| Budget Amount *help |
¥601,900,000 (Direct Cost: ¥463,000,000、Indirect Cost: ¥138,900,000)
Fiscal Year 2008: ¥64,740,000 (Direct Cost: ¥49,800,000、Indirect Cost: ¥14,940,000)
Fiscal Year 2007: ¥107,770,000 (Direct Cost: ¥82,900,000、Indirect Cost: ¥24,870,000)
Fiscal Year 2006: ¥133,770,000 (Direct Cost: ¥102,900,000、Indirect Cost: ¥30,870,000)
Fiscal Year 2005: ¥147,810,000 (Direct Cost: ¥113,700,000、Indirect Cost: ¥34,110,000)
Fiscal Year 2004: ¥147,810,000 (Direct Cost: ¥113,700,000、Indirect Cost: ¥34,110,000)
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| Research Abstract |
Using two-photon microscope and uncaging of caged-glutamate compounds, we have revealed that rapid motilities of dendritic spines underlie plasticity of excitatory synaptic transmission in the cerebral cortex. We have visualized how actin reorganization underlies the motilities, and how spine sizes can be maintained over days even though the synapses are living small structures, and cannot avoid the natural fluctuations. We have developed a new caged-GABA compound, which can be used simultaneously with caged-glutamate compound. A new principle for visualization of exocytosis with two-photon microscope has been established, and applied to analyze conformational changes of responsible molecules.
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