Development of fundamental basis for embryo-biotechnology in next generation by clarifying the regulatory mechanisms of cell differentiation and apoptosis of oocytes

• Project/Area Number: 16108003
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Tohoku University
• Principal Investigator: SATO Eimei Tohoku University, 大学院・農学研究科, 教授 (80093243)
• Co-Investigator(Kenkyū-buntansha): NISHIMORI Katuhiko 東北大学, 大学院・農学研究科, 教授 (10164609) ; TAKEYA Tatuo 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (60112330) ; MANABE Noboru 東京大学, 大学院・農学生命科学研究科, 教授 (80243070) ; HOSHINO Yumi 東北大学, 大学院・農学研究科, 助教 (10451551) ; 佐々田 比呂志 東北大学, 大学院農学研究科, 助教授 (90158931) ; 松本 浩道 東北大学, 大学院・農学研究科, 助手 (70241552)
• Project Period (FY): 2004 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥104,520,000 (Direct Cost: ¥80,400,000、Indirect Cost: ¥24,120,000); Fiscal Year 2008: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000); Fiscal Year 2007: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000); Fiscal Year 2006: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000); Fiscal Year 2005: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000); Fiscal Year 2004: ¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
• Keywords: 受精 / 体細胞初期化 / 卵巣特異的遺伝子 / 卵母細胞の死滅 / 卵母細胞の生存促進 / 卵子 / 卵胞 / ヒアルロン酸 / CD44 / マクロファージ / VEGF / GDF-9 / マウス / ミニブタ体細胞クローン / 遺伝子改変マウス / Cx43 / アポトーシス / Oct4 / 受精能 / 卵胞発育 / 卵胞閉鎖 / CD44 antigen glycosylation / 未成熟卵子凍結保存法(SWEID) / Connexin 43 / TAP発現ベクター / GDF-9遺伝子 / 体細胞初期化能 / ミトコンドリア置換 / FLIP / iRNA法 / ES細胞 / ギャップ結合構成コネクシン遺伝子

Research Abstract

1個体からの受精可能卵子の大量生産を目標として卵子の細胞分化・死滅の調節系の解明を行い、新規調節因子を同定するとともに、これを踏まえ受精能・体細胞初期化能高発現卵子生産などの技術を開発した。さらに、直径70μm未満のマウス卵胞卵子由来の産子や家畜ブタ体外成熟卵子をレシピエントとする体細胞ミニブタ作出に成功した。

Research Products

• [Journal Article] Anomalous oxygen consumption in porcine somatic cell nuclear transfer embryos (2010)
  • Author(s): Sugimura, S., Yokoo, M., Yamanaka, K., Kawahara, M., Moriyasu, S., Wakai, T., Nagai, T., Abe, H., Sato, E.
  • Journal Title: Cell.Reprogram (in press)
  • Peer Reviewed
• [Journal Article] Improving the quality of miniature pig somatic cell nuclear transfer blastocysts : aggregation of SCNT embryos at the four-cell stage (2010)
  • Author(s): Terashita, Y., Sugimura, S., Kudo, Y., Amano, R., Hiradate, Y., Sato, E.
  • Journal Title: Teprod.Domest.Anim. (in press)
  • Peer Reviewed
• [Journal Article] Effects of aminoguanidine on infertile varicocelized rats : A functional and morphological study (2010)
  • Author(s): Alizadeh, N., Abbasi, M., Abollhassani, F., Amidi, F., Mahmoudi, R., Hoshino, Y., Sato, E., Ragerdikashani, I.
  • Journal Title: DARU 18(1) Pages: 51-56
  • Peer Reviewed
• [Journal Article] Early metaphase II oocytes treated with dibutyryl cyclic adenosine monophosphate provide suitable recipient cytoplasm for the production of miniature pig somatic cell nuclear transfer embryos (2010)
  • Author(s): Sugimura, S, Yamanaka, K., Kawahara, M., Wakai, T., Yokoo, M., Sato, E.
  • Journal Title: Anim.Sci.J. 81 Pages: 48-57
  • NAID: 10027888500
  • Peer Reviewed
• [Journal Article] Lactate and adenosine triphosphate in the extender enhance the cryosurvival of rat epididymal sperm (2010)
  • Author(s): Yamashiro, H., Toyomizu, M., Kikusato, M., Toyama, N., Sugimura, S., Hoshino, Y., Abe, H., Moisyadi, S., Sato, E.
  • Journal Title: J.Am.Assoc.Lab.Anim.Sci. 49(2) Pages: 160-166
  • Peer Reviewed
• [Journal Article] Extracellular ATP and dibutyryl cAMP confer freezability on and enhanced the fertilizing ability of rat epididymal sperm (2010)
  • Author(s): Yamashiro H, Toyomizu M, Toyama N, Aono N, Sakurai M, Hiradate Y, Yokoo M, Moisyadi S, Sato E.
  • Journal Title: J.Am.Assoc.Lab.Anim.Sci. 49(2) Pages: 167-172
  • Peer Reviewed
• [Journal Article] Influence of the prostatic fluid from the first and third fractions of the ejaculates on the cryosurvival of the poodle dog sperm (2009)
  • Author(s): Yamashiro, H., Narita, K., Sugimura, S., Sugawara, A., Hoshino, Y., Sakurai, M., Yokoo, M., Konno, T., Yoshida, M., Sato, E.
  • Journal Title: Am.J.Anim.Vet.Sci. 4(1) Pages: 14-20
  • Peer Reviewed
• [Journal Article] Nitric oxide acts through different signaling pathway in maturation of cumuls cell-enclosed mouse oocytes (2009)
  • Author(s): Abbasi, M., Akbari, M., Amidi, F., Ragerdi, K. I., Mahmoudi, R., Sobahani, A., Takzare, N., Pasbakhsh, P., Barbarestani, M., Abolhassani, F., Sato, E.
  • Journal Title: DARU 17(1) Pages: 48-52
  • Peer Reviewed
• [Journal Article] Difference in sensitivity to culture condition between in vitro fertilized and somatic cell nuclear transfer embryos in pig (2009)
  • Author(s): Yamanaka, K., Sugimura, S., Wakai, T., Kawahara, M., Sato, E.
  • Journal Title: J.Reprod.Dev. 55(3) Pages: 299-304
  • NAID: 10024942485
• [Journal Article] Birth of somatic cell nuclear transfer (SCNT)-cloned miniature piglet following co-transfer parthenogenetic embryos (2009)
  • Author(s): Sugimura, S, Yamanaka, K., Wakai, T., Kawahara, M., Tanaka, H., Kobayashi, J., Kobayashi, E., Sato, E.
  • Journal Title: Tohoku J.Agr.Res. 59(3, 4) Pages: 51-61
  • NAID: 120001905973
  • Peer Reviewed
• [Journal Article] Interspecies somatic cell nuclear transfer with porcine oocytes as recipients : A novel bioassay system for assessing the competence of canine somatic cells to develop into embryos (2009)
  • Author(s): Sugimura, S, Narita, K., Yamashiro, H., Sugawara, A., Shoji, T., Terashita, Y., Nishimori, K., Konno, T., Yoshida, M., Sato, E.
  • Journal Title: Theriogenology 72 Pages: 549-559
  • Peer Reviewed
• [Journal Article] Tubulointerstitial nephritis antigen-like 1 is expressed in extraembryonic tissue and interacts with laminin-1 in the Reichert membrane at postimplantation (2009)
  • Author(s): Igarashi, T., Tajiri, Y., Sakurai, M., Sato, E., Li, D., Mukai, K., Suematsu, M., Fukui, E., Yoshizawa, M., Matsumoto, H.
  • Journal Title: Biol.Reprod. 81 Pages: 948-955
  • Peer Reviewed
• [Journal Article] Acetylation level of histone H3 in early embryonic stages affects subsequent development of miniature pig somatic cell nuclear transferembryos (2009)
  • Author(s): Yamanaka, K., Sugimura, S., Wakai, T., Kawahara, M., Sato, E.
  • Journal Title: J.Reprod.Dev. 55
  • NAID: 10026322712
  • Peer Reviewed
• [Journal Article] Oxidation of exogenous lactate by lactate dehydrogenase C in the midpiece of rat epididymal sperm is essential for motility and oxidative activity (2009)
  • Author(s): Yamashiro H, Toyomizu M, Kadowaki A, Takeda Z, Nakazato F, Toyama N, Kobayashi J, Sato E.
  • Journal Title: Am.J.Applied Sci. 6(10) Pages: 1854-1859
  • Peer Reviewed
• [Journal Article] Expression of hyaluronan synthase 1 and distribution of hyaluronan during follicular atresia in pig ovaries (2009)
  • Author(s): Miyake, Y., Sakurai, M., Tanaka, S., Tunjung, W. A. S., Yokoo, M., Matsumoto, H., Aso, H., Yamaguchi, T., Sato, E.
  • Journal Title: Biol.Reprod. 80 Pages: 249-257
  • Peer Reviewed
• [Journal Article] Establishment of rat embryonic stem-like cells from the morula using a combination of feeder layers (2009)
  • Author(s): Sano, C., Matsumoto, A., Sato, E., Fukui, E., Yoshizawa, M., Matsumoto, H.
  • Journal Title: Zygote 17 Pages: 229-237
  • Peer Reviewed
• [Journal Article] Effect of hyaluronan to inhibit caspase activation in porcine Granulosa cells (2009)
  • Author(s): Tunjung, W. A. S., Yokoo, M., Miyake, Y., Hoshino, Y., Sato, E.
  • Journal Title: Biochem.Biophys.Res.Communn. 382 Pages: 160-164
  • Peer Reviewed
• [Journal Article] Development and spindle formation in rat somatic cell nuclear transfer (SCNT) embryos in vitro using porcine recipient oocytes (2009)
  • Author(s): Sugawara, A., Sugimura, S. Hoshino, Y., Sato, E.
  • Journal Title: Zygote 17 Pages: 195-202
  • Peer Reviewed
• [Journal Article] Akt expression in mouse oocytes matured in vivo and in vitro (2009)
  • Author(s): Cecconi, S., Rossi, G., Santilli A., Palmerini, M. G., Stefano, L. D., Hoshino, Y., Sato, E., Micchiarelli, G.
  • Journal Title: RBM Online
  • Peer Reviewed
• [Journal Article] Inhibitory action of FSH in apoptosis of granulosa cells in pig COCG culture (2009)
  • Author(s): 佐藤英明
  • Journal Title: Biochem. Biophys. Res. Commuu 382 Pages: 160-164
  • Peer Reviewed
• [Journal Article] Induction of estrus in pubertal miniature gilts (2008)
  • Author(s): Wakai, T., Tanaka, H., Yamanaka, K., Sugimura, S., Sasada, H., Kawahara, M., Kobayashi, E., Sato, E.
  • Journal Title: Anim.Reprod.Sci. 103 Pages: 193-198
  • Peer Reviewed
• [Journal Article] Gene injections of vascular endothelial growth factor (VEGF) and growth differentiation factor-9 (GDF-9) stimulate ovarian follicular development in immature female rats (2008)
  • Author(s): Shimizu, T., Iijima, K., Ogawa, Y., Miyazaki, H., Sasada, H., Sato, E.
  • Journal Title: Fertil.Steril. 89(suppl.1) Pages: 1563-1570
  • Peer Reviewed
• [Journal Article] Effect of cytochalasins B nd D on the developmental competence of somatic cell nuclear transfer embryos in miniature pigs (2008)
  • Author(s): Sugimura, S., Kawahara, M., Wakai, T., Yamanaka, K., Sasada, H., Sato, E.
  • Journal Title: Zygote 16 Pages: 153-159
  • Peer Reviewed
• [Journal Article] Production of viable cloned miniature pig embryos using oocytes derived from domestic pig ovaries (2008)
  • Author(s): Wakai, T., Sugimura, S., Yamanaka, K., Kawahara, M., Sasada, H., Tanaka, H., Ando, A., Kobayashi, E., Sato, E.
  • Journal Title: Cloning Stem Cell. 10 Pages: 249-262
  • Peer Reviewed
• [Journal Article] In vitro induction of potential primordial germ cells from mouse embryonic stem cells by culture with undifferentiated gonadal cells (2008)
  • Author(s): Tomioka, I., Honma, Y., Sasada, H., Sato, E.
  • Journal Title: J.Mamm.Ova Res. 25 Pages: 37-43
  • NAID: 10021217689
  • Peer Reviewed
• [Journal Article] Noninvasive measurement of fecal progesterone concentration in toy poodles by time resolved fluoroimmunoassay (TR-FIA) (2008)
  • Author(s): Sugimura, S., Narita, K., Yamashiro, H., Sugawara, A., Nishimori, K., Konno, T., Yoshida, M., Sato, E.
  • Journal Title: Am.J.Anim.Vet.Sci. 3(1) Pages: 43-46
  • Peer Reviewed
• [Journal Article] Thyroxine treatment stimulated ovarian follicular angiogenesis in immature hypothyroid rat (2008)
  • Author(s): Jiang, J. Y., Miyabayashi, K., Nottola, S. A., Umezu, M., Cecconi, S., Sato, E., Macchiarelli, G.
  • Journal Title: Histol Histopathol. 23 Pages: 1387-1398
  • Peer Reviewed
• [Journal Article] Influence of hyaluronan accumulation during cumulus expansion on in vitro porcine oocyte maturation (2008)
  • Author(s): Yokoo, M., Kimura, N., Abe, H., Sato, E.
  • Journal Title: Zygote 16 Pages: 309-314
  • Peer Reviewed
• [Journal Article] Protein kinase B (PKB/Akt) is required for the completion of meiosis in mouse oocytes (2008)
  • Author(s): Hoshino, Y., Sato, E.
  • Journal Title: Dev.Biol. 314 Pages: 215-223
  • Peer Reviewed
• [Journal Article] Production of a large number of ovulation oocytes by exogenous gene njection in mammalian ovary (2008)
  • Author(s): 佐藤英明
  • Journal Title: Fert. Steril. 89 Pages: 1563-1570
  • Peer Reviewed
• [Journal Article] Production of viable cloned miniature pig embryos using oocvtes derived from domestic pig ovaries (2008)
  • Author(s): 佐藤英明
  • Journal Title: Cloning Stem Cell 10 Pages: 249-262
  • Peer Reviewed
• [Journal Article] Effect of activation treatments on actin filament distribution and in vitro development of miniature pig somatic cell nuclear transfer embryos (2007)
  • Author(s): Yamanaka, K., Sugimura, S., Waskai, T., Shoji, T., Kobayashi, J., Sasada, H., Sato, E.
  • Journal Title: J.Reprod.Dev. 53 Pages: 791-800
  • NAID: 10020003798
  • Peer Reviewed
• [Journal Article] Trehalose enhanced the freezability of poodle dog sperm collected by an artificial vagina (A.V.) (2007)
  • Author(s): Yamashiro, H., Narita, K., Sugimura, S., Han, Y. J., Sugawara, A., Morohaku, K., Nakazato, F., Konno, T., Yoshida, M., Sato, E.
  • Journal Title: Anim.Reprod.Sci. 102 Pages: 165-171
  • Peer Reviewed
• [Journal Article] Gene expression profiles of novel caprine placental prolactin-related proteins similar to bovine placental prolactin-related proteins (2007)
  • Author(s): Ushizawa, K., Takahashi, T., Hosoe, M., Kizaki, K., Abe, Y., Sasada, H., Sato, E., Hashizume, K.
  • Journal Title: BMC Dev.Biol. 7:16 Pages: 1-13
  • NAID: 120002337684
  • Peer Reviewed
• [Journal Article] Freezability of rat epididymal sperm induced by raffinose in modified Krebs-Ringer Bicarbonate (mKRB) based extender solution (2007)
  • Author(s): Yamashiro, H., Han, Y. J., Sugawara, A., Tomioka, I., Hoshino, Y., Sato, E.
  • Journal Title: Cryobiology 55 Pages: 285-294
  • Peer Reviewed
• [Journal Article] Spindle formation and microtubule organization during first division in reconstructed rat embryos produced by somatic cell nuclear transfer (2007)
  • Author(s): Tomioka, I., Mizutani, E., Yoshida, T, Sugawara, A., Inai, K., Sasada, H., Sato, E.
  • Journal Title: J.Reprod.Dev. 53(4) Pages: 835-842
  • NAID: 10020003952
  • Peer Reviewed
• [Journal Article] Cellular and molecular events during oocyte maturation in mammals : Molecules of cumulus-oocyte complex matrix and signaling pathways regulating meiotic progression. (2007)
  • Author(s): Eimei Sato
  • Journal Title: Reproduction. (in press)
• [Journal Article] Role of the hyaluronan receptor CD44 during Porcine oocyte Maturation (2007)
  • Author(s): Eimei Sato
  • Journal Title: J.Reprod.Dev. (in press)
  • NAID: 10027327814
• [Journal Article] Morphodynamics of ovarian follicles during oogenesis in mice. (2006)
  • Author(s): Eimei Sato
  • Journal Title: Microsc.Res.Techniq. 69(6) Pages: 427-435
• [Journal Article] Morphological patterns of angiogenesis in ovarian follicle capillary networks. A scanning electron microscopy study of corrosio cast. (2006)
  • Author(s): Eimei Sato
  • Journal Title: Microsc.Res.Techniq. 69(6) Pages: 459-468
• [Journal Article] A new cell death inhibitor, Bax-inhibiting peptide(BIP) derived from Ku70 (2006)
  • Author(s): Eimei Sato
  • Journal Title: Tohoku.J.Agr.Res. 56 Pages: 37-44
• [Journal Article] Involvement of vascular endothelial growth factor in the formation of thecal layer and vasculature during follicular development in ovaries of neonatal female rats. (2006)
  • Author(s): Eimei Sato
  • Journal Title: Anim.Sci.J. 77 Pages: 574-581
  • NAID: 10021223633
• [Journal Article] Expression and glycosylation with polylactosamine of CD44 antigen on macrophages during follicular atresia in pig ovaries. (2006)
  • Author(s): 佐藤英明
  • Journal Title: Biol.Reprod. (in press)
• [Journal Article] PKA is implocated in the phosphorylation of Cx43 induced by stimulation with FSH in rat granulosa cells (2006)
  • Author(s): 竹塚達夫
  • Journal Title: J.Reprod.Dev (in press)
• [Journal Article] Changes of mRNA expression of vascular endothelial growth factor (VEGF), angiopoietins and their specific receptors during the periovulatory phase in eCG/hCG-primed immature female rats. (2005)
  • Author(s): 佐藤英明
  • Journal Title: J.Exp.Zool 303A Pages: 590-597
• [Journal Article] Production of live offsprings from mouse germinal vesicle-stage oocytes vitrified by a modified step-wise method, SWEID. (2005)
  • Author(s): 佐藤英明
  • Journal Title: Fertil.Steril 84(suupl.2) Pages: 1078-1082
• [Journal Article] Changes in Expression and Localization of Connexin 43 mRNA and Protein in Porcine Ovary Granulosa Cells during Follicular Atresia. (2005)
  • Author(s): 眞鍋昇
  • Journal Title: J.Reprod.Dev 51(5) Pages: 627-637
  • NAID: 10027326025
• [Journal Article] Feasibility of a nylon mesh holder for vitrification of bovine germinal vesicle oocytes in subsequent production of viable blastocysts (2005)
  • Author(s): 佐藤英明
  • Journal Title: Biol.Reprod. (in press)
• [Journal Article] Molecular cloning and functional analysis of a novel gene KDN encoding Cx43 partner protein (2005)
  • Author(s): 竹家達夫
  • Journal Title: J.Biol.Chem (in press)
• [Journal Article] Molecular cloning of porcine growth differentiation factor 9(GDF-9) cDNA and its role in early folliculogenesis : direct ovarian injection of GDF-9 gene fragments promotes early folliculogenesis. (2004)
  • Author(s): 佐藤英明
  • Journal Title: Reproduction 128・5 Pages: 537-543
• [Journal Article] Differential expression of bone morphogenetic protein 4,5,6(BMP-4,-5,-6) and growth differentiation factor-9(GDF-9) during ovarian development in neonatal pigs (2004)
  • Author(s): 佐藤英明
  • Journal Title: Domesric Animal Endocrinology 27・3 Pages: 397-405
• [Journal Article] The porcine (Sus scrofa) cellular Flice-like inhibitory protein(cFLIP) : molecular cloning and comparison with the human and murine cFLIP. (2004)
  • Author(s): 眞鍋 昇
  • Journal Title: J.Reprod.Dev. 50・(6) Pages: 549-555
  • NAID: 10027323381
• [Journal Article] The differential coupling of oxytocin receptors to uterine contractions in murine estrous cycle (2004)
  • Author(s): 西森克彦
  • Journal Title: Biochem.Biophys.Res.Commun. 321・5 Pages: 695-699
• [Journal Article] LGR4/Gpr48 is essential for renal development/function in mice
  • Author(s): 西森克彦
  • Journal Title: J.Endcrinol submitted
• [Presentation] Recent research trends inanimal reproduction and biotechnology in Japan (2010)
  • Author(s): Sato, E.
  • Place of Presentation: University Malaysia Sabah
  • Year and Date: 2010-03-16
• [Presentation] Outline and activities of Tohoku University (2010)
  • Author(s): Sato, E.
  • Organizer: Laboratory of Animal Reproduction. School of Sustainable Agriculture
  • Place of Presentation: University Malaysia Sabah
  • Year and Date: 2010-03-15
• [Presentation] Expression of hyaluronan synthase 1 and distribution of hyaluronan during ovarian follicular atresia in pig ovaries (2009)
  • Author(s): Miyake, Y., Sakurai, M., Tunjung, W. A. S., Sato, E.
  • Organizer: The 3rd Congress of Asian Association of Veterinary Anatomists & The 29th Annual Meeting of Korean Association of Veterinary Anatomists
  • Place of Presentation: Chungbuk National University
  • Year and Date: 2009-11-04
• [Presentation] Local regulators affecting ovarian function-Role of capillary blood vessels and macrophages- (2009)
  • Author(s): Sato, E.
  • Organizer: The 7th Asian Symposium on Animal Biotechnology-Joint with 2009 Annual Conference of Korean Scociety of Animal Reproduction
  • Place of Presentation: Chungbuk National University, Cheonju, Korea
  • Year and Date: 2009-06-19
• [Presentation] Outline and activities of Tohoku University (2009)
  • Author(s): Sato, E.
  • Organizer: Laboratory of Animal Reproduction. The Open Seminar of CVMBS
  • Place of Presentation: Cavite State University, Philippine
  • Year and Date: 2009-05-09
• [Presentation] Inhibition of mTOR induces cumulus expansion and meiotic maturation in mouse oocytes without gonadotropin stimulation (2009)
  • Author(s): Hoshino, Y., Sato, Y., Sakai, C., Sato, E.
  • Organizer: SSR 42nd annual meeting, David L. Lawrence Convention Center
  • Place of Presentation: Pittsburgh, Pennsylvania
• [Presentation] Addition of a phosphatase inhibitor prompts growth initiation of isolated primordial follicles oocytes in culture (2009)
  • Author(s): Morohaaku, K., Hoshino, Y., Sato, E.
  • Organizer: SSR 42nd annual meeting, David L. Lawrence Convention Center
  • Place of Presentation: Pittsburgh, Pennsylvania
• [Presentation] Utilization of exogenous lactate by lactate dehydrogenase^C in the midpiece of rat epididymal sperm is essential for motility and oxidative activity (2009)
  • Author(s): Yamashiro, H., Toyomizu, M., Aono, A., Sakurai, M., Nakazato, F., Kadowaki, A., Yokoo, M., Hoshino, Y., Sato, E.
  • Organizer: 10th World Conference on Animal Production (International Convention Center, Cape Town, South Africa, November 22-28, 2008, Book of Abstract for the 10th World Conference on Animal Production)
  • Place of Presentation: University, Korea
• [Presentation] Factors affecting the production of somatic muclear cloned miniature pigs (2009)
  • Author(s): Sato, E.
  • Organizer: The 6rh Annual Conference of the Asian Reproductive Biotechnology Society
  • Place of Presentation: Siem Reap, Cambodia
• [Presentation] Role of capillary blood vessels and macrophages in follicular development (2009)
  • Author(s): Sato, E.
  • Organizer: 36th International Congress of Physiological Sciences (IUPS2009)
  • Place of Presentation: Kyoto, Japan
• [Presentation] Recent progress of animal reproduction in Japan (2008)
  • Author(s): Sato, E.
  • Organizer: The 4th JSAR-KSAR Joint Symposium
  • Place of Presentation: Konkuk University, Seoul, Korea
  • Year and Date: 2008-06-20
• [Presentation] Evaluation of the fertilizing ability of bovine sperm by acridine orange staining techniques (2008)
  • Author(s): Kohsaka, T., Yogo, K., Kaneko, N., Kuwahara, Y., Watanabe, S., Sasada, H., Sato, E.
  • Organizer: 1st Congress on Reprodictibe Biology
  • Place of Presentation: Kona, Hawaii, USA
  • Year and Date: 2008-05-24
• [Presentation] The development of ICS embryo after remov acrosome and nuclear deconsation in bovine (2008)
  • Author(s): Sato, E., Takahashi, T.
  • Organizer: The 13th Animal Science Congress of AAAP (International Conference Center)
  • Place of Presentation: Hanoi, Vietnam
• [Presentation] Mitochondrial function in porcine somatic cell nuclear transferred embryos (2008)
  • Author(s): Sugimura, S., Yokoo, M., Yamanaka, K., Abe, H., Sato, E.
  • Organizer: The 13th Animal Science Congress of AAAP (International Conference Center)
  • Place of Presentation: Hanoi, Vietnam
• [Presentation] VEGF and GDF-9 gene fragments promote follicular development in the ovaries of pigs (2008)
  • Author(s): Sato, E., Oshino, Y., Sakurai, Y.
  • Organizer: 15th Intional Congress on Technology in Animal Reproduction
  • Place of Presentation: Bangladesh Agricultural University, Mymensingh, Bangkadesh
• [Presentation] Effect of epigenetic histone modification of donar somatic cells on developmental competence of cloned mouse embryos and establishment efficiency of ntES cell line (2008)
  • Author(s): Baba, K., Sugimura, S., Sato, E.
  • Organizer: The 1st Chungbuk National University and Tohoku University Joint Seminar on Animal Reproduction
  • Place of Presentation: Chungbuk National University, Cheonju, Korea
• [Presentation] Expression and dynamics of adrenocortical zonation factor 1 (AZ-1) and its high affinity extracellular components (fibronectin and collagen type IV) during mouse early embryogenesis (2008)
  • Author(s): Sakurai, M., Sato, Y., Hoshino, Y., Sato, E.
  • Organizer: The 1st Chungbuk National University and Tohoku University Joint Seminar on Animal Reproduction
  • Place of Presentation: Chungbuk National University, Cheonju, Korea
• [Presentation] Akt is functional in the completion of meiosis in mouse oocytes (2008)
  • Author(s): Hoshino, Y., Sato, E.
  • Organizer: The Society for Reproduction and Fertility Conference and exhibition 2008
  • Place of Presentation: University of Edinburgh, UK
• [Presentation] Exogenous lactate and adenosine triphosphate in extender solution regulates the oxidative activity and enhanced the freezability of rat epididymal sperm (2008)
  • Author(s): Yamashiro, H., Toyomizu, M., Han, Y. J., Yokoo, M., Abe, h., Hoshino, Y., Sato, E.
  • Organizer: The Society for Reproduction and Fertility Conference and exhibition 2008
  • Place of Presentation: University of Edinburgh, UK
• [Presentation] Evaluation of mitochondrial function in porcine somatic cell nuclear transfer (SCNT) embryos (2008)
  • Author(s): Sugimura, S., Yokoo, M., Yamanaka, K., Abe, H., Sato, E.
  • Organizer: The 1st Chungbuk National University and Tohoku University Joint Seminar on Animal Reproduction
  • Place of Presentation: Chungbuk National University, Cheonju, Korea
• [Presentation] Research topics, Laboratory of Animal Reproduction, Tohoku University (2008)
  • Author(s): Sato, E.
  • Organizer: The 1st Chungbuk National University and Tohoku University Joint Seminar on Animal Reproduction
  • Place of Presentation: Chungbuk National University, Cheonju, Korea
• [Presentation] Angiogenesis and vascular regulation of reproductive function, Comments (2008)
  • Author(s): Sato, E.
  • Organizer: 1st World Congress on Reproductive Biology
  • Place of Presentation: Kona, Hawaii, USA
• [Presentation] Recent research trends in animal reproduction and biotechnology in Japan (2007)
  • Author(s): Sato, E.
  • Organizer: The 3rd Korea-Japan Jpoint symposium of animal reproductive Proceedings, biology and biotechnology
  • Place of Presentation: University of Tokyo
  • Year and Date: 2007-10-18
• [Presentation] Development of capillary blood vessels during follicular develo bment and its artificial control by VEGF gene constructs (2007)
  • Author(s): 佐藤英明
  • Organizer: The 2nd Congress of the Asian Association of Veterinary Anatomists
  • Place of Presentation: バンコク/タイ
  • Year and Date: 2007-09-13
• [Presentation] Evaluation of mitochondrial function in somatic cell nuclear transferred embryos (2007)
  • Author(s): Sugimura, S., Yokoo, M., Yamanaka, K., Hoshino, Y., Abe, H., Sato, E.
  • Organizer: 4th Canada-Japan Bilateral Workshop on Human Reproduction & Reproductive Biology
  • Place of Presentation: Hirosaki City Hotel, Hirosaki
  • Year and Date: 2007-08-01
• [Presentation] Manipulation of ovarian follicle development by injecting vascular endothelial growth factor (VEGF) gene (2007)
  • Author(s): Sato, E.
  • Organizer: 4th Canada-Japan Bilateral Workshop on Human Reproduction & Reproductive Biology
  • Place of Presentation: Hirosaki City Hotel, Hirosaki
  • Year and Date: 2007-08-01
• [Presentation] Inhibitory action of FSH in apoptosis of swine granulosa cells in COCG culture involves hyaluronan synthesis in an intrinsic pathway (2007)
  • Author(s): Tunjung, W. A. S., Yokoko, M., Miyake, Y., Sato, E.
  • Organizer: 40th Annual Meeting of Society for the Study of Reproduction
  • Place of Presentation: Marriott San Antonio Rivercenter, San Antonio, Texas
  • Year and Date: 2007-07-02
• [Presentation] Cultivation period for maturation of recipient oocytes and the developmental ability of cloned porcine embryos (2007)
  • Author(s): Wakai, T., Sugimura, S., Yamanaka, K., Sato, E.
  • Organizer: 40th Annual Meeting of Society for the Study of Reproduction
  • Place of Presentation: Marriott San Antonio Rivercenter, San Antonio, Texas
  • Year and Date: 2007-06-30
• [Presentation] Recent research trends inanimal reproduction and biotechnology in Japan (2007)
  • Author(s): Sato, E.
  • Organizer: 6th CJK symposium
  • Place of Presentation: Choongang University, Korea
  • Year and Date: 2007-06-28
• [Presentation] Expression of phagocytic receptor CD44 and sus scrofa hyaluronan synthase 1 on macrophages during follicular atresia in pig ovaries (2007)
  • Author(s): Miyake, Y., Tunjung, W. A., Sato, E.
  • Organizer: The International Ovarian Conference 2007 Hakone
  • Place of Presentation: Hakone, Japan
• [Presentation] Expression of WNT/STAT3 signaking and their role on the rat embryogenesis (2007)
  • Author(s): Han, Y. J., Hoshino, Y., Yamashiro, H., Kang, M. S., Sato, E.
  • Organizer: The 7th International Symposium on Developmental Biotechnology
  • Place of Presentation: Seoul, Korea
• [Presentation] Evaluation of cuture medium for in vitro developmental competence in porcine in vitro matured oocytes (2007)
  • Author(s): Hoshino, Y., Abe, H., Wakai, T., Yamanaka, K., Sugimura, S., Sato, E.
  • Organizer: 14th World Congress on In Vitro Fertilization and 3rd World Congress on In Vitro Maturation
  • Place of Presentation: Montreal, Canada
• [Presentation] Evaluation of mitochondrial function in single cloned miniature pig embryos by measuring oxygen consumption (2007)
  • Author(s): Sugimura, S., Yokoo, M., Yamanaka, K.-I, Wakai, T., Abe, H., Shoji, T., Sasada, H., Kobayashi, J., Abe, H., Sato, E.
  • Organizer: The Annual Conference of the International Embryo Transfer Society
  • Place of Presentation: Kyoto, Japan
• [Presentation] Motility and fertility of bull spermatozoa frozen in egg yolk extender supplemented with lactoferrin (2007)
  • Author(s): Kobayashi, J., Suda, Y., Takada, N., Komi, K., Kuroishi,., Kohsaka, T., Sasada,., Sato, E.
  • Organizer: The Annual Conference of the International Embryo Transfer Society
  • Place of Presentation: Kyoto, Japan
• [Presentation] Development of capillary blood vessels during follicular development and its artificial control by VEGF gene constructs (2007)
  • Author(s): Sato, E., Hoshino, Y.
  • Organizer: The 2nd Congress of the Asian Association of Veterinary Anatomists
  • Place of Presentation: Bangkok, Thailand
• [Presentation] Factors regarding success for production of somatic nuclear cloned miniature pigs (2007)
  • Author(s): Sato, E., Sugimura, S.
  • Organizer: 14th International Conference on Biotechnology in Animal Reproduction
  • Place of Presentation: Cairo, Egypt
• [Presentation] Manipulation of ovarian follicle development by injecting vascular endothelial growth factor (VEGF) gene (2007)
  • Author(s): Sato, E.
  • Organizer: VII Italian Congress of Veterinary Physiology
  • Place of Presentation: Silvi Marina (Teramo)
• [Book] 卵子の成熟-卵子成熟のメカニズム (基礎)、カラーアトラス不妊治療のための卵子学 (鈴木秋悦編) (2010)
  • Author(s): 佐藤英明・星野由美・横尾正樹
  • Publisher: 医歯薬出版
• [Book] 畜産学の視点-畜産学の立脚点を考える (2009)
  • Author(s): 佐藤英明
  • Publisher: 社団法人畜産技術協会
• [Book] 全国体外受精実施施設完全ガイドブック2009 (不妊治療情報センター編) (2009)
  • Author(s): 佐藤英明
  • Publisher: 日本受精着床学会
• [Book] 「相利共生」の要としての畜産創造に向けて、年版 農業技術 畜産2008 (2008)
  • Author(s): 佐藤英明
  • Publisher: 農文協
• [Book] ES細胞と生殖医療、不妊症-臨床と研究の最前線 (吉村泰典編) (2008)
  • Author(s): 佐藤英明・星野由美
  • Publisher: 医歯薬出版
• [Book] 受精、獣医発生学 (谷口和之・木曾康郎・佐藤英明、監修) (2008)
  • Author(s): 佐藤英明
  • Publisher: 学窓社
• [Book] Cellular and molecular events during oocyte maturation in mammals: Molecules of cumulus-oocyte complex matrix and signaling pathways regulating meiotic progression (2008)
  • Author(s): 佐藤英明
  • Total Pages: 548
  • Publisher: Gamete Biology, Emerging Frontiers in Fertility and Contraceptive Development
• [Book] Cellular and molecular events during oocyte maturation in mammals : Molecules of cumulus-oocyte complex matrix and signaling pathways regulating meiotic progression. In Gamete Biology, Emerging Frontiers in Fertility and Contraceptive Development (Edited by Gupta, S. K., Koyama, K., Murray, J. F.) (2007)
  • Author(s): Kimura, N., Hoshino, Y., Totsukawa, K., Sato, E.
  • Publisher: Nottingham University Press
• [Book] Recent research trends in animal reproduction and biotechnology in Japan In Current Status and Perspectives in animal reproduction and safety assessment of cloning animals (Edited by Manabe, N.) (2007)
  • Author(s): Sato, E.
• [Book] 図説ARTマニュアル 改訂第2版 (2006)
  • Author(s): 佐藤英明
  • Total Pages: 538
  • Publisher: 永井書店
• [Book] 生殖補助医療(ART)胚培養の理論と実際(日本哺乳動物卵子学会編) (2005)
  • Author(s): 佐藤英明
  • Total Pages: 274
  • Publisher: 近代出版
• [Book] 哺乳類の卵細胞 (2004)
  • Author(s): 佐藤英明
  • Total Pages: 114
  • Publisher: 朝倉書店
• [Remarks]
  • URL: http://www.agri.tohoku.ac.jp/seisyoku/index-j.html
• [Remarks]
  • URL: http://www.agri.tohoku.ac.jp/seisyoku/index-j.html