• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies for diversity of modulation system on Pain and Capsaicin sensitivities

Research Project

Project/Area Number 16300138
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionNational Institute of Genetics

Principal Investigator

KOIDE Tsuyoshi  National Institute of Genetics, Associate Professor, 系統生物研究センター, 助教授 (20221955)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2005: ¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 2004: ¥7,800,000 (Direct Cost: ¥7,800,000)
KeywordsPain / Capsaicin / TRP-V1 / Mouse / Congenic strains / Thermo sensitivity
Research Abstract

In animal, heat stimulation is transmitted to central nervous system (CNS) via sensory neuron, and is recognized as heat or pain. Various levels of heat sensations activate the multi functional pain receptors, TRPV channels that expressed in sensory ganglia. Many behavioral tests had been established to measure the pain sensitivity in rodents by investigating behavioral response from the aversive pain stimuli. Hot plate test displays the ability of pain recognition system or analgesic system based on upper CNS. The tail flick test displays the pain sensitivity based on nociceptive reflex. In the present study, we conducted QTL analyses to characterize genetic loci involved in the different sensitivity to heat evoked pain between two mouse strains, C57BL/6 and KJR. In the study, we applied hot plate test and tail flick test to assess the two types of pain sensitivity of BKF2 progeny generated form F1 progeny of C57BL/6 and KJR. In the analyses, we detected three significant loci for hot … More plate test, Hpq1, Hpq2 and Hpq3 and two significant loci for tail flick test, Tfq1 and Tfq2. The pain associated quantitative traits loci, Hpq1, Hpq2, Hpq3, Tfq1, and Tfq2 were located on the chromosomes, 2,10,18,8 and 10, respectively. Hpq2 and Tfq1 were overlapped on the same region of chromosome 10. These data suggested that genetic mechanism for strain difference of pain sensitivities between C57BL/6 and KJR that mediated by upper CNS and reflection system are mostly different but partly shared.
To elucidate phenotype of thermo-sensation, we conducted recording of femoral nerve activity in vivo and intracellular free calcium (iCa) response to heat in vitro in mice of KJR. KJR is the mice strain known to be resistant to hot-plate test. Increment in neuronal activity from 25 C to 40 C was not different in KJR compared to the control. Whereas that from 25 C to 50 C was was different in KJR, when the degree of the increment was compared with C57BL/6 by two-way ANOVA. DRG neurons were isolated from these mice and iCa was measured by ratio methods with fluo-3 and fura-red. Surprisingly, all of the phenotypes including capsaicin resistance in KJR are not reproduced in isolated DRG neurons : We observed rise in iCa in some DRG neurons of KJR to heat and capsaicin. These results may suggest that thermo-sensation is a complicated sensory process involving contribution of proteins other than TRP channels and that the experiments in vitro and in vivo are needed to substantiate the phenotype. Less

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (16 results)

All 2006 2005 2004 Other

All Journal Article (13 results) Book (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Multivariate analysis of temporal descriptions of open-field behavior in wild derived mouse strains2006

    • Author(s)
      Takahashi et al.
    • Journal Title

      Behavior Genetics 10

      Pages: 1-12

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Multivariate analysis of temporal descriptions of open-field behavior in wild derived mouse strains.2006

    • Author(s)
      Takahashi, A., Kato, K., Makino, J., Shiroishi, T., Koide, T.
    • Journal Title

      Behavior Genetics 10

      Pages: 1-12

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Multivariate analysis of temporal descriptions of open-field behavior in wild derived mouse strains.2006

    • Author(s)
      Takahashi et al.
    • Journal Title

      Behavior Genetics (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Monoallelic yet combinatorial expression of variable exons of the protocadherin-a gene cluster in single neurons2005

    • Author(s)
      Esumi et al.
    • Journal Title

      Nature Genetics 37

      Pages: 171-176

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Length variation of CAG/CAA triplet repeats in 50 genes among 16 inbred mouse strains2005

    • Author(s)
      Ogasawara et al.
    • Journal Title

      Gene 349

      Pages: 107-119

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Molecular evolution of Cadherin-related neuronal receptor/protoca dherin α(CNR/Pcdhα) gene cluster in Mus musculus subspecies2005

    • Author(s)
      Taguchi et al.
    • Journal Title

      Mol. Biol. Evol. 22

      Pages: 1433-1443

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Length variation of CAG/CAA triplet repeats in 50 genes among 16 inbred mouse strains.2005

    • Author(s)
      Ogasawara, M., Imanishi, T., Moriwaki, K., Gaudieri, S., Tsuda, H., Hashimoto, H., Shiroishi, T., Gojobori, T., Koide, T.
    • Journal Title

      Gene 349

      Pages: 107-119

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Monoallelic yet combinatorial expression of variable exons of the protocadherin-a gene cluster in single neurons.2005

    • Author(s)
      Esumi, S., Kakazu, N., Taguchi, Y., Hirayama, T., Sasaki, A., Hirabayashi, T., Koide, T., Kitsukawa, T., Hamada, S., Yagi, T.
    • Journal Title

      Nature Genetics 37

      Pages: 171-176

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular evolution of Cadherin-related neuronal receptor/protocadherin α(CNR/Pcdha) gene cluster in Mus musculus subspecies2005

    • Author(s)
      Taguchi et al.
    • Journal Title

      Mol.Biol.Evol. 22

      Pages: 1433-1443

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Monoallelic yet combinatorial expression of variable exons of the protocadherin- a gene cluster in single neurons2005

    • Author(s)
      Esumi et al.
    • Journal Title

      Nature Genetics 37

      Pages: 171-176

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Molecular evolution of Cadherin-related neuronal receptor/protocadherin・(CNR/Pcdh・) gene cluster in Mus musculus subspecies.

    • Author(s)
      Taguchi, Y., Koide, T., Shiroishi, T., Yagi, T.
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Length variation of CAG/CAA triplet repeats in 50 genes among 16 inbred mouse strains

    • Author(s)
      Ogasawara et al.
    • Journal Title

      Gene (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Molecular evolution of Cadherin-related neuronal receptor/protocadherin ・(CNR/Pcdh・) gene cluster in Mus musculus subspecies

    • Author(s)
      Taguchi et al.
    • Journal Title

      Mol.Biol.Evol. (In press)

    • Related Report
      2004 Annual Research Report
  • [Book] 痛覚感受性の個体差にかかわる遺伝学的解析.オピオイド研究の進歩と展望(鎮痛薬・オピオイド研究会編)2004

    • Author(s)
      古瀬民生, 小出剛
    • Total Pages
      15
    • Publisher
      ネオメディカル
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 痛覚感受性の個体差にかかわる遺伝学的解析.オピオイド研究の進歩と展望2004

    • Author(s)
      古瀬民生, 小出剛
    • Total Pages
      15
    • Publisher
      ネオメディカル
    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] カプサイシン受容体の活性調節剤、及びそれを用いた医薬組成物2005

    • Inventor(s)
      鈴木誠, 小出剛
    • Industrial Property Rights Holder
      鈴木誠
    • Industrial Property Number
      2005-310000
    • Filing Date
      2005-10-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi