| Project/Area Number |
16310031
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental impact assessment/Environmental policy
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| Research Institution | International Christian University |
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Principal Investigator |
CHIURA Hiroshi International Christian University, CLA, Professor, 教養学部, 教授 (00103698)
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| Co-Investigator(Kenkyū-buntansha) |
RIDGE Robert International Christian University, CLA, Professor, 教養学部, 教授 (10211666)
KAWARABAYASI Yutaka National Institute of Advanced Industrial Science and Technology, Chief Researcher, 糖鎖工学センタ, 主任研究員 (90195165)
KURUSU Yasurou Ibaraki University, College of Agictiture, Professor, 農学部, 教授 (60272118)
SUGIDATE Toshihiro International Christian University, CLA, Researcher, 教養学部, 研究員 (10407190)
帆秋 利洋 大成建設, 土木研究所, 主任研究員
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥15,900,000 (Direct Cost: ¥15,900,000)
Fiscal Year 2006: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2005: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2004: ¥10,200,000 (Direct Cost: ¥10,200,000)
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| Keywords | broad-host range gene transfer particle / gene transfer mechanism in the environment / VLP's / marine environment / thermal environment / Soil / transduction / Aquifex |
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| Research Abstract |
1.Particle production was confirmed from marine α ＆ γ Proteobacteria 22 species, Aquifex 1 specie, and Thermococcus 1 specie. Aquifex sp originating broad-host range gene transfer particle (ST-VP) : ST-VP mediated Escherichia coli transductant (ST-E-trans) produced particle (STEVP) transferred chromosomal genes, plasmid (transduction frequency:〜10^<-6> transductants/particle), and cytoplasmic protein to different phylogenetic recipients. STEVP exhibited enzymatic activities. Introduction of recA^- relA^- mutation led the particle production from the stationary phase to the logarithmic phase, and transduced DNA harboured in the cytoplasm as an extrachromosomal gene.
2.Molecular cloning of VP particle DNA from STEtrans showed almost equal frequency of E.coli chromosomal genes. Horizontal gene transfer by the broad-host range gene transfer particle (VP) was confirmed for the first time with the material realities. Thermophiles and hyperthermophilic archaeon originating VP transferred the h
… More
igh temperature resistance to the mesophilic bacteria.
3.Infection of virus like particle (SG-VLP) from a soil environmental nitrogen fixing bacterium to a receipt, E.coli DH5α, generated the N_2 fixation acquired transductants at the frequency of 4.37 x 10^<-7> transductants/particle. E.coli JE6937 recipient infecting with SG-VLP resulted in subculturable N_2 fixing E.coli at the frequency of ca 10^<-8> transductant/particle with particle production.
4.VLP production was confirmed from environmental SRB.
5.The soluble protein genes of the environmental DNA collection needed for the temperature resistance were expressed in E.coli, whose functions were clarified.
6.C terminal region of eubacterial GroEL showed a positive correlation to the cell proliferation temperature. Single-ring mutant GroEL constructed from Bacillus subtilis groEL applied to identify substrate for B. subtilis and/or E.coli GroE, and gave 28 different species suggesting the diversified substrate binding ability. GroEL binding specificity (B.subtilis and/or E.coli) with B.subtilis RecA protein as a substrate was discovered for the first time. Less
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