released from Epstein-Barr virus-infected cells(Fostering Joint International Research)
| Project/Area Number |
15KK0324
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| Research Category |
Fund for the Promotion of Joint International Research (Fostering Joint International Research)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | Hokkaido University |
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Principal Investigator |
Nanbo Asuka 北海道大学, 医学研究院, 准教授 (60359487)
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| Research Collaborator |
Sugden Bill ウィスコンシン大学マディソン校, マッカードル癌研究所, 教授
OHBA Yusuke
YOSHIYAMA Hironori
KATANO Harutaka
NODA Takeshi
OHASHI Makoto
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| Project Period (FY) |
2016 – 2018
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
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| Keywords | Epstein-Barrウイルス / エキソソーム / microRNA / EBV関連がん / miRNA / 次世代シーケンス / Epstein-Barr virus / マイクロRNA |
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| Outline of Final Research Achievements |
Infection of Epstein-Barr virus (EBV), a ubiquitous human herpesvirus, is associated with various malignancies. EBV-infected cells have been shown to secrete extracellular micro vesicles, exosomes, which possess various functions by transferring sets of proteins, lipids and RNAs to the recipient cells.
Although accumulating evidence demonstrates that exosomes released form EBV-infected cells transfer viral factors including microRNAs to recipient cells, little is known about their roles in vital lifecycle. Here, we found that the biogenesis of exosomes is upregulated in infected cells. We also observed that several specific microRNAs were predominantly incorporated in the exosomes released from infected cells, potentially contributing to phenotypic changes in cells receiving these exosomes.
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| Academic Significance and Societal Importance of the Research Achievements |
近年、がん細胞から放出されたエキソソームが、特定のマイクロRNA を標的細胞に供給することで、がんの増殖や転移に関与することが報告されつつある。また，特定のマイクロRNA ががんの早期段階で発現し、エキソソームを介して血液中に放出される現象を利用し、血液中のマイクロRNA の量を測定することで、がんの早期発見や診断法に応用できるものと期待されている。今後、本研究を発展させることで、EBV関連がん発症メカニズムの詳細な解明につながることが期待される。また、本研究で同定された、エキソソームに濃縮される特定のマイクロRNA を対象として、EBV関連がんの診断法の実現につながることが期待される。
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] A Peptide Derived from Phosphoinositide 3-kinase Inhibits Endocytosis and Influenza Virus Infection2019
Author(s)
Fujioka Y, Satoh AO, Horiuchi K, Fujioka M, Tsutsumi K, Sasaki J, Nepal P, Kashiwagi S, Paudel S, Nishide S, Nanbo A, Sasaki T, Ohba Y
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Journal Title
Cell Structure and Function
Volume: 44
Issue: 1
Pages: 61-74
DOI
NAID
ISSN
0386-7196, 1347-3700
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A Sialylated Voltage-Dependent Ca2+ Channel Binds Hemagglutinin and Mediates Influenza A Virus Entry into Mammalian Cells2018
Author(s)
Fujioka Y, Nishide S, Ose T, Suzuki T, Kato I, Fukuhara H, Fujioka M, Horiuchi K, Satoh AO, Nepal P, Kashiwagi S, Wang J, Horiguchi M, Sato Y, Paudel S, Nanbo A, Miyazaki T, Hasegawa H, Maenaka K, Ohba Y
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Journal Title
Cell Host Microbe
Volume: 23
Issue: 6
Pages: 809-818
DOI
NAID
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Improved FRET Biosensor for the Measurement of BCR-ABL Activity in Chronic Myeloid Leukemia Cells2017
Author(s)
M. Horiguchi, M. Fujioka, T. Kondo, Y. Fujioka, X. Li, K. Horiuchi, A.O. Satoh, P. Nepal, S. Nishide, A. Nanbo, T. Teshima and Y. Ohba.
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Journal Title
Cell Structure and Function
Volume: 42
Issue: 1
Pages: 15-26
DOI
NAID
ISSN
0386-7196, 1347-3700
Related Report
Peer Reviewed / Open Access
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[Journal Article] Fcγ-receptor IIa-mediated Src Signaling Pathway Is Essential for the Antibody-Dependent Enhancement of Ebola Virus Infection.2016
Author(s)
Furuyama W, Marzi A, Carmody AB, Maruyama J, Kuroda M, Miyamoto H, Nanbo A, Manzoor R, Yoshida R, Igarashi M, Feldmann H, Takada A.
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Journal Title
PLoS Pathog.
Volume: 12(12)
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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