Study of cell morphogenesis that is coupled with cell division cycle

• Project/Area Number: 16026205
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: OHYA Yoshikazu The University of Tokyo, Graduate School of Frontier Sciences, Department of Integrated Biosciences, Professor, 大学院新領域創成科学研究科, 教授 (20183767)
• Project Period (FY): 2004 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥23,100,000 (Direct Cost: ¥23,100,000); Fiscal Year 2005: ¥11,700,000 (Direct Cost: ¥11,700,000); Fiscal Year 2004: ¥11,400,000 (Direct Cost: ¥11,400,000)
• Keywords: cell division cycle / Saccharomyces cerevisiae / cell wall / checkpoint / dynactin / cell morphology

Research Abstract

The coordination of cell cycle events during cell proliferation is attained, in part, through surveillance systems. In the budding yeast Saccharomyces cerevisiae, cell cycle progression is restrictively regulated and some events during cell cycle are regulated by the checkpoints monitoring DNA damage, DNA replication, integrity of the spindle, spindle positioning and cell morphogenesis. It still remained unclear whether all of the checkpoint controls have been identified.
We found that a novel checkpoint exists to couple cell wall remodeling with spindle formation. fksl mutants defective in 1,3-β-glucan synthase activity failed to form a mature bud. Surprisingly, these cells arrested with post-replicative DNA but quite low level of C1b2p, and without forming bipolar spindles. We reported that wadi (wall-checkpoint defective) mutation that abolished this arrest caused the accumulation of C1b2p and CLB2 mRNA and leaded to formation of bipolar spindles despite glucan-synthesis perturbation. These results indicate the existence of a novel checkpoint mechanism to coordinate entry into mitosis, and suggest that Wac1 p is required to achieve this checkpoint function through a transcriptional regulation of CLB2. Furthermore, we revealed that WACI was identical with ARP1 (actin-related protein). Arp1p, Nip100p and Jnm1p, which are components of the dynactin complex, are required to achieve the G2 arrest while keeping cells highly viable. These results indicate that the dynactin complex have a regulatory role in the novel checkpoint to monitor cell wall synthesis.

Research Products

• [Journal Article] Evaluation of image processing programs for accurate measurement of budding and fission yeast morphology. (2006)
  • Author(s): Suzuki, G. et al.
  • Journal Title: Curr. Genet 49(4) Pages: 237-247
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Evaluation of image processing programs for accurate measurement of budding and fission yeast morphology. (2006)
  • Author(s): Suzuki, G., Sawai, H., Ohtani, M., Nogami, S., Sano-Kumagai, F., Saka, A., Yukawa, M., Saito, T.L., Sese, J., Hirata, D., Morishita, S., Ohya Y.
  • Journal Title: Curr. Genet. 49(4) Pages: 237-247
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Evaluation of image processing programs for accurate measurement of budding and fission yeast morphology (2006)
  • Author(s): Suzuki, G
  • Journal Title: Curr Genet. 49 Pages: 237-247
• [Journal Article] High-dimensional and large-scale phenotyping of yeast mutants. (2005)
  • Author(s): Ohya, Y. et al.
  • Journal Title: Proc. Natl. Acad. Sci. USA 102(52) Pages: 19015-19020
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Piperazine propanol derivative as a novel antifungal targeting 1,3-beta-D-glucan synthase. (2005)
  • Author(s): Kondoh, O. et al.
  • Journal Title: Biol Pharm Bull. 28(11) Pages: 2138-2141
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Data Mining Tools for Saccharomyces Cerevisiae Morphological Database. (2005)
  • Author(s): Saito, T. et al.
  • Journal Title: Nuc. Acid Res. 33
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular dissection of ARPI regions required for nuclear migration and cell wall integrity checkpoint functions in Saccharomyces cerevisiae. (2005)
  • Author(s): Igarashi, T. et al.
  • Journal Title: Cell Struct Funct. 30(2) Pages: 57-67
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Involvement of actin and polarisome in morphological change during spore germination of Saccharomyces cerevisiae. (2005)
  • Author(s): Kono, K. et al.
  • Journal Title: Yeast 22(2) Pages: 129-139
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs : Implications for the molecular pathogenesis of human mitochondrial diseases. (2005)
  • Author(s): Umeda, N. et al.
  • Journal Title: J Biol Chem. 280(2) Pages: 1613-1624
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] High-dimensional and large-scale phenotyping of yeast mutants. (2005)
  • Author(s): Ohya, Y., Sese, J., Yukawa, M., Sano, F., Nakatani, Y., Saito, TL., Saka.A., Fukuda, T., Ishihara, S., Oka, S., Suzuki, G., Watanabe, M., Hirata, A., Ohtani, M., Sawai, H., Fraysse, N., Latge, JP., Francois, JM., Aebi, M., Tanaka, S., Muramatsu, S., Araki, H., Sonoike, K., Nogami, S., Morishita, S.
  • Journal Title: Proc Natl Acad Sci USA. Dec 27;102(52)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Piperazine propanol derivative as a novel antifungal targeting 1,3-beta-D-glucan synthase. (2005)
  • Author(s): Kondoh, 0., Inagaki, Y., Fukuda, H., Mizuguchi, E., Ohya, Y., Arisawa, M., Shimma, N., Aoki, Y., Sakaitani, M., Watanabe, T.
  • Journal Title: Biol Pharm Bull. Nov 28(11) Pages: 2138-41
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Data Mining Tools for Saccharomyces Cerevisiae Morphological Database. (2005)
  • Author(s): Saito, T., Sese, J., Nakatani, Y., Sano F., Yukawa, M., Ohya, Y., Morishita S.
  • Journal Title: Nuc. Acid Res. Jul 1;33(Web Server issue)
  • NAID: 120006811259
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular dissection of ARP1 regions required for nuclear migration and cell wall integrity checkpoint functions in Saccharomyces cerevisiae. (2005)
  • Author(s): Igarashi R, Suzuki M, Nogami S, Ohya Y.
  • Journal Title: Cell Struct Fund. 30(2) Pages: 57-67
  • NAID: 130004137499
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Involvement of actin and polarisome in morphological change during spore germination of Saccharomyces cerevisiae. (2005)
  • Author(s): Kono, K., Matsunaga, R1, Hirata, A., Suzuki, -G., Abe, M., Ohya, Y.
  • Journal Title: Yeast Jan 30;22(2) Pages: 129-39
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs : Implications for the molecular pathogenesis of human mitochondrial diseases. (2005)
  • Author(s): Umeda, N., Suzuki, T., Yukawa, M., Ohya, Y., Shindo, H., Watanabe, K., Suzuki, T.
  • Journal Title: J. Biol. Chem. Jan 14;280(2)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] High-dimensional and large-scale phenotyping of yeast mutants (2005)
  • Author(s): Ohya, Y.
  • Journal Title: Proc.Natl.Acad.Sci.USA 102 Pages: 19015-19020
• [Journal Article] Molecular dissection of ARP1 regions required for nuclear migration and cell wall integrity checkpoint functions in Saccharomyces cerevisiae (2005)
  • Author(s): Igarashi, R
  • Journal Title: Cell Struct Funct 30 Pages: 57-67
• [Journal Article] Recruitment of RecA homologs Dmc1p and Rad51p to the double-strand break repair site initiated by meiosis-specific endonuclease VDE(PI-ScaI). (2005)
  • Author(s): Fukuda, T.
  • Journal Title: Mol Genet Genomics 275 Pages: 204-214
• [Journal Article] nvolvement of actin and polarisome in morphological change during spore germination of Saccharomyces cerevisiae. (2005)
  • Author(s): Kono, K
  • Journal Title: Yeast 30 Pages: 129-139
• [Journal Article] Hox code in embryos of Chinese soft-shelled turtle Pelodiscus sinensis correlates with the evolutionary innovation in the turtle. (2005)
  • Author(s): Umeda
  • Journal Title: J Biol Chem. 280 Pages: 1613-1924
• [Journal Article] Dynactin complex is involved in a checkpoint to monitor cell wall synthesis in Saccharomyces cerevisiae. (2004)
  • Author(s): Suzuki, M. et al.
  • Journal Title: Nat. Cell Biol. 6 Pages: 861-871
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A novel mechanism of intragenic complementation between Phe to Ala calmodulin mutations. (2004)
  • Author(s): Okano, H., Ohya, Y.
  • Journal Title: J. Biochem. 135 Pages: 289-295
  • NAID: 10016198578
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cell shape and growth of budding yeast cells in restrictive microenvironments. (2004)
  • Author(s): Suzuki, M. et al.
  • Journal Title: Yeast 21 Pages: 983-989
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular mechanism of vde-initiated intein homing in yeast nuclear genome. (2004)
  • Author(s): Fukuda T, et al.
  • Journal Title: Adv Biophys. 38 Pages: 215-232
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] SCMD : Saccaromyces cerevisiae morphological database. (2004)
  • Author(s): Saito, T.L.et al.
  • Journal Title: Nuc. Acid Res. 32
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Analysis of the molecular interaction of the farnesyl moiety of transducin through use of a photoreactive farnesyl analog. (2004)
  • Author(s): Hagiwara, K. et al.
  • Journal Title: Biochemstry 43 Pages: 300-309
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Development of Image Processing Program for Yeast Cell Morphology. (2004)
  • Author(s): Ohtani, M. et al.
  • Journal Title: J. Bioinfo. Comput. Biol. 1 Pages: 695-709
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Dynactin complex is involved in a checkpoint to monitor cell wall synthesis in Saccharomyces cerevisiae. (2004)
  • Author(s): Suzuki, M., Igarashi, R., Sekiya, M., Utsugi, T., Morishita, S., Yukawa, M., Ohya, Y.
  • Journal Title: Nat. Cell Biol. 6 Pages: 861-71
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cell shape and growth of budding yeast cells in restrictive microenvironments. (2004)
  • Author(s): Suzuki, M., Asada, Y., Watanabe, D., Ohya Y.
  • Journal Title: Yeast 21 Pages: 983-9
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular mechanism of vde-initiated intein homing in yeast nuclear genome. (2004)
  • Author(s): Fukuda T, Nagai Y, Ohya Y.
  • Journal Title: Adv Biophys. 38 Pages: 215-232
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Saccaromyces cerevisiae morphological database. (2004)
  • Author(s): Saito, T.L., Ohtani, M., Sawai, H., Sano, F., Saka, A., Watanabe, D., Yukawa, M., Ohya, Y., Morishita, S.: SCMD
  • Journal Title: Nuc. Acid Res. 32
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Analysis of the molecular interaction of the famesyl moiety of transducin through use of a photoreactive famesyl analog. (2004)
  • Author(s): Hagiwara, K., Wada, A., Katadae, M., Ito, M., Ohya, Y., Casey, P.J., Fukada, Y.
  • Journal Title: Biochemstry 43 Pages: 300-309
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Development of Image Processing Program for Yeast Cell Morphology. (2004)
  • Author(s): Ohtani, M., Sano, F., Saka, A., Ohya, Y., Morishita, S.
  • Journal Title: J. Bioinfo. Comput. Biol. 1 Pages: 695-709
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cell shape and growth of budding yeast cells in restrictive microenvironments. (2004)
  • Author(s): Suzuki, M., Asada, Y., Watanabe, D., Ohya, Y.
  • Journal Title: Yeast 21 Pages: 983-989
• [Journal Article] Dynactin complex is involved in a checkpoint to monitor cell wall synthesis in Saccharomyces cerevisiae. (2004)
  • Author(s): Suzuki, M., Igarashi, R., Sekiya, M., Utsugi, T., Morishita, S., Yukawa, M., Ohya, Y
  • Journal Title: Nat.Cell Biol 6 Pages: 861-871