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Mechanism of apical-basal polarity formation in epithelial cells

Research Project

Project/Area Number 15KT0086
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeMulti-year Fund
Section特設分野
Research Field Constructive Systems Biology
Research InstitutionThe University of Tokushima

Principal Investigator

YONEMURA Shigenobu  徳島大学, 大学院医歯薬学研究部(医学系), 教授 (60192811)

Co-Investigator(Kenkyū-buntansha) 柴田 達夫  国立研究開発法人理化学研究所, 生命システム研究センター, チームリーダー (10359888)
Project Period (FY) 2015-07-10 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Keywords上皮細胞 / 上皮極性 / 細胞間接着 / 定量生物学 / 細胞外基質 / 網羅的スクリーニング / RNAi / 細胞間接着装置 / ゲノムワイドスクリーニング / 数理解析 / ミオシン / 画像解析 / siRNAスクリーニング / 上皮 / 極性
Outline of Final Research Achievements

Epithelial cell sheets form compartments that discriminate outer and inner environments and the apical-basal polarity of the epithelial cell is essential for the function. The mechanism of the formation and maintenance of the polarity, however, has not been fully understood. We made a basis for constructing a model that explains how the polarity is formed by quantitative measurements of protein amount along the cell cortex under normal or experimental conditions. Models containing the role of cell-cell and cell-substrate adhesions are under consideration. We also tried to identify important genes for polarity formation from genom-wide screening. After development of effective methods for measurement and analysis, we performed RNAi screening using more than 15,000 human genes. The results contained not only genes already reported but also new genes which involvement appears to be intriguing.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (25 results)

All 2018 2017 2016 2015 Other

All Journal Article (17 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 15 results,  Open Access: 15 results,  Acknowledgement Compliant: 2 results) Presentation (7 results) (of which Int'l Joint Research: 1 results,  Invited: 4 results) Remarks (1 results)

  • [Journal Article] Neural retina-specific Aldh1a1 controls dorsal choroidal vascular development via Sox9 expression in retinal pigment epithelial cells.2018

    • Author(s)
      Goto S, Onishi A, Misaki K, Yonemura S, Sugita S, Ito H, Ohigashi Y, Ema M, Sakaguchi H, Nishida K, Takahashi M.
    • Journal Title

      eLife

      Volume: 7

    • DOI

      10.7554/elife.32358

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The force‐sensing device region of α‐catenin is an intrinsically disordered segment in the absence of intramolecular stabilization of the autoinhibitory form2018

    • Author(s)
      Hirano, Y., Amano, Y., Yonemura, S. and Hakoshima, T.
    • Journal Title

      Genes to Cells

      Volume: 23 Issue: 5 Pages: 16-16

    • DOI

      10.1111/gtc.12578

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Establishment of Immunodeficient Retinal Degeneration Model Mice and Functional Maturation of Human ESC-Derived Retinal Sheets after Transplantation2018

    • Author(s)
      Iraha Satoshi、Tu Hung-Ya、Yamasaki Suguru、Kagawa Takahiro、Goto Motohito、Takahashi Riichi、Watanabe Takehito、Sugita Sunao、Yonemura Shigenobu、Sunagawa Genshiro A.、Matsuyama Take、Fujii Momo、Kuwahara Atsushi、Kishino Akiyoshi、Koide Naoshi、Eiraku Mototsugu、Tanihara Hidenobu、Takahashi Masayo、Mandai Michiko
    • Journal Title

      Stem Cell Reports

      Volume: 10 Issue: 3 Pages: 1059-1074

    • DOI

      10.1016/j.stemcr.2018.01.032

    • NAID

      120007037461

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Real-time TIRF observation of vinculin recruitment to stretched α-catenin by AFM.2018

    • Author(s)
      Maki, K., Han, S. -U., Hirano, Y., Yonemura, S. Hakoshima, T. and Adachi, T.
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 1575-1575

    • DOI

      10.1038/s41598-018-20115-8

    • NAID

      120006517895

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Androgen Regulates Dimorphic F-Actin Assemblies in the Genital Organogenesis2017

    • Author(s)
      Liu Liqing、Suzuki Kentaro、Chun Eunice、Murashima Aki、Sato Yuki、Nakagata Naomi、Fujimori Toshihiko、Yonemura Shigenobu、He Wanzhong、Yamada Gen
    • Journal Title

      Sexual Development

      Volume: 11 Issue: 4 Pages: 190-202

    • DOI

      10.1159/000477452

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Medaka and zebrafish contactin1 mutants as a model for understanding neural circuits for motor coordination2017

    • Author(s)
      Takeuchi Miki、Inoue Chikako、Goshima Akiko、Nagao Yusuke、Shimizu Koichi、Miyamoto Hiroki、Shimizu Takashi、Hashimoto Hisashi、Yonemura Shigenobu、Kawahara Atsuo、Hirata Yutaka、Yoshida Masayuki、Hibi Masahiko
    • Journal Title

      Genes to Cells

      Volume: 22 Issue: 8 Pages: 723-741

    • DOI

      10.1111/gtc.12509

    • NAID

      120006367981

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] SHG-specificity of cellular Rootletin filaments enables naive imaging with universal conservation2017

    • Author(s)
      Toshihiro Akiyama, Akihito Inoko, Yuichi Kaji, Shigenobu Yonemura, Kisa Kakiguchi, Hiroki Segawa, Kei Ishitsuka, Masaki Yoshida, Osamu Numata, Philippe Leproux, Vincent Couderc, Tetsuro Oshika, Hideaki Kano
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 39967-39967

    • DOI

      10.1038/srep39967

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Actin filament association at adherens junctions2017

    • Author(s)
      S. Yonemura.
    • Journal Title

      The Journal of Medical Investigation

      Volume: 64 Issue: 1.2 Pages: 14-19

    • DOI

      10.2152/jmi.64.14

    • NAID

      130005501948

    • ISSN
      1343-1420, 1349-6867
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inheritance of a Nuclear PIWI from Pluripotent Stem Cells by Somatic Descendants Ensures Differentiation by Silencing Transposons in Planarian.2016

    • Author(s)
      Shibata N, Kashima M, Ishiko T, Nishimura O, Rouhana L, Misaki K, Yonemura S, Saito K, Siomi H, Siomi MC, Agata K.
    • Journal Title

      Developmental Cell

      Volume: 37 Issue: 3 Pages: 226-237

    • DOI

      10.1016/j.devcel.2016.04.009

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mechano-adaptive sensory mechanism of α-catenin under tension2016

    • Author(s)
      Koichiro Maki, Sung-Woong Han, Yoshinori Hirano, Shigenobu Yonemura, Toshio Hakoshima & Taiji Adachi
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 24878-24878

    • DOI

      10.1038/srep24878

    • NAID

      120006337976

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Single microfilaments mediate the early steps of microtubule bundling during preprophase band formation in onion cotyledon epidermal cells2016

    • Author(s)
      Takeuchi M, Karahara I, Kajimura N, Takaoka A, Murata K, Misaki K, Yonemura S, Staehelin LA, Mineyuki Y.
    • Journal Title

      Mol Biol Cell.

      Volume: 27 Issue: 11 Pages: 1809-1820

    • DOI

      10.1091/mbc.e15-12-0820

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] IKKε inhibits PKC to promote Fascin-dependent actin 2016bundling2016

    • Author(s)
      Otani T, Ogura Y, Misaki K, Maeda T, Kimpara A, Yonemura S, Hayashi S
    • Journal Title

      Development

      Volume: 143 Issue: 20 Pages: 3806-3816

    • DOI

      10.1242/dev.138495

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.2016

    • Author(s)
      Inaba H, Goto H, Kasahara K, Kumamoto K, Yonemura S, Inoko A, Yamano S, Wanibuchi H, He D, Goshima N, Kiyono T, Hirotsune S, Inagaki M.
    • Journal Title

      J Cell Biol.

      Volume: 212 Issue: 4 Pages: 409-423

    • DOI

      10.1083/jcb.201507046

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells.2016

    • Author(s)
      Ozone C, Suga H, Eiraku M, Kadoshima T, Yonemura S, Takata N, Oiso Y, Tsuji T, Sasai Y.
    • Journal Title

      Nat Commun.

      Volume: 7 Issue: 1 Pages: 10351-10351

    • DOI

      10.1038/ncomms10351

    • NAID

      120006535047

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transplantation of human embryonic stem cell-derived retinal tissue in two primate models of retinal degeneration.2016

    • Author(s)
      Shirai H, Mandai M, Matsushita K, Kuwahara A, Yonemura S, Nakano T, Assawachananont J, Kimura T, Saito K, Terasaki H, Eiraku M, Sasai Y, Takahashi M.
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 113 Issue: 1

    • DOI

      10.1073/pnas.1512590113

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 細胞間メカノトランスダクション2016

    • Author(s)
      鳥澤久美子, 米村重信
    • Journal Title

      生体の科学

      Volume: 67 Pages: 137-141

    • Related Report
      2015 Research-status Report
  • [Journal Article] Absence of Radial Spokes in Mouse Node Cilia Is Required for Rotational Movement but Confers Ultrastructural Instability as a Trade-Off.2015

    • Author(s)
      Shinohara K, Chen D, Nishida T, Misaki K, Yonemura S, Hamada H.
    • Journal Title

      Dev Cell.

      Volume: 35 Pages: 236-246

    • Related Report
      2015 Research-status Report
  • [Presentation] αカテニンのアクチン結合能の制御2017

    • Author(s)
      米村重信、林華子、上田太郎
    • Organizer
      第2回 日本メカノバイオロジー学会
    • Place of Presentation
      京都大学農学部 京都府 京都市
    • Year and Date
      2017-03-15
    • Related Report
      2016 Research-status Report
  • [Presentation] 細胞間接着と上皮形態形成2017

    • Author(s)
      米村重信
    • Organizer
      日本細胞生物学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 互いに接着しない上皮細胞を用いた上皮極性因子の ゲノムワイドRNAiスクリーニング2017

    • Author(s)
      本田尚三、名黒功、米村重信
    • Organizer
      日本メカノバイオロジー学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 上皮細胞が丸くなれる仕組み -細胞間接着装置におけるメカノトランスダクション-2016

    • Author(s)
      米村重信
    • Organizer
      平成 28 年度公益社団法人日本顕微鏡学会 北海道支部学術講演会
    • Place of Presentation
      北海道大学工学部 北海道 札幌市
    • Year and Date
      2016-12-10
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Force-sensing device in alpha-catenin,2016

    • Author(s)
      Shigenobu Yonemura
    • Organizer
      “Mechanobiology, from molecules to tissue” ICISE
    • Place of Presentation
      ICISE, Quy Nhon, Viet Nam
    • Year and Date
      2016-06-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] お互い接着しない上皮細胞を使って極性形成機構を探求する2016

    • Author(s)
      米村重信、本田尚三
    • Organizer
      第68回日本細胞生物学会大会
    • Place of Presentation
      京都メルサ 京都府 京都市
    • Year and Date
      2016-06-17
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] お互い接着しない上皮細胞を使って極性形成機構を探求する2016

    • Author(s)
      米村重信
    • Organizer
      日本細胞生物学会大会
    • Place of Presentation
      国内 京都テルサ 京都市
    • Year and Date
      2016-06-17
    • Related Report
      2015 Research-status Report
    • Invited
  • [Remarks] 徳島大学大学院 医歯薬学研究部 医科学部門 生理系 細胞生物学分野

    • URL

      http://oba.to/yonemura/index.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-07-14   Modified: 2019-03-29  

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