| Project/Area Number |
16209063
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Periodontal dentistry
|
|---|
| Research Institution | Nihon University |
|---|
Principal Investigator |
ABIKO Yoshimitsu Nihon University, School of Dentistry at Matsudo, Professor (70050086)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KURIHARA Hidemi Hiroshima University, Graduate School of Biomedical Sciences, Professor (40161765)
MURAKAMI Shinya Osaka University, Graduate School of Dentistry, Professor (70239490)
NAKAYAMA Koji Nagasaki University, Graduate School of Biomedical Sciences, Professor (80150473)
AMANO Atsuo Osaka University, Graduate School of Dentistry, Professor (50193024)
TAKASHIBA Shogo Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Professor (50226768)
|
|---|
| Project Period (FY) |
2004 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥49,530,000 (Direct Cost: ¥38,100,000、Indirect Cost: ¥11,430,000)
Fiscal Year 2007: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2006: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2005: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2004: ¥21,190,000 (Direct Cost: ¥16,300,000、Indirect Cost: ¥4,890,000)
|
|---|
| Keywords | Periodontal Research / Development of treatment / Genomics / Proteomics / Transcriptomics / GeneChip / Target molecules / プロテオミクス / MALDI TOF-MS / 歯周病病原菌 / 歯周組織細胞 / ゲノム / トランスクリプトーム / プロテオーム / バイオインフォーティクス / データベース / シグナル伝達系 |
|---|
| Research Abstract |
Experimental research utilizing functional genomics technologies with DNA/protein database will serve to identification of genes and their expressions on the transcriptional level exemplify their utility for the understanding the life science. Several bacterial genome projects associated with oral infectious diseases are in progress. Human genome has been sequenced and assembled, making the accessible for genetic studies in near future. Recently, technological advances, such as subtractive gene cloning and DNA microarray, have been applied to discover of novel genes involved in complex metabolism. For example, since little is known regarding the molecules expressed by gingival epithelial cells that are involved in inflammation following the interaction with periodontal pathogens, to find transcriptional profiling of genes in human gingival epitherial cells in response to LPS, we examined many altered gene expressions using over 8,000 cDNA microarray (Incyte, GEM). To find transcriptional profiles of genes in submandibular gland by ageing, we examined mRNA levels of over 6,500 genes by nucleotide micreoarray (Affimetrix, GeneChip). Our laboratory involved in dental science projects using these technologies and genome database. By this Grant support, we plan to make the new database for oral diseases, which will be used easily by many researchers. The genome project and molecular biological approaches using the database will open the gate develop the dental science.
|
