Structural Fluctuation of PhotosyntheticAntenna Complexes Studied by Single-Molecule Spectroscopy
| Project/Area Number |
16340121
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics/Chemical physics
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
MATSUSHITA Michio Tokyo Institute of Technology, Graduate School of Science and Technology, Associate (80260032)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥16,500,000 (Direct Cost: ¥16,500,000)
Fiscal Year 2006: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2004: ¥8,300,000 (Direct Cost: ¥8,300,000)
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| Keywords | protein / single-molecule spectroscopy / structure-function relationship / photosynthetic antenna complex / 光合成 / アンテナ複合体 / LH12 / LH2 |
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| Research Abstract |
A protein has numerous meta-stable structures. In the physiological conditions. The structural fluctuation was pointed out for long years to be important, because the structural fluctuation plays essential role in regulating function of proteins. However, there have been no methods to elucidate the structural dynamics. To break through, we are aiming at getting structural information of single proteins by performing optical spectroscopy of individual proteins. Because measuring a fluorescence spectrum takes at least several milli-seconds, troscopic measurements are performed at temperatures low enough for the structural dynamics to be followed.
In this study we have succeeded to follow one pigment-protein complex to know the temperature dependence of structural dynamics. The experimental data, which are the very first data on the globe show clearly that substantial part of the structural movement is temperature-independent tunneling motion [Oikawa, et. al. J. Am. Chem. Soc. 130(20008)48501.].
Spectroscopy at low temperature of single proteins has been limited to the near infrared region. The method was successfully applied to photosynthetic antenna complexes of photosynthetic bacteria. Nevertheless it is worth making an effort to extend the wavelength of the setup to cover the visible region, since many enzymes absorb or emit in the visible. In order to reduce the chromatic aberration of the objective, which is immersed into liquid helium together with a sample, we have developed a reflecting objective [Fujiyoshi, et. al. Appl. Phys. Lett. 91 (2007) 051125.]. With the reflecting objective we have made two-photon excitation and measured fluorescence spectrum of single GFPs. The spectra were taken in the background-free condition, and series of spectra of the same protein revealed structural dynamics of the protein [Fujiyoshi, et. al. Phys. Rev. Lett. 100 (2008) 168101.].
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Report
(4 results)
Research Products
(67 results)
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[Journal Article] Single-component reflecting objective for low-temperature spectroscopy in the entire visible region2007
Author(s)
S., Fujiyoshi, M., Fujiwara, C., Kim, M., Matsushita, A.M. van, Oijen, J., Schmidt
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Journal Title
Applied Physics Letters 91
Description
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[Journal Article] Probing the Electronic Structure and Conformational Flexibility of Individual Light-Harvesting 3 Complexes by Optical Single-Molecule Spectroscopy2006
Author(s)
M., Ketelaars, J., M. Segura, S., Oellerich, W. P., de Ruijter, G., Magis, T.J., Aartsma, M., Matsushita, J., Schmidt, R.G., Cogdell, J., KOhler
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Journal Title
Journal of Physical Chemistry B 110
Pages: 18710-18717
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Spectroscopic study on photosynthetic antenna complexes in artificial lipid membrane2009
Author(s)
D., Uchiyama, A., Furuya, S., Fujiyoshi, M., Matsushita, T., Dewa, M., Nango
Organizer
The 62nd annual meeting of Physical Society of Japan
Place of Presentation
Sapporo
Year and Date
2009-09-21
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Fluorescence microscopy on energy transfer between photosynthetic antenna complexes2008
Author(s)
A., Furuya, D., Uchiyama, S., Fujiyoshi, M., Matsushita, Y., Suemori, T., Dewa, M., Nango
Organizer
The 63rd annual meeting of Physical Society of Japan
Place of Presentation
Osaka
Year and Date
2008-03-23
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Structure of light-harvesting 2 complex from Rps. acidophila in lipid bilayer of DMPC : A single-molecule study at 5 K2007
Author(s)
D., Uchiyama, H., Oikawa, S., Fujiyoshi, T., Dewa, M., Nango, M., Matsushita
Organizer
The 2nd International Workshop on Photosynthetic Antennae and Coherent Phenomena
Place of Presentation
Osaka
Year and Date
2007-12-15
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Low-temperature fluorescence microscope for two-color excitation of a single protein2007
Author(s)
M., Hirano, M., Fujiwara, S., Fujiyoshi, M., Matsushita, M., Izeki, M., Watanabe
Organizer
The 1st molecular science conference
Place of Presentation
Sendai
Year and Date
2007-09-18
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Temperature dependence of thermal motion of a single protein followed by spectral diffusion of chromophores between 5 - 40K2007
Author(s)
H., Oikawa, S., Fujiyoshi, M., Matsushita, T., Dewa, M., Nango
Organizer
Light-Harvesting Processes
Place of Presentation
Banz, Germany
Year and Date
2007-03-22
Description
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[Presentation] Photosynthetic antenna complexes and Frenkel excitons2006
Author(s)
D., Uchiyama, H., Oikawa, S., Fujiyoshi, M., Matsushita, Y., Suemori, T., Dewa, M., Nango
Organizer
Workshop on "new paradigm of photosynthesis research
Place of Presentation
Osaka
Year and Date
2006-10-12
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Structure of photosynthetic antenna complexes in micelle vs in lipid membrane2006
Author(s)
D., Uchiyama, S., Fujiyoshi, M., Matsushita, Y., Suemori, T., Dewa, M., Nango
Organizer
Autumn meeting of Physical Society of Japan
Place of Presentation
Chiba
Year and Date
2006-09-23
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Low-temperature fluorescence microscope for two-color excitation of a single protein2006
Author(s)
D., Uchiyama, S., Fujiyoshi, M., Matsushita, Y., Suemori, T., Dewa, M., Nango
Organizer
Molecular Structure Conference
Place of Presentation
Shizuoka
Year and Date
2006-09-20
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Spectral diffusion of a single photosynthetic antenna complex at liquid-helium temperature2006
Author(s)
H., Oikawa, S., Fujiyoshi, M., Matsushita, Y., Suemori, T., Dewa, M., Nango
Organizer
The 61st annual meeting of Physical Society of Japan
Place of Presentation
Matsuyama
Year and Date
2006-03-28
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Spectral diffusion of a single photosynthetic antenna complex2005
Author(s)
H., Oikawa, S., Fujiyoshi, M., Matsushita, Y., Suemori, T., Dewa, M., Nango
Organizer
Molecular Structure Conference
Place of Presentation
Tokyo
Year and Date
2005-09-29
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Temperature dependence of the fluorescence excitation spectrum of a single photosynthetic antenna complex between 5 and 40 K2005
Author(s)
H., Oikawa, C., Kim, M., Matsushita, Y., Suemori, T., Dewa, M., Nango
Organizer
The 60th annual meeting of Physical Society of Japan
Place of Presentation
Chiba
Year and Date
2005-03-27
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 固体中のナノプローブとしての単一分子分光2004
Author(s)
松下道雄, アンドレアスブロス, ヤニーク デュラン, エドガー JJ グローネン, ヤン シュミット
Organizer
日本光学会年次学術講演会OJ2004シンポジウム「ナノオプティクス:ナノスケール分光学を目指して」
Place of Presentation
大阪大学コンベンションセンター
Year and Date
2004-11-05
Description
「研究成果報告書概要(和文)」より
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[Presentation] Single-molecule spectroscopy of Light-Harvesting 2 complexes in lipid membrane2004
Author(s)
D., Uchiyama, H., Hoshino, M., Matsushita, Y., Suemori, T., Dewa, M., Nango
Organizer
Molecular Structure Conference
Place of Presentation
Hiroshima
Year and Date
2004-09-28
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Single-molecule spectroscopy of photosynthetic antenna complexes in lipid membrane2004
Author(s)
H., Hoshino, D., Uchiama, M., Matsushita, Y., Suemori, T., Dewa, M., Nango
Organizer
Autumn Meeting of Physical Society of Japan
Place of Presentation
Aomori
Year and Date
2004-09-12
Description
「研究成果報告書概要(欧文)」より
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