| Project/Area Number |
16209043
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
KUROSAWA Hiromi Tokyo Women's Medical University, School of Medicine, Professor, 医学部, 教授 (50075511)
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| Co-Investigator(Kenkyū-buntansha) |
SHIN'OKA Toshiharu Tokyo Women's Medical University, School of Medicine, Assistant Professor, 医学部, 助教授 (20192122)
MATSUOKA Rumiko Tokyo Women's Medical University, School of Medicine, Associate Professor, 医学部, 講師 (50120051)
MATSUMURA Goki Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助手 (20297469)
IMAMURA Shinichiro Tokyo Women's Medical University, School of Medicine, Assistant, 医学部, 助手 (00176497)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥41,210,000 (Direct Cost: ¥31,700,000、Indirect Cost: ¥9,510,000)
Fiscal Year 2005: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Fiscal Year 2004: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
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| Keywords | Regenerative Medicine / Tissue Engineering / Bone Marrow / Biodegradable Scaffold / Cardiac Cell / Gene Transfection |
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| Research Abstract |
We have previously reported the successful creation of tissue-engineered vascular structure and the 47 clinical implantation of these autologous tissue in the pulmonary circulation. Recently, several institutes started gene therapy and cell therapy in the clinical setting for the treatment of ischemic myocardial failure. In this study, we tried to establish the method to differentiate BMCs to myocardial phenotype in vitro, then implant to in vivo model. To obtain the beating cells in vitro from the GATA-4 transfected stormal cells in vitro, two differenct protocol was designed.
In protocol A, the effects of liquid factors from neonatoal myocytes was examined in a co-culture dish.
In protocol B, cell-to-cell interaction was examined using mixed culture system. The cell-sheet system will be applied to create adult cardiac cell sheet. Using a rat model, Dr Nadal-Ginard protocol (Mt Sainai, NY, USA) was applied and cultured stem cell will be grown in culture. After obtaining the sufficient number of cardiac stem cells, they will be implanted in a ischemic heart model of syngenic rat. All experiments are still underway.
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