| Budget Amount *help |
¥48,230,000 (Direct Cost: ¥37,100,000、Indirect Cost: ¥11,130,000)
Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2006: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2005: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
Fiscal Year 2004: ¥21,190,000 (Direct Cost: ¥16,300,000、Indirect Cost: ¥4,890,000)
|
|---|
| Research Abstract |
For clinical operativity of bone regeneration, adequate committed osteoblasts are required. Bone marrow mesenchymal stromal cells (MSCs) could provide a source for bone regeneration. In addition, it is well known that Mesenchymal cell condensation plays an important role in the early stages of membranous osteogenesis and dynamic flow can positively influence and enhance osteodifferentiation of MSCs. Therefore, to imitating bone development, the rotation culture method was adopted in our experiments as compared with static culture. Osteogenic differentiation was evaluated by histological, immunochemical and RT-PCR analyses. We showed that the cell condensation and three-dimensional dynamic environment in rotation culture may enhance osteoblastic phenotype development and mineralized matrix synthesis in vitro. The extracellular matrix of cell aggregates was endogenously synthesized entirely naturally by the cells themselves. The cell aggregate by rotation culture showed mineral deposition and osteocalcin expression at 7 days, whereas static culture was detected at 14 days. Rotation culture is a much more closely resemble the cell environment in vivo and suitable culture environment than static culture in well plates.
|
