| Project/Area Number |
16206076
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Properties in chemical engineering process/Transfer operation/Unit operation
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| Research Institution | Okayama University |
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Principal Investigator |
NAKANISHI Kazuhiro Okayama University, Graduate School of Natural Science and Technology, Professor (90026584)
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| Co-Investigator(Kenkyū-buntansha) |
IMAMURA Koreyoshi Okayama University, Graduate School of Natural Science and Technology, Associate Professor (70294436)
IMANAKA Hiroyuki Okayama University, Graduate School of Natural Science and Tochnology, Assistant Professor (10379711)
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| Project Period (FY) |
2004 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2007: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2006: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2005: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2004: ¥26,780,000 (Direct Cost: ¥20,600,000、Indirect Cost: ¥6,180,000)
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| Keywords | adsorption / adsorption equilibrium / protein / Peptide / metal oxide surface / plastic surface / cleaning / hydrogen peroxide-electrolysis cleaning / 表面プラズモン共鳴 / 脂肪酸・油脂の付着 / 親和性ペプチド / 付着機構 / 吸着等温線 / β-ラクトグロブリン / 脱離速度 / ポリスチレン / 付着 / 固体表面 / アフィニティペプチド / 疎水性・親水性 / GST / ELISA / カルボキシル基 / アミノ基 / ステンレス鋼板 / 希少金属酸化物 / 高感度反射法 / 吸着等温式 / 付着の配向 |
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| Research Abstract |
In various manufacturing processes, cleaning of the equipment and piping systems used for manufacturing of various products is requisite for maintaining sanitary conditions and/or functions of the equipments. In particular, in the food- and bio-products manufacturing processes, a large amount of energy and detergents are usually consumed in addition to time, since soils or residues of the products strongly interact with the equipment surface. In this study, we studied adsorption mechanisms of various proteins/peptides and low-molecular weight substances as a model soil component on the surfaces of various metal oxides, plastics, and glasses. We clarified the molecular mechanisms for reversible/irreversible adsorptions, states and orientation of the molecules adsorbed, the effects of the pI values of the metal oxide surfaces/solution pHs on the adsorption characteristics, and conformational change of the adsorbed proteins, on the basis of adsorption/desorption experiments, IR spectrum analyses, and molecular calculations. Furthermore, we investigated the effects of various factors such as the kinds of the co-existing salts and their concentrations on the removal rates of the metal oxide surface fouled with proteins, using H2O2-electrolysis cleaning method that was developed by the author's group. Furthermore, we developed a method to immobilize proteins with controlled orientation and structure for various uses in chip-based biotechnology, which was derived in the course of investigation on the adsorption mechanism of proteins.
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