| Budget Amount *help |
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2005: ¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 2004: ¥7,500,000 (Direct Cost: ¥7,500,000)
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| Research Abstract |
Pathogenic agents of prion diseases, namely prions, have diverse strains each of which results in a characteristic clinical course and pathology in the inoculated mice. A previous study suggested that the mice infected with some prion strains were resistant against subsequent infection with other strains. In order to clarify the mechanisms, we examined whether the interference between prion strains were reproduced in the cell culture models. The mouse neuronal cells, GT1-7, persistently infected with a scrapie-derived mouse-adapted Chandler strain were permissive, but those with 22L strain were resistant against superinfection with a CJD-derived mouse-adapted strain, Fukuoka-1, indicating that 22L interferes Fukuoka-1 in neuronal cells without involvements of immune cells, Moreover, we demonstrated that an attenuated CJD strain, SY, successfully interfered subsequent challenges with virulent strains, Chandler, 22L, and Fukuoka-1. The findings have opened novel prophylactic and therapeutic strategies by the interference.
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