Clarification of energy transduction of bacterial flagellar motor using hybrid bacteria
| Project/Area Number |
16370073
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | TOHOKU UNIVERSITY (2006)
Nagoya University (2004-2005) |
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Principal Investigator |
ISHIJIMA Akihiko Tohoku University, Institute of Multidisciplinary Research for Advanced Materials, Professor, 多元物質科学研究所, 教授 (80301216)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥15,600,000 (Direct Cost: ¥15,600,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2005: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2004: ¥9,400,000 (Direct Cost: ¥9,400,000)
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| Keywords | bacteria / nano measurement / moter / single molecule measurement / ion / I分子計測 / べん毛モーター / キメラ菌体 / イオンモーター / ステップ計測 / ナノテクノロジー / キメラ |
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| Research Abstract |
Bacterial flagellar motor is known as a molecular machine of bacteria, of which diameter is about 40 nm. The driving force is a difference of ion concentration, proton and sodium, between the inside and outside of the cell. We have made the chimera cell using these cells. This cell is suitable for a rotational measurement, plays an important role for elucidation of this mechanism. We succeeded in a measurement of step-size motion of rotation for the first time (Nature, 2005). However, it was not yet confirmed about a basic property of this chimera cell. Therefore we tried to clarify a basic property of this chimera cell. As a result, the torque-speed relations of a chimera cell had the similar characteristics of two motors. Furthermore, stepwise increase of the rotational speed with an incorporation of stator complex was observed, and the torque-speed relationship by one stator complex could be measured.
In addition, imaging technology in a cell is necessary to clarify the information in bacteria cell, the construction situation of a motor complex and a rotational motion a motor. There are several problems about the imaging technology using bacteria cell compared to the normal method, by small and shape of cells. We optimized evanescent illumination and tried to observe of GFP fused protein in bacteria cell. As a result, we succeeded in observing the diffusional motion of GFP fused FliG protein which is one of the motor proteins. Furthermore, we succeeded in estimating the diffusion constant of the fluorescent spots. In addition, we were able to make clear that the position of the GFP fused FliG protein coincided with a pivot point of a cell using a tethered cell assay method.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Formation and maintenance of tubular membrane projections require mechanical force, but their elongation and shortening do not require additional force.2005
Author(s)
Inaba, T., Ishijima, A., Honda, M., Nomura, F., Takiguchi, K., Hotani, H.
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Journal Title
Journal of Molecular Biology 348
Pages: 325-333
Related Report
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[Journal Article] Direct observation of steps in rotation of the bacterial flagellar motor.2005
Author(s)
Sowa, Y., Rowe, A., Leake, M., Yakushi, T., Homma, M., Ishijima, A., Berry, R.
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Journal Title
Nature 437
Pages: 916-919
Related Report
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