| Project/Area Number |
16074217
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SHIRO Yoshitsugu The Institute of Physical and Chemical Research, SPring-8 Center, Chief Scientist (70183051)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIMOTO Hiroshi RIKEN SPring-8 Centerr, 城生体金属科学研究室, Research Scientist (90344043)
NAGANO Shingo RIKEN SPring-8 Center, 城生体金属科学研究室, Senior Research Scientist (60286440)
山田 斉爾 独立行政法人理化学研究所, 城生体金属科学研究室, 基礎科学特別研究員 (20360662)
日野 智也 独立行政法人理化学研究所, 城生体金属科学研究室, 協力研究員 (40373360)
汲田 英之 独立行政法人理化学研究所, 城生体金属科学研究室, 協力研究員 (70332286)
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| Project Period (FY) |
2004 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 2007: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2006: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Metalloproteins / Bio-synthesis / Signal Transduction / Metal-enzymes / coordination Chemistry / ヘム鉄 / 二原子酸素添加酵素 / 二成分情報伝達系 / センサー蛋白質 / 一酸化窒素還元酵素 / X線結晶構造解析 / 配位構造 / ヘム / トリプトファン代謝 / キヌレニン経路 / 老人性白内障 / 感染症 / 金属配位空間 / ヒスチジンキナーゼ / 酵素センサー蛋白質 / エチレンセンサー蛋白質 / 一酸化窒素 / 脱窒 / 非ヘム鉄 |
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| Research Abstract |
We have examined conversion of bio-substances and information in molecular level by metal-containing enzymes and proteins such as nitric oxide reductase, indoleamine 2, 3-dioxygenase, and oxygen sensor protein. We proposed the reaction mechansim of conversion of NO (nitric oxide) to N20 (nitrous oxide) on the basis of the ESR results of the reaction intermediates trapped by the rapid mixing-to-quench system, and the crystal structure of this enzyme. We also proposed the reaction mechanism of the tryptophan metabolism catalyzed by indoleamine 2,3-dioxygenase on the basis of its crystal structure. We found that the oxygen sensor protein can descrominate 02 from other two-atomic gas molecules such as CO and NO, and also discussed the pathway of the intra- and inter-molecular signal transduction on the basis of the strcuture of the sensor protein of the thermophilic bacterium, which is analogus to the oxygen sensor protein.
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