| Project/Area Number |
16360207
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Measurement engineering
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
SUEHIRO Junya KYUSHU UNIVERSITY, Graduate School of Information Science and Electrical Engineering, Associate Professor, 大学院・システム情報科学研究院, 助教授 (70206382)
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| Co-Investigator(Kenkyū-buntansha) |
HARA Masanori KYUSHU UNIVERSITY, Professor, 大学院・システム情報科学研究院, 教授 (30039127)
IMASAKA Kiminobu KYUSHU UNIVERSITY, Research Associate, 大学院・システム情報科学研究院, 助手 (40264072)
SENDA Akira Aichi Gakuin University, Department of Dentistry, Professor, 歯学部, 教授 (80097584)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2005: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2004: ¥9,700,000 (Direct Cost: ¥9,700,000)
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| Keywords | Dielectrophoresis / Impedance / Antigen-antibody reaction / Electropermeabilization / Carbon Nanotube / Bacteria inspection / Electrochemical reaction / Escherichia coli |
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| Research Abstract |
Electropermeabilization (EP) was successfully incorporated with DEPIM in order to achieve higher sensitivity for bacteria detection. Contamination of metallic ions released from electrodes, which masks subtle conductance increase caused by the released intracellular ions, was successfully suppressed by improving the pulse waveform for EP. E. coli was quantitatively detected in 3 h at 10^2 CFU/ml, which was improved by two orders of magnitude relative to the conventional DEPIM without EP. The proposed technique is promising as a fast and sensitive bacteria inspection method and may be easily incorporated into Bio-MEMS or μ-TAS devices.
In order to add selectivity to DEPIM or EPA-DEPIM inspection of bacteria, new inspection protocols were demonstrated using an electrode chip on which a target bacteria-specific antibody was immobilized in advance. In the first method, all bacteria were first trapped onto the electrode chip by strong positive DEP. In order to selectively retain the target bacteria, which could bind with the immobilized antigen, the hydrodynamic drag force was exerted by washing liquid flow. Although the number of retained target bacteria increased by using the antibody-modified chip, undesired non-specific binding to the unmodified chip also took place. The second method utilized lower DEP force so that trapping and washing processes could be simultaneously accomplished. A smaller number of retained bacteria under decreased DEP force was surely detected by EPA-DEPIM, which was more sensitive than the conventional DEPIM. It was found that this protocol could also suppress the non-specific bacteria binding and greatly improve the selectivity for the target bacteria.
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