Structure-function relationships of a yeast putative stretch-activated calcium channel

• Project/Area Number: 16370072
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Tokyo Gakugei University
• Principal Investigator: IIDA Hidetoshi Tokyo Gakugei University, Department of Education, Professor, 教育学部, 教授 (70124435)
• Co-Investigator(Kenkyū-buntansha): IIDA Kazuko Tokyo Metropolitan Organization for Medical Research, The Tokyo Metropolitan Institute of Medical Science, Senior Researcher, 東京都臨床医学総合研究所, 主任研究員 (40151229)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥15,400,000 (Direct Cost: ¥15,400,000); Fiscal Year 2006: ¥4,500,000 (Direct Cost: ¥4,500,000); Fiscal Year 2005: ¥5,300,000 (Direct Cost: ¥5,300,000); Fiscal Year 2004: ¥5,600,000 (Direct Cost: ¥5,600,000)
• Keywords: Stretch-activated calcium channel / Calcium channel / Signal transduction / Saccharomyces cerevisiae / Arabidopsis thaliana / Calcium / Mid1 / Cch1 / Mca1 / 機械刺激 / イネ / Spa2 / OsTpc1 / 電位作動型カルシウムチャネル / 伸展活性化Ca^<2+>チャネル / 機械受容チャネル / Ca^<2+>チャネル / 電立依存性Ca^<2+>チャネル

Research Abstract

1) The amino acid residue Phe^<356> of the putative stretch-activated Ca^<2+> channel Mid1 was determined to be important for cell viability in yeast. Systematic substitution of various amino acids for Phe^<355> revealed that hydrophobicity of this residue is critical.
2) The Mid1 protein was found to be localized to the plasma membrane and endoplasmic reticulum membrane. Mid1 forms a oligomer via disulfide bonding.
3) A multicopy suppressor of the mid1 mutation was isolated and characterized. This multicopy suppressor is an N-terminally truncated form of the Spa2 protein important for polarized morphgenesis.
4) A gene encoding Cch1, which has been genetically suggested to function with Mid1 as a Ca^<2+> channel, was cloned. We have confirmed that Mid1 and Cch1 really cooperate to function as a Ca^<2+> channel.
5) Rice OsTpc1 that is homologous to yeast Cch1 was found to be sensitive to blockers of L-type voltage-gated calcium channels. In addition, The Arabidopsis Mca1 protein, which can complement the lethality of the yeast mid1 mutant, was isolated and characterized. Mca1 is crucial for Ca^<2+> influx and touch sensing in primary roots.

Research Products

• [Journal Article] Arabidopsis plasma membrane protein crucial for Ca^<2+> influx and touch sensing in roots (2007)
  • Author(s): Nakagawa, Y., Katagiri, T., Shinozaki, K., Qi, Z., Tatsumi, H., Furuichi, T., Kishigami, A., Sokabe, M., Kojima, I., Sato, S., Kato, T., Tabata, S., Iida, K., Terashima, A., Nakano, M., Ikeda, M., Yamanaka, T., Iida.H.
  • Journal Title: Proc. Natl. Acad. Sci. USA 104 Pages: 3639-3644
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Arabidopsis plasma membrane protein crucial for Ca^<2+> influx and touch sensing in roots. (2007)
  • Author(s): Nakagawa, Y., Katagiri, T., Shinozaki, K., Qi, Z., Tatsumi, H., Furuichi, T., Kishigami, A., Sokabe, M., Kojima, I., Sato, S., Kato, T., Tabata, S., Iida, K., Terashima, A., Nakano, M., Ikeda, M., Yamanaka, T., Iida H.
  • Journal Title: Proc.Natl.Acad.Sci.USA 104 Pages: 3639-3644
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Arabidopsis plasma membrane protein crucial for Ca^<2+>influx and touch sensing in roots (2007)
  • Author(s): Nakagawa, Y., Katagiri, T., Shinozaki, K., Qi, Z., Tatsumi, H., Furuichi, T., Kishigami, A., Sokabe, M., Kojima, I., Sato, S., Kato, T., Tabata, S., Iida, K., Terashima, A., Nakano, M., Ikeda, M., Yamanaka,T., Iida,H.
  • Journal Title: Proc.Natl.Acad.Sci.USA 104 Pages: 3639-3644
• [Journal Article] The polarized morphogenesis regulator Spa2 is required for the function of the putative stretch-activated Ca2+-permeable channel component Mid1 in Saccharomyces cerevisiae (2005)
  • Author(s): Noma, S., Iida, K., Iida, H.
  • Journal Title: Eukaryot. Cell 4 Pages: 1353-1363
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Evidence for the plasma membrane localization of a putative voltage-dependent Ca^<2+> channel, OsTPC1, in rice. (2005)
  • Author(s): Hashimoto, K., Saito, M., Iida, H., Matsuoka, H
  • Journal Title: Plant Biotechnol. 22 Pages: 235-239
  • NAID: 10028053758
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of functional domains of Mid1, a stretch-activated channel component, necessary for localization to the plasma membrane and Ca^<2+> permeation (2005)
  • Author(s): Ozeki-Miyawaki, C., Moriya, Y., Tatsumi, H., Iida, H., Sokabe, M.
  • Journal Title: Exp. Cell Res. 311 Pages: 84-95
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of functional domains of Mid1, stretch-activated channel component, necessary for localization to the plasma membrane and Ca^<2+> permeation. (2005)
  • Author(s): Ozeki-Miyawaki, C., Moriya, Y., Tatsumi, H., Iida H., Sokabe, M.
  • Journal Title: Exp.Cell Res. 311 Pages: 84-95
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Polarize morphogenesis regulator Spa2 is required for the function of putative stretch-activated Ca^<2+>-permeable channel component Mid1 in Saccharomyces cerevisiae. (2005)
  • Author(s): Noma, S., Iida, K., Iida.H.
  • Journal Title: Eukaryot.Cell 4 Pages: 1353-1363
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Evidence for the plasma membrane localization of a putative voltage-dependent Ca^<2+> channel, in rice. (2005)
  • Author(s): Hashimoto, K., Saito, M., Iida H., Matsuoka, H.
  • Journal Title: Plant Biotechnol. 22 Pages: 235-239
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Polarized morphogenesis regulator Spa2 is required for the function of putative stretch-activated Ca2+-permeable channel component Mid1 in Saccharomyces cerevisiae (2005)
  • Author(s): Noma, S., Iida, K., Iida, H.
  • Journal Title: Eukaryotic Cell 4 Pages: 1353-1363
• [Journal Article] Evidence for the plasma membrane localization of a putative voltage-dependent Ca2+ channel, OsTPC1, in rice (2005)
  • Author(s): Hashimoto, K., Saito, M., Iida, H., Matsuoka, H.
  • Journal Title: Plant Biotechnology 22 Pages: 235-239
  • NAID: 10028053758
• [Journal Article] Identification of functional domains of Mid1, a stretch-activated channel component, necessary for localization to the plasma membrane and Ca2+ permeation (2005)
  • Author(s): Ozeki-Miyawaki, C., Moriya, Y., Tatsumi, H., Iida, H., Sokabe, M.
  • Journal Title: Experimental Cell Research 311 Pages: 84-95
• [Journal Article] Phe356 in the yeast Ca2+ channel component Mid1 is a key residue for viability after exposeure to □-factor (2004)
  • Author(s): Tada, T., Ohmori, M., Iida, H.
  • Journal Title: Biochem. Biophs. Res. Commun 313 Pages: 752-757
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Subcellular localization and oligomeric structure of the yeast putative stretch-activated Ca2+ channel component Mid1 (2004)
  • Author(s): Yoshimura, H., Tada, T., Iida, H
  • Journal Title: Exp. Cell Res. 293 Pages: 185-195
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Functional analysis of a rice putative voltage-dependent Ca2+ channel, OsTPC1, expressed in yeast cells lacking its homologous gene CCH1 (2004)
  • Author(s): Hashimoto K., Saito, M., Matsuoka, H., Iida, K., Iida, H.
  • Journal Title: Plant Cell Physiol. 45 Pages: 496-500
  • NAID: 10012852684
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A mechanosensitive anion channel in Arabidopsis thaliana mesophyll cells (2004)
  • Author(s): Qi, Z., Kishigami, A., Nakagawa, Y., Iida, H., Sokabe, M.
  • Journal Title: Plant Cell Physiol. 45 Pages: 1704-1708
  • NAID: 10014020982
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular cloning in yeast by in vivo homologous recombination of the yeast putative □1 subunit of the voltage-gated calcium channel (2004)
  • Author(s): Iida, K., Tada, T., Iida, H.
  • Journal Title: FEBS Lett. 576 Pages: 291-296
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Subcellular localization and oligomeric structure of the yeast putative stretch-activated Ca^<2+> channel component Mid1. (2004)
  • Author(s): Yoshimura, H., Tada, T., Iida, H.
  • Journal Title: Exp.Cell Res. 293 Pages: 185-195
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Phe^<356> in the yeast Ca^<2+> channel comopnent Mid1 is a key residue for viability after exposure to α-factor. (2004)
  • Author(s): Tada, T., Ohmori, M., Iida H.
  • Journal Title: Biochem.Biophys.Res.Commun. 313 Pages: 752-757
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functional analysis of a rice putative voltage-dependent Ca^<2+> channel, OsTPC1, expressed in yeast cells lacking its homologous gene CCH1. (2004)
  • Author(s): Hashimoto, K., Saito, M., Matsuoka, H., Iida, K., Iida H.
  • Journal Title: Plant Cell Physiol. 45 Pages: 496-500
  • NAID: 10012852684
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular cloning in yeast by in vivo homologous recombination of the yeast putative α1 subunit of the voltage-gated calcium channel. (2004)
  • Author(s): Iida, K., Tada, T., Iida H.
  • Journal Title: FEBS Lett. 576 Pages: 1704-1708
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A mechanosensitive anion channel in Arabidopsis thaliana mesophyll.cells. (2004)
  • Author(s): Qi, Z., Kishigami, A., Nakagawa, Y., Iida, H., Sokabe, M.
  • Journal Title: Plant Cell Physiol. 45 Pages: 1704-1708
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Phe356 in the yeast Ca^<2+> channel component Mid1 is a key residue for viability after exposure to α-factor (2004)
  • Author(s): Tada, T., Ohmori, M., Iida, H.
  • Journal Title: J.Biol.Chem. 313 Pages: 752-757
• [Journal Article] Subcellular localization and oligomeric structure of the yeast putative stretch-activated Ca^<2+> channel component Mid1 (2004)
  • Author(s): Yoshimura, H., Tada, T., Iida, H.
  • Journal Title: Exp.Cell Res 293 Pages: 185-195
• [Journal Article] Functional analysis of a rice putative voltage-dependent Ca^<2+> channel, OsTPC1, expressed in yeast cells lacking its homologous gene CCH1. (2004)
  • Author(s): Hashimoto, K., Saito, M., Matsuoka, H., Iida, K., Iida, H.
  • Journal Title: Plant Cell Physiol. 45 Pages: 496-500
  • NAID: 10012852684
• [Journal Article] Molecular cloning in yeast by in vivo homologous recombination of the yeast putative α1 subunit of the voltage-gated calcium channel. (2004)
  • Author(s): Iida, K., Tada, T., Iida, H.
  • Journal Title: FEBS Lett. 576 Pages: 291-296
• [Journal Article] A mechanosensitive anion channel in Arabidopsis thaliana mesophyll cells. (2004)
  • Author(s): Qi, Z., Kishigami, A., Nakagawa, Y., Iida, H., Sokabe, M.
  • Journal Title: Plant Cell Physiol. 45 Pages: 1704-1708
  • NAID: 10014020982
• [Book] 植物の環境応答と形態形成のクロストーク (2004)
  • Author(s): 飯田秀利
  • Total Pages: 9
• [Book] カルシウム動員の測定法-fura-2を中心として- (2004)
  • Author(s): 飯田秀利
  • Total Pages: 9
  • Publisher: 廣川書店
• [Book] 細胞内pH測定法 (2004)
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  • Publisher: 廣川書店