| Project/Area Number |
16310087
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Nanomaterials/Nanobioscience
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
YAMAMOTO Takao Osaka University, Graduate School of Engineering, Professor, 大学院工学研究科, 教授 (00174798)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Takashi Tokyo Institute of Technology, Graduate School of Science and Engineering, Associate Professor, 大学院理工学研究科, 助教授 (70273589)
NAKAYAMA Tadachika Nagaoka University of Technology, Extreme Energy-Density Research Institute, Research Associate, 極限エネルギー密度工学研究センター, 助手 (10324849)
SEINO Satoshi Osaka University, Graduate School of Engineering, Assistant Professor, 大学院工学研究科, 講師 (90432517)
MIZUKOSHI Yoshiteru Nagasaki University, Graduate School of Engineering, Research Associate, 工学部, 助手 (60342523)
OKITSU Kenji Osaka Prefecture University, Graduate School of Engineering, Research Associate, 大学院工学研究科, 助手 (60295095)
|
|---|
| Project Period (FY) |
2004 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥15,800,000 (Direct Cost: ¥15,800,000)
Fiscal Year 2006: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2005: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2004: ¥9,000,000 (Direct Cost: ¥9,000,000)
|
|---|
| Keywords | magnetic iron oxide / noble metal / gamma-rays / nanoparticles / biomolecules / magnetic beads / μTAS / thiol base / ナノ粒子 / 磁性粒子 / 磁気分離 / 金被覆 |
|---|
| Research Abstract |
We executed studies for improvement of composite nanoparticles consisting of gold and magnetic iron oxide, in which the former is of single-nano-sized grains and the latter is of larger particles. To synthesize the particles, an aqueous solution containing gold ions, preformed iron oxide nanoparticles, adequate amounts of alcohol and polymer is irradiated with ^<60>Co γ-rays, high energy electrons or ultrasonics. Aqueous gold ions are reduced to form metallic grains by radicals in-situ formed by the irradiation, and the grains are stabilized iron oxide particles after meanwhile migration in water. It is a very simple method proceeding in an aqueous solution at room temperature under ambient atmosphere. We tried many experiments and found conditions providing a good dispersiblity, that they kept a dispersion in water for weeks. This dispersibility was given by a wet beads mill. We as well studied their properties and modifying them for application to a quick and selective separation of biomolecules such as DNA, proteins or antigens, by exploiting Au's high affinity to sulfur-containing molecules and magnetism of the iron oxide. It was already demonstrated that the particles are able to magnetically separate nucleotides with sensitivity higher than those of commercially available particles. We also tried experiments that these particles are charged into a channel with sub-millimeter width. The particle dispersing solution was mixed with an another solution and applied with a magnetic field with a small permanent magnet chip. We successfully confirmed the magnetic particles were attracted by the field to form a dot.
|
