Roles played by jasmonic acid and allene oxide synthase in growth, flowering, and stress responses of rice plants
| Project/Area Number |
16370027
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | Osaka City University |
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Principal Investigator |
IINO Moritoshi Osaka City University, Graduate School of Science, Associate Professor (50176054)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2006: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2005: ¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 2004: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | Jasmonate / Allene Oxide Synthase / Photomorphogenesis / Phytochrome / Wound-induced response / Coleoptiles / Rice / Oryza sativa / 開花 |
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| Research Abstract |
The growth of rice (Oryza sativa) coleoptiles is subject to strong phytochrome-mediated inhibition. The cpm1 mutant of rice (cpm : coleoptile photomorphogenesis) was isolated as a mutant impaired specifically in this photoinhibition and additionally in the process of anthesis. We identified the CPM1 locus by map-based cloning and complementation test to encode a putative allene oxide synthase, OsAOS1. This finding suggested that jasmonates (jasmonic acid and metabolically related substances) are involved in the phytochrome-mediated inhibition of coleoptile growth in addition to their well-known roles in would-induced defense responses. The cpm1 mutant has a point-mutation in the OsAOS1 gene and is expected to express a full-length OsAOS1 in which one amino acid is substituted. This amino acid residue is conserved in all known CYP74 members which include AOSs. Using recombinant proteins, it was confirmed that the AOS activity of OsAOS1 is strongly impaired by the point mutation. It was further confirmed that cpm1 coleoptiles are strongly impaired in red light-induced increases of endogenous JA. These results demonstrated that jasmonates play a major signaling role in phytochrome-mediated inhibition of coleoptile growth. Most of the enzyme gene homologs demonstrated or predicted to be involved in JA biosynthesis are upregulated by red light as well as by wounding. Further analysis with a rice oligo-DNA microarray indicated that expressions of about 3600 genes are regulated commonly by wounding and red light and that at least a half of those genes are regulated in an OsAOS1-dependent manner. Interestingly, this microarray analysis and subsequent analysis by real time RT-PCR indicated that many growth-related genes are regulated (mostly down-regulated) specifically by red light in an OsAOS1-dependent manner. It is suggested that phytochrome-specific jasmonate signaling participates in the phytochrome-mediated inhibition of rice coleoptile growth.
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Report
(4 results)
Research Products
(27 results)
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[Presentation] Jasmonates play a major signaling role in phytochrome-mediated photoinhibition of coleoptile growth in rice seedlings2009
Author(s)
Haga, K., Kiyota, S., Jikumaru, Y., Kamiya, Y., Takano, M., Iino, M.
Organizer
Memorial symposium of the 25th international prize for biology
Place of Presentation
Kyoto
Year and Date
2009-12-12
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Jasmonates play a major signaling role in phytochrome-mediated photoinhibition of coleoptile growth in rice seedlings2009
Author(s)
Haga,K., Kiyota,S., Jikumaru,Y., Kamiya,Y., Takano,M., lino,M.
Organizer
Memorial symposium of the 25th international prize for biology
Place of Presentation
京都大学
Year and Date
2009-12-03
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] Functional analysis of a rice allene oxide synthase gene(OsAOS1)that functions for jasmonate biosynthesis2008
Author(s)
Haga, K., Kiyota, S., Jikumaru, Y., Kamiya, Y., Takano, M., Iino. M
Organizer
The 49th Annual Meeting of the Japanese Society of Plant Physiologists
Place of Presentation
Sapporo
Year and Date
2008-03-22
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Book] Light Sensing in Plants2005
Author(s)
Wada,M., Shimazaki,K., lino,M.(Eds.)
Total Pages
370
Publisher
Springer-Verlag Tokyo
Description
「研究成果報告書概要(和文)」より
Related Report
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