Molecular mechanisms of synthesis, transport, and accumulation of glycinebetaine in plants and cyanobacteria

• Project/Area Number: 16370028
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 植物生理・分子
• Research Institution: Meijo University
• Principal Investigator: TAKABE Teruhiro Meijo University, Research Institute, Professor, 総合研究所, 教授 (80097766)
• Co-Investigator(Kenkyū-buntansha): TANAKA Yoshito Meijo University, Faculty of Science & Technology, Associate Professor, 理工学部, 助教授 (10247679) ; HIBINO Takashi Meijo University, Faculty of Science & Technology, Associate Professor, 理工学部, 助教授 (70218741) ; NAKAMURA Tatsunosuke Niigata University, Pharmacy & Applied Life Science, Professor, 薬学部, 教授 (20114308) ; SHIKATA Masamitsu Shimadzu Co., Genomic research, Director, 分析計測事業部ジェノミックリサーチ室, 副主任研究員 (20374126)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥15,300,000 (Direct Cost: ¥15,300,000); Fiscal Year 2006: ¥5,300,000 (Direct Cost: ¥5,300,000); Fiscal Year 2005: ¥5,600,000 (Direct Cost: ¥5,600,000); Fiscal Year 2004: ¥4,400,000 (Direct Cost: ¥4,400,000)
• Keywords: Grovel environment / Food / Salt stress / Glycinebetaine / Genetic engineering / Plants / Halotolerant cyanobacterium / Transport

Research Abstract

Glycine betaine (betaine) is a important osmoprotectant which accumulates under abiotic stresses. In this projects, we have studied molecular mechanisms of synthesis, transport and accumulation of betaine in plantas and halotolerant cyanobacteria. In this project, we obtained the following results. 1) Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in Synechococcus and Arabidopsis. 2) Isolation and functional characterization of Ca^<2+>/H^+ antiporters from cyanobacteria. 3) Isolation and functional characterization of betaine transporters from halotolerant cyanobacterium and mangrove. 4) Cloning, functional expression and primary characterization of Vibrio parahaemolyticus K^+/H^+ antiporter Escherichia coli ChaA. 4) Halotolerant cyanobacterium Aphanothece halophytica contains a betaine transporter active at alkaline pH and high salinity. 5) Carboxyl-terminal hydrophilic tail of a NhaP type Na^+/H^+ antiporter from cyanobacteria is involved in the apparent affinity for Na^+ and pH sensitivity. 6) Root specific expression of Na^+/H^+ antiporter gene from Synechosystis sp. PCC6803 confers salt tolerance of tobacco plant.

Research Products

• [Journal Article] Cloning, functional expression and primary characterization of Vibrio parahaemolyticus K^+/H^+ antiporter genes in Escherichia coil. (2006)
  • Author(s): Radchenko MV, Waditee R, Oshimi S, Fukuhara M, Takabe T, Nakamura T
  • Journal Title: Mol. Microbiol. 59 Pages: 651-663
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Potassium/proton antiport system of Escherichia coli. (2006)
  • Author(s): Radchenko MV, Tanaka K, Waditee R, Oshimi S, Matsuzaki Y, Fukuhara M, Kobayashi H, Takabe T, Nakamura T
  • Journal Title: J. Biol. Chem. 281 Pages: 19822-19829
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Halotolerant cyanobacterium Aphanothece halophytica contains a betaine transporter active at alkaline pH and high salinity. (2006)
  • Author(s): Laloknam S, Tanaka K, Buaboocha T, Waditee R, Incharoensakdi A, Hibino T, Tanaka Y, Takabe T
  • Journal Title: Appl. Environ. Microbiol 72 Pages: 6018-6026
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Carboxyl-terminal hydrophilic tail of a NhaP type Na^+/H^+ antiporter from cyanobacteria is involved in the apparent affinity for Na+ and pH sensitivity. (2006)
  • Author(s): Waditee R, Buaboocha T, Kato M, Hibino T, Suzuki S, Nakamura T, Takabe T
  • Journal Title: Arch. Biochem. Biophys. 450 Pages: 113-121
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Root specific expression of Na^+/H^+ antiporter gene from Synechosystis sp. PCC6803 confers salt tolerance of tobacco plant. (2006)
  • Author(s): Hossain GS, Waditee R, Hibino T, Tanaka Y, Takabe T
  • Journal Title: Plant Biotechnol 23 Pages: 275-281
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Conformational changes during apoplastocyanin folding observed by photocleavable modification and transient grating. (2006)
  • Author(s): Hirota S, Fujimoto Y, Choi J, Baden N, Katagiri N, Akiyama M, Hulsker R, Ubbink M, Okajima T, Takabe T, Funasaki N, Watanabe Y, Terazima M
  • Journal Title: J. Am. Chem. Soc. 128 Pages: 7551-7558
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Reduction of plastocyanin by tyrosine-containing oligopeptides. (2006)
  • Author(s): Hirota S, Okumura H, Kondoh T, Funasaki N, Takabe T, Watanabe Y
  • Journal Title: J. Inorg. Biochem 100 Pages: 1871-1878
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Potassium/proton antiport system of Escherichia coli (2006)
  • Author(s): Radchenko MV, Tanaka K, Waditee R, Oshimi S, Matsuzaki Y, Fukuhara M, Kobayashi H, Takabe T, Nakamura T.
  • Journal Title: J.Biol.Chem. 281 Pages: 19822-19829
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Halotolerant cyanobacterium Aphanothece halophytica contains a betaine transporter active at alkaline pH and high salinity (2006)
  • Author(s): Laloknam S, Tanaka K, Buaboocha T, Waditee R, Incharoensakdi A, Hibino T, Tanaka Y, Takabe T.
  • Journal Title: Appl.Environ.Microbiol. 72 Pages: 6018-6026
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Carboxyl-terminal hydrophilic tail of a NhaP type Na^+/H^+ antiporter from cyanobacteria is involved in the apparent affinity for Na+ and pH sensitivity (2006)
  • Author(s): Wditee R, Buaboocha T, Kato M, Hibino T, Suzuki S, Nakamura T, Takabe T.
  • Journal Title: Arch.Biochem.Biophys. 450 Pages: 113-121
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Root specific expression of Na^+/H^+ antiporter gene from Synechosystis sp. PCC6803 confers salt tolerance of tobacco plant (2006)
  • Author(s): Hossain GS, Waditee R, Hibino T, Tanaka Y, Takabe T.
  • Journal Title: Plant Biotechnol. 23 Pages: 275-281
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cloning, functional expression and primary characterization of Vibrio parahaemolyticus K^+/H^+ antiporter genes in Escherichia coli. (2006)
  • Author(s): Radchenko MV, Waditee R, Oshimi S, Fukuhara M, Takabe T, Nakamura T.
  • Journal Title: Mol.Microbiol. 59 Pages: 651-663
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Conformational changes during apoplastocyanin folding observed by photocleavable modification and transient grating (2006)
  • Author(s): Hirota S, Fujimoto Y, Choi J, Baden N, Katagiri N, Akiyama M, Hulsker R, Ubbink M, Okajima T, Takabe T, Funasaki N, Watanabe Y, Terazima M.
  • Journal Title: J.Am.Chem.Soc. 128 Pages: 7551-7558
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Reduction of plastocyanin by tyrosine-containing oligopeptides (2006)
  • Author(s): Hirota S, Okumura H, Kondoh T, Funasaki N, Takabe T, Watanabe Y.
  • Journal Title: J.Inorg.Biochem. 100 Pages: 1871-1878
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cloning, functional expression and primary characterization of Vibrio parahaemolyticus K~+/H~+ antiporter genes in Escherichia coli. (2006)
  • Author(s): Radchenko MV, Waditee R, Oshimi S, Fukuhara M, Takabe T, Nakamura T.
  • Journal Title: Mol.Microbiol. 59 Pages: 651-663
• [Journal Article] Potassium/proton antiport system of Eschericia coli. (2006)
  • Author(s): Radchenko MV, Tanaka K, Waditee r, Oshimi S, Matsuzaki Y, Fukuhara M, Kobayashi H, Takabe T, Nakamura T.
  • Journal Title: J.Biol.Chem. 281 Pages: 19822-19829
• [Journal Article] Halotolerant cyanobacterium Aphanothece halophytica contains a betaine transporter active at alkaline pH and high salinity. (2006)
  • Author(s): Laloknam S, Tanaka K, Buaboocha T, Waditee R, Incharoensakdi A, Hibino T, Tanaka Y, Takabe T.
  • Journal Title: Appl.Environ.Microbiol 72 Pages: 6018-6026
• [Journal Article] Carboxyl-terminal hydrophilic tail of a NhaP type Na~+/H~+ antiporter from cyanobacteria is involved in the apparent affinity for Na+ and pH sensitivity. (2006)
  • Author(s): Waditee R, Buaboocha T, Kato M, Hibino T, Suzuki S, Nakamura T, Takabe T.
  • Journal Title: Arch.Biochem.Biophys. 450 Pages: 113-121
• [Journal Article] Root specific expression of Na~+/H~+ antiporter gene from Synechosystis sp. PCC6803 confers salt tolerance of tobacco plant. (2006)
  • Author(s): Hossain GS, Waditee R, Hibino T, Tanaka Y, Takabe T.
  • Journal Title: Plant Biotechnol 23 Pages: 275-281
• [Journal Article] Conformational changes during apoplastocyanin folding observed by photocleavable modification and transient grating. (2006)
  • Author(s): Hirota S, Fujimoto Y, Choi J, Baden N, Katagiri N, Akiyama M, Hulsker R, Ubbink M, Okajima T, Takabe T, Funasaki N, Watanabe Y, Terazima M.
  • Journal Title: J.Am.Chem.Soc. 128 Pages: 7551-7558
• [Journal Article] Reduction of plastocyanin by tyrosine-containing oligopeptides. (2006)
  • Author(s): Hirota S, Okumura H, Kondoh T, Funasaki N, Takabe T, Watanabe Y.
  • Journal Title: J.Inorg.Biochem 10 Pages: 1871-1878
• [Journal Article] Cloning, functional expression, and primary characterization of Vibrio parahaemolyticus K^+/H^+ antiporter genes in Escherichia coli (2006)
  • Author(s): Radchenko MV, Waditee R, Oshimi S, Fukuhara M, Takabe T, Nakamur T
  • Journal Title: Mol.Microbiol. 59 Pages: 651-663
• [Journal Article] Root specific expression of Na^+/H^+ antiporter gene from Synechocystis sp.PCC 6803 confers salt tolerance of tobacco plant (2006)
  • Author(s): Hossain G, Waditee R, Hibino T, Tanaka Y, Takabe T
  • Journal Title: Plant Biotechnol (In press)
• [Journal Article] Carboxyl-terminal hydrophilic tail of a NhaP type Na^+/H^+ antiporter from cyanobacteria is involved in the apparent affinity for Na^+ and pH sensitivity (2006)
  • Author(s): WaditeeR, BuaboochaT, Kato M, Hibino T, Suzuki S, Nakamura T, Takabe T
  • Journal Title: Arch Biophys Biochem (In press)
• [Journal Article] Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in Synechococcus and Arabidopsis (2005)
  • Author(s): Waditee R, Bhuiyan MN, Rai V, Aoki K, Tanaka Y, Hibino T, Suzuki S, Takano J, Jagendorf AT, Takabe T, Takabe T
  • Journal Title: Proc. Natl. Acad. Sci. USA 102 Pages: 1318-1323
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Halotolerant cyanobacterium Aphanothece halophytica contains NapA-type Na^+/H^+ antiporters with novel ion specificity that are involved in salt tolerance at alkaline pH (2005)
  • Author(s): Wutipraditkul, N., Waditee, R., Incharoensakdi, A., Hibino, T., Tanaka, Y., Nakamura, T., Shikata, M., Takabe, T., Takabe, T.
  • Journal Title: Appl. Environ. Microbiol. 71 Pages: 4176-4184
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Functional differences between cyanobacterial DnaK1 chaperones from the halophyte Aphanothece halophytica and the freshwater species Synechococcus elongatus expressed in Escherichia coli (2005)
  • Author(s): Blanco-Rivero MC, Takabe T, Viale AM
  • Journal Title: Curr. Microbiol. 51 Pages: 164-170
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Suppressed expression of apoplastic ascorbate oxidase gene increases salt tolerance in tobacco and Arabidopsis plants (2005)
  • Author(s): Yamamoto, A., Bhuiyan, M.N., Waditee, R., Tanaka, Y., Esaka, M., Oba, K., Jagendorf, A.T., Takabe, T.
  • Journal Title: J. Exp. Bot. 56 Pages: 1785-1796
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] All four putative selectivity filter glycine residues in KtrB are essential for high affinity and selective K+ uptake by the KtrAB system from Vibrio alginolyticus (2005)
  • Author(s): Tholema N, Vor der Bruggen M, Maser P, Nakamura T, Schroeder JI, Kobayashi H, Uozumi N, Bakker EP
  • Journal Title: J. Biol. Chem. 280 Pages: 41146-41154
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in Synechococcus and Arabidopsis (2005)
  • Author(s): Waditee R, Bhuiyan MN, Rai V, Aoki K, Tanaka Y, Hibino T, Suzuki S, Takano J, Jagendorf AT, Takabe T, Takabe T.
  • Journal Title: Proc.Natl.Acad.Sci.USA 102 Pages: 1318-1323
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Halotolerant cyanobacterium Aphanothece halophytica contains NapA-type Na^+/H^+ antiporters with novel ion specificity that are involved in salt tolerance at alkaline pH (2005)
  • Author(s): Wutipraditkul, N., Waditee, R., Incharoensakdi, A., Hibino, T., Tanaka, Y, Nakamura, T., Shikata, M., Takabe, T., Takabe, T.
  • Journal Title: Appl.Environ.Microbiol. 71 Pages: 4176-4184
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functional differences between cyanobacterial DnaK1 chaperones from the halophyte Aphanothece halophytica and the freshwater species Synechococcus elongatus expressed in Escherichia coli (2005)
  • Author(s): Blanco-Rivero MC, Takabe T, Viale AM.
  • Journal Title: Curr.Microbiol. 51 Pages: 164-170
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Suppressed expression of apoplastic ascorbate oxidase gene increases salt tolerance in tobacco and Arabidopsis plants (2005)
  • Author(s): Yamamoto, A., Bhuiyan, M.N., Waditee, R., Tanaka, Y., Esaka, M., Oba, K., Jagendorf, A.T., Takabe, T.
  • Journal Title: J.Exp.Bot. 56 Pages: 1785-1796
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] All four putative selectivity filter glycine residues in KtrB are essential for high affinity and selective K+ uptake by the KtrAB system from Vibrio alginolyticus (2005)
  • Author(s): Tholema N, Vor der Bruggen M, Maser P, Nakamura T, Schroeder JI, Kobayashi H, Uozumi N, Bakker EP.
  • Journal Title: J.Biol.Chem. 280 Pages: 41146-41154
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in Synechococcus and Arabidopsis (2005)
  • Author(s): Waditee R, Bhuiyan MN, Rai V, Aoki K, Tanaka Y, Hibino T, Suzuki S, Takano J, Jagendorf AT, Takabe T, Takabe
  • Journal Title: Proc Natl Acad Sci U S A. 102 Pages: 1318-1323
• [Journal Article] The Halotolerant Cyanobacterium Aphanothece halophytica, Contains NapA Type Na+/H+ Antiporters Involved in Salt Tolerance at Alkaline pH with Novel Ion Specificity (2005)
  • Author(s): Wutipraditkul N, Waditee R, Incharoensakdi A, Hibino T, Tanaka Y, Nakamura T, Shikata M, Takabe T, Takabe T
  • Journal Title: Appl.Environ.Microb. 71 Pages: 4176-4184
• [Journal Article] Suppressed expression of apoplastic ascorbate oxidase gene increases salt tolerance in tobacco and Arabidopsis plants (2005)
  • Author(s): Yamamoto A, Bhuiyan MN, Waditee R, Tanaka Y, Esaka M, Oba K, Jagendorf AT, Takabe T
  • Journal Title: J.Exp.Bot 56 Pages: 1785-1796
• [Journal Article] Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in Synechococcus and Arabidopsis (2005)
  • Author(s): Waditee R, Bhuiyan MN, Rai V, Aoki K, Tanaka Y, Hibino T, Suzuki S, Takano J, Jagendorf AT, Takabe T, Takabe
  • Journal Title: Proc Natl Acad Sci USA. 102 Pages: 1318-1323
• [Journal Article] The Halotolerant Cyanobacterium Aphanothece halophytica, Contains NapA Type Na+/H+ Antiporters, Involved in Salt Tolerance at Alkaline pH with Novel Ion Specificity (2005)
  • Author(s): Wutipraditkul N, Waditee R, Incharoensakdi A, Hibino T, Tanaka Y, Nakamura T, Shikata M, Takabe T, Takabe T
  • Journal Title: Appl.Environ.Microb. 70
• [Journal Article] アマモ群落の再生産維持機構の解析におけるマイクロサテライトマーカーを用いた個体識別法 (2005)
  • Author(s): 吉田 司, Chunjie TIAN, 岡本英明, 田中義人, 芝修一, 鈴木輝明, 高倍昭洋
  • Journal Title: 水産工学
• [Journal Article] Isolation and functional characterization of Ca^<2+>/H^+ antiporters from cyanobacteria (2004)
  • Author(s): Waditee R, Hossain GS, Tanaka Y, Nakamura T, Shikata M, Takano J, Takabe T, Takabe T
  • Journal Title: J. Biol. Chem. 279 Pages: 2213-2216
  • NAID: 130006988087
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Interaction of plastocyanin with oligopeptides : effect of lysine distribution within the peptide (2004)
  • Author(s): Hirota S, Okumura H, Arie S, Tanaka K, Shionoya M, Takabe T, Funasaki N, Watanabe Y
  • Journal Title: J. Inorg. Biochem. 98 Pages: 849-856
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Isolation and functional characterization of Ca^<2+>/H^+ antiporters from cyanobacteria (2004)
  • Author(s): Waditee R, Hossain GS, Tanaka Y, Nakamura T, Shikata M, Takano J, Takabe T, Takabe T.
  • Journal Title: J.Biol.Chem. 279 Pages: 2213-2218
  • NAID: 130006988087
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Interaction of plastocyanin with oligopeptides : effect of lysine distribution within the peptide (2004)
  • Author(s): Hirota S, Okumura H, Arie S, Tanaka K, Shionoya M, Takabe T, Funasaki N, Watanabe Y.
  • Journal Title: J.Inorg.Biochem. 98 Pages: 849-855
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Isolation and functional characterization of Ca2+/H+ antiporters from cyanobacteria. (2004)
  • Author(s): Waditee R, Hossain GS, Tanaka Y, Nakamura T, Shikata M, Takano J, Takabe T, Takabe T
  • Journal Title: J.Biol.Chem. 278 Pages: 2213-2218
  • NAID: 130006988087
• [Book] Abiotic stress tolerance in plants (2006)
  • Author(s): Rai AK, Takabe T
  • Publisher: Springer
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Metabolic engineering of glycinebetaine. (2006)
  • Author(s): Takabe T, Rai V, Hibino T
  • Publisher: Springer
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Metabolic engineering of glycinebetaine. (2006)
  • Author(s): Waditee R, Tanaka Y, Takabe T
  • Publisher: Springer
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Abiotic stress tolerance in plants (2006)
  • Author(s): Rai AK, Takabe T. (book eds)
  • Publisher: Springer
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Metabolic engineering of glycinebetaine. In Abiotic stress tolerance in plants (2006)
  • Author(s): Takabe T, Rai V, Hibino T.
  • Publisher: Springer
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Na^+/H^+ antiporters in plants and cyanobacteria. In Abiotic stress tolerance in plants (2006)
  • Author(s): Waditee R, Tanaka Y, Takabe T.
  • Publisher: Springer
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Metabolic engineering of glycinebetaine. (2006)
  • Author(s): Takabe T, Rai V, Hibino T.
  • Publisher: Springer