Functional analysis of NB-LRR gene family for plant immunity

• Project/Area Number: 16380031
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Tohoku University
• Principal Investigator: TAKAHASHI Hideki Tohoku University, Graduate School of Agricultural Science, Associate Professor, 大学院農学研究科, 助教授 (20197164)
• Co-Investigator(Kenkyū-buntansha): HASE Shu Tohoku University, Graduate School of Agricultural Science, Assistant Professor, 大学院農学研究科, 助手 (10261497)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥14,600,000 (Direct Cost: ¥14,600,000); Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000); Fiscal Year 2005: ¥2,900,000 (Direct Cost: ¥2,900,000); Fiscal Year 2004: ¥9,700,000 (Direct Cost: ¥9,700,000)
• Keywords: Resistance gene / Hypersensitive response / Signal transduction / Cucumber mosaic virus / Plant / キュウリモザイクテスト

Research Abstract

We have isolated Cucumber mosaic virus (CMV) resistance gene RCY1 encoding coiled coil (CC), nucleotide binding site (NB) and leucine-rich repeat (LRR) domain-containing protein. Arabidopsis genome contains 〜200 member of RCY1 paralogs encoding NB-LRR protein. In this project, the function of RCY1 and other NB-LRR genes for the resistance to CMV was analyzed using a serious of RCY1 mutants and NB-LRR custom array. The obtained results suggested that (1)all CC, NB and LRR domains are required for complete resistance to CMV, (2)the resistance to CMV is dependent on the level of RCY1 expression, (3)RCY1 separately regulates development of hypersensitive cell death and restriction of virus spread. Furthermore, global analysis of 〜200 members of NB-LRR gene family in CMV resistance using custom array indicated that expression of five NB-LRR genes : At1g72890,At1g72900,At1g72910,At1g72930,At1g72940, which tandemly located on the chromosome I, was up-regulated in CMV resistance. The CMV-inducible R-like gene cluster was named "CIR1". To further analyze the function of CIR1 for CMV resistance, we transformed CIR1 cDNA under the control of CaMV35S promoter into A. thaliana carrying RCY1. The response of the obtained transformants to CMV will be extensively analyzed. The information obtained from this research will give a new insight to study the role of NB-LRR gene family for CMV resistance and molecular basis of virus resistance in plants.

Research Products

• [Journal Article] Up-regulation of LeATL6 that encodes a fungal elicitor-responsive ubiquitin ligase induces jasmonic acid-dependent proteinase inhibitor gene expression in tomato. (2007)
  • Author(s): Hondo, D. et al.
  • Journal Title: Molecular Plant-Microbe Interactions 20 Pages: 72-81
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of a novel Cys_2/His_2-type zinc-finger protein as a component of a spermine-signaling pathway in tobacco. (2007)
  • Author(s): Mitsuya, Y. et al.
  • Journal Title: Journal of Plant Physiology (印刷中)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Up-regulation of LeA7L6 that encodes a fungal elicitor-responsive ubiquitin ligase induces jasmonic acid-dependent proteinase inhibitor gene expression in tomato. (2007)
  • Author(s): Hondo, D., Hase, S., Kanayama, Y., Yoshikawa, N., Takenaka, S., Takahashi, H.
  • Journal Title: Molecular Plant-Microbe Interactions 20 Pages: 72-81
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of a novel CYs_2/His_2-type zinc-finger protein as a component of a spemine-signaling pathway in tobacco. (2007)
  • Author(s): Mitsuya, Y., Takahashi, Y., Uehara, Y., Barberich, T., Miyazaki, A., Takahashi, H., Kusano, T.
  • Journal Title: Journal of Plant Physiology (m press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Up-regulation of LeATL6 that encodes a fungal elicitor-responsive ubiquitin ligase induces jasmonic acid-dependent proteinase inhibitor gene expression in tomato. (2007)
  • Author(s): Hondo, D., et al.
  • Journal Title: Molecular Plant-Microbe Interactions 20 Pages: 72-81
• [Journal Article] Identification of a novel Cys_2/His_2-type zinc-finger protein as a component of a spermine-signaling pathway in tobacco. (2007)
  • Author(s): Mitsuya, Y., et al.
  • Journal Title: Journal of Plant Physiology (印刷中)
• [Journal Article] Single amino acid alterations in Arabidopsis thaliana RCY1 compromise resistance to Cucumber mosaic virus, but differentially suppress hypersensitive response-like cell death. (2006)
  • Author(s): Sekine, K.-T.et al.
  • Journal Title: Plant Molecular Biology 62 Pages: 669-682
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Beta-cyanoalanine synthase as a molecular marker induced resistance by fungal glycoprotein elicitor and commercial plant activators. (2006)
  • Author(s): Takahashi, H. et al.
  • Journal Title: Phytopathology 96 Pages: 908-916
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Induction of transient ethylene and reduction in severity of tomato bacterial wilt by Pythium oligandrum. (2006)
  • Author(s): Hase, S. et al.
  • Journal Title: Plant Pathology 55 Pages: 537-543
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Novel elicitin-like proteins isolated from the cell wall of the biocontrol agent Pythium oligandrum induce defence-related genes in sugar beet. (2006)
  • Author(s): Takenaka, S. et al.
  • Journal Title: Molecular Plant Pathology 7 Pages: 325-339
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Single amino acid alterations in Arabidosis thaliana RCY1 compromise resistance to Cucumber mosaic virus, but differentially suppress hypersensitive response-like cell death. (2006)
  • Author(s): Sekine, K.-T., Ishihara, T., Hase, S., Kusano, T., Shah, J., Takahashi, H.
  • Journal Title: Plant Molecular Biology 62 Pages: 669-682
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Beta-cyanoalanine synthase as a molecular marker induced resistance by fungal glycopotein elicitor and commercial plant activators. (2006)
  • Author(s): Takahashi, H., Ishihara, T., Hase, S., Chiba, A., Nakaho, K., Arie, T., Teraoka, T., Iwata, M., Tugane, T., Shibata, D., Takenaka, S.
  • Journal Title: Phytopathology 96 Pages: 908-916
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Induction of transient ethylene and reduction in severity of tomato bacterial wilt by Pythium oligandrum. (2006)
  • Author(s): Hase, S., Shimizu, A., Nakaho, K., Takenaka, S., Takahashi, H.
  • Journal Title: Plant Pathology 55 Pages: 537-543
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Novel elicitin-like proteins isolated from the cell wall of the biocontrol agent Pythium oligandrum induce defence-related gars in sugar beet (2006)
  • Author(s): Takenaka, S., Nakamura, Y., Ono, T., Sekiguchi, H., Masunaka, A., Takahashi, H.
  • Journal Title: Molecular Plant Pathology 7 Pages: 325-339
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Single amino acid alterations in Arabidopsis thaliana RCY1 compromise resistance to Cucumber mosaic virus, but differentially suppress hypersensitive response-like cell death. (2006)
  • Author(s): Sekine, K.-T., et al.
  • Journal Title: Plant Molecular Biology 62 Pages: 669-682
• [Journal Article] Novel elicitin-like proteins isolated from the cell wall of the biocontrol agent Pythium oligandrum induce defence-related genes in sugar beet. (2006)
  • Author(s): Takenaka, S., et al.
  • Journal Title: Molecular Plant Pathology 7 Pages: 325-339
• [Journal Article] Beta-cyanoalanine synthase as a molecular marker induced resistance by fungal glycoprotein elicitor and commercial plant activators. (2006)
  • Author(s): Takahashi, H., et al.
  • Journal Title: Phytopathology 96 Pages: 908-916
• [Journal Article] Catalog of Micro-Tom tomato responses to common fungal, bacterial and viral pathogens. (2005)
  • Author(s): Takahashi, H. et al.
  • Journal Title: Journal of General Plant Pathology 71 Pages: 8-22
  • NAID: 10014471856
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Tobacco ZFT1, a transcriptional repressor with a Cys_2/His_2 type zinc finger motif that functions in spermine-signaling pathway. (2005)
  • Author(s): Uehara, Y. et al.
  • Journal Title: Plant Molecular Biology 59 Pages: 433-446
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of the cis-acting elements in Arabidopsis thaliana NHL10 promoter responsible for leaf senescence, the hypersensitive response against Cucumber mosaic virus infection, and spermine treatment. (2005)
  • Author(s): Zheng, M.S.et al.
  • Journal Title: Plant Science 168 Pages: 415-422
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Catalog of Micro-Tom tomato responses to common fungal, bacterial and viral Pathogens. (2005)
  • Author(s): Takahashi, H., Shimizu, A., Arie, T., Rosmalawati, S., Fukushima, S., Kikuchi, M., Hikichi, Y., Kanda, A., Ohnishi, K., Ichinose, Y., Yasuda, C., Kodama, M., Egusa, M., Masuta, C., Sawada, H., Shibata, D., Hori, K., Watanabe, Y.
  • Journal Title: Joumal of General Plant Pathology 71 Pages: 8-22
  • NAID: 10014471856
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Tobacco ZFT1, a transcriptional repressor with a Cys_2/His_2 type zinc finger motif that functions in in spermine-signaling pathway. (2005)
  • Author(s): Uehara, Y., Takahashi, Y., Berberich, T., Miyazaki, A., Takahashi, H., Matsui, K., Ohme-Takagi, M., Saitoh, H., Terauchi, R., Kusano, T.
  • Journal Title: Plant Molecular Biology 59 Pages: 433-446
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of the cis-acting dements in Arabidopsis thaliana NHL10 promoter responsible for leaf senescence, the hypersensitive response against Cucumber mosaic virus infection, and spermine treatment. (2005)
  • Author(s): Zheng, M.S., Takahashi, H., Miyazaki, A., Yamaguchi, K., Kusano, T.
  • Journal Title: Plant Science 168 Pages: 415-422
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Antagonistic interactions between the SA- and JA- signaling pathways in Arabidopsis modulate expression of defense genes and gene-for-gene resistance to Cucumber mosaic virus. (2004)
  • Author(s): Takahashi, H. et al.
  • Journal Title: Plant Cell Physiology 45 Pages: 803-809
  • NAID: 10013129021
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Enhanced resistance to Cucumber mosaic virus in the Arabidopsis thalianassi2 mutant is mediated via an SA-independent mechanism. (2004)
  • Author(s): Sekine, K.-T.et al.
  • Journal Title: Molecular Plant-Microbe Interaction 17 Pages: 623-632
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Comparative analysis of expressed sequence tags in resistant and susceptible ecotypes of Arabidopsis thaliana infected with Cucumber mosaic virus. (2004)
  • Author(s): Ishihara, T. et al.
  • Journal Title: Plant Cell Physiology 45 Pages: 470-480
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Up-regulation of Arabidopsis thaliana NHL10 in the hypersensitive response to Cucumber mosaic virus infection and in senescing leaves is controlled by signaling pathways that differ in salicylate involvement. (2004)
  • Author(s): Zheng, M.S.et al.
  • Journal Title: Planta 218 Pages: 740-750
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Antagonistic interactions between the SA-and IA-signaling pathways in Arabidopsis modulate expression of defense genes and gene-for-gene resistance to Cucumbermasaicvirus (2004)
  • Author(s): Takahashi, H, Kanayama, Y., Zheng, M.S., Kusano, T., Hase, S., Ikegami, M., Shah, S.
  • Journal Title: Plant Cell Physiology 45 Pages: 803-809
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Enhanced resistance to Cucumber mosaic virus in the Anabidopsis thaliana ssi2 mutant is mediated via an SA-independent mechanism. (2004)
  • Author(s): Sekine, K.-T., Nandi, A., Ishihara, T., Hase, S., Ikegami, M., Shah, S., Takahashi, H.
  • Journal Title: Molecular Plant-Microbe Interaction 17 Pages: 623-632
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Comparative analysis of expressed sequence tags in resistant and susceptible ecotypes of Arabidopsis thaliana infected with Cucumber mosaic virus. (2004)
  • Author(s): Ishihara, T., Sakurai, N., Sekine, K.-T., Hase, S., Ikegami, M., Shibata, D., Takahashi, H.
  • Journal Title: PlantCell Physiology 45 Pages: 470-480
  • NAID: 10012852556
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Up-regulation of Arabidopsis thaliana NHL10 in the hypersensitive response to Cucumber mosaic virus infection and in senescing leaves is controlled by signaling pathways that differ in salicylate involvement (2004)
  • Author(s): Zheng, M.S., Takahashi, H., Miyazaki, A., Hamamoto, H., Shah, J., Yamaguchi, I., Kusano, T.
  • Journal Title: Planta 218 Pages: 740-750
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Antagonistic interactions between the SA- and JA- signaling pathways in Arabidopsis modulate expression defense genes and gene-for-gene resistance to Cucumber mosaic virus. (2004)
  • Author(s): Takahashi et al.
  • Journal Title: Plant Cell Physiology 45 Pages: 803-809
  • NAID: 10013129021
• [Journal Article] The enhanced resistance to Cucumber mosaic virus in the Arabidopsis thaliana stearoyl-ACP desaturase-deficient ssi2 mutant is mediated by an SA-independent, but EDS5-dependent signaling mechanism. (2004)
  • Author(s): Sekine et al.
  • Journal Title: Molecular Plant-Microbe Interaction 17 Pages: 623-632
• [Journal Article] Comparative analysis of expressed sequence tags in resistant and susceptible ecotypes of Arabidopsis thaliana infected with Cucumber mosaic virus. (2004)
  • Author(s): Ishihara et al.
  • Journal Title: Plant Cell Physiology 45 Pages: 470-480
• [Journal Article] Identification of the cis-acting elements in Arabidopsis thaliana NHL10 promoter responsible for leaf senescence, the hypersensitive response against Cucumber mosaic virus infection, and spermine treatment. (2004)
  • Author(s): Zheng et al.
  • Journal Title: Plant Science 168 Pages: 415-422
• [Book] 「ウイルス感染に対する宿主応答」作物保護の新展開-バイオサイエンスのかけはし- (羽柴輝良 監修) (2005)
  • Author(s): 高橋英樹
  • Total Pages: 338
  • Publisher: ソフトサイエンス社
  • Description: 「研究成果報告書概要(和文)」より
• [Book] "Signal transduction pathways for the resistance response to Cucumber mosaic virus." Genomic and Genetic Analysis of Plant Parasitism and Defense. (Eds. S. Tsuyumu, J. Leach, T. Shiraishi and T. Walpert) (2005)
  • Author(s): Takahashi, H. et al.
  • Total Pages: 301
  • Publisher: APS Press
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Response of host plants to virus infection, New insight of Plant Protection (edited by Hashiba, T.)(in Japanese) (2005)
  • Author(s): Takahashi, H.
  • Total Pages: 338
  • Publisher: Softscience-sha
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Signal transduction pathways for the resistance response to Cucumber mosaic virus. Genomic and Genetic Analysis of Plant Parasitism and Defense (Eds.S.Tsuyumu, J.Leach, T.Shiraishi and T.Walpert) (2005)
  • Author(s): Takahashi, H., Sekine, K.-T., Ishihara, T., Hase, S., Ikegami, M., Shah, J.
  • Total Pages: 301
  • Publisher: APS Press, St Paul, Minnesota, USA
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 「ウイルスに対する宿主抵抗性」新版 分子レベルからみた植物の耐病性 植物細胞工学シリーズ19(島本 功・渡辺雄一郎・柘植尚志 監修) (2004)
  • Author(s): 高橋英樹
  • Total Pages: 214
  • Publisher: 秀潤社
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Resistance to virus in host plants, Disease resistance in plants at the molecular level (edited by Shimamoto, K., Watanabe, Y.and Tsuge, T.) Plant Biotechology 19(in Japanese) (2004)
  • Author(s): Takahashi, H.
  • Total Pages: 214
  • Publisher: Shuujyunnsha
  • Description: 「研究成果報告書概要(欧文)」より
• [Patent(Industrial Property Rights)] 植物病原体に対する誘導抵抗性の指標となるマーカー遺伝子の単離 (2005)
  • Inventor(s): 高橋英樹, 竹中重仁
  • Industrial Property Rights Holder: 東北大学・(独)農業・生物系産業技術研究機構
  • Industrial Property Number: 2005-353502
  • Filing Date: 2005-12-07
  • Acquisition Date: 2005-12-26
  • Description: 「研究成果報告書概要(和文)」より