Isolation and characterization of transcription factors controlling quantity and timing of the body wall muscle proteins in C. elegans
| Project/Area Number |
16370005
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Genetics/Genome dynamics
|
|---|
| Research Institution | OKAYAMA UNIVERSITY |
|---|
Principal Investigator |
KAGAWA Hiroaki Okayama University, Graduate School of Natural Science and Technology, Professor, 大学院自然科学研究科, 教授 (10022732)
|
|---|
| Project Period (FY) |
2004 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 2006: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥6,400,000 (Direct Cost: ¥6,400,000)
|
|---|
| Keywords | Caenorhabditis elegans / body wall muscle gene / transcription factor / troponin C / green fluorescence protein / yeast one hybrid system / MyoD / xyxsin / GFP(蛍光蛋白質) / RNAi(RNA干渉法) / ウェスタン解析 / 筋肉潰伝子 |
|---|
| Research Abstract |
MyoD homolog of Caenorhabditis elegans is important but not essential for the body-wall muscle gene expression. Three enhancer sequences of the CeMyoD gene, hlh-1 are isolated in the second intron of the gene. To isolate the regulatory factor of body-wall muscle gene expression, which determines quantity of muscle proteins and time of muscle formation, we performed a modified-yeast one-hybrid screening and isolated LIM-8 and CeTIS-11 that show homology to TRIP6/Zyxin and TIS-11, respectively. LIM-8 interacted with CeMyoD but did not bind to enhancer sequences directly. A series of lim-8::gfp fusion genes were expressed in body-wall, vulval and anal muscles, and other non-muscle tissues. Anti-LIM-8 stained the locus of non-overlapping to DAPI staining DNA region in nucleus of body wall muscles. CeTIS-11 was also located body wall muscles and other tissues. lim-8 (RNAi) or CeTIS-11 (RNAi) worm decreased to 80% or 18% of the mobility of non-treated animal. CeTIS-11 (RNAi) worm also show egg-laying defect that comes from loss function of vulva muscle. This was the difference between LIM-8 bound to CeMyoD and CeTIS-11 directly bound to 1275 enhancer of the CeMyoD gene. These results suggest that LIM-8 regulates the body-wall muscle gene expressions interacting with CeMyoD. We propose an expression control model for the body wall genes.
|
Report
(4 results)
Research Products
(21 results)
-
[Journal Article] Functional importance of polymerization and localization of calsequestrin in C, elegans2007
Author(s)
J.H.Cho, K.M.Ko, G.Singaruvelu, W.H.Lee, G.B.Kang, S.H.Rho, B.J.Park, J.R.Yu, H.Kagawa, S.H.Eom, D.H.Kim, J.H.Ahnn
-
Journal Title
J. Cell Sci. 120
Pages: 1551-1558
Description
「研究成果報告書概要(和文)」より
Related Report
-
-
-
-
[Journal Article] Functional importance of polymerization and localization of calsequestrin in C. elegans2007
Author(s)
Jeong Hoon Cho, Kyung Min Ko, Gunasekaran Singaruvelu, Wonhae Lee, Gil Bu Kang, Seong-HwanRho, Byung-Jae Park, Jae-Ran Yu, Hiroaki Kagawa, Soo Hyun Eom, Do Han Kim, Joohong Ahnn
-
Journal Title
J.Cell Sci. 120
Pages: 1551-1558
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
[Journal Article] elegans model for studying tropomyosin and troponin regulations of muscle contraction and animal behavior. In : S. Ebashi, I. Ohtsuki (Eds.), Regulatory mechanisms of striated muscle contraction.2007
Author(s)
H.Kagawa, T.Takaya, R.Ruksana, F.Anokye-Danso, Md.Z.Amin, H.Terami, C.
-
Journal Title
Adv.Exp.Med. and Biol., Springer, http://escholarshilib.okayama-u.ac.jp/biology general/23/(Tokyo) vol.594
Pages: 153-161
Description
「研究成果報告書概要(欧文)」より
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
