Molecular heterogeneity and physiological functions of cation/H^+ exchanger and proton pumps in vacuolar membranes

• Project/Area Number: 16380068
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Nagoya University
• Principal Investigator: MAESHIMA Masayoshi Nagoya University, Graduate School of Bioagricultural Sciences, Professor, 大学院・生命農学研究科, 教授 (80181577)
• Co-Investigator(Kenkyū-buntansha): NAKANISHI Yoichi Nagoya University, Graduate School of Bioagricultural Sciences, Assistant Professor, 大学院・生命農学研究科, 助手 (60362290)
• Project Period (FY): 2004 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥15,600,000 (Direct Cost: ¥15,600,000); Fiscal Year 2005: ¥9,100,000 (Direct Cost: ¥9,100,000); Fiscal Year 2004: ¥6,500,000 (Direct Cost: ¥6,500,000)
• Keywords: Ca^<2+> / H^+ exchanger / Rice / Ion pool / Vacuolar membrane / Cell specificity / Metal toxicity / Proton pump / ポロトンポンプ / H^+-ピロホスファターゼ / 分子構築 / 遺伝子欠損株 / 機能ドメイン / 結晶構造 / 高次構造

Research Abstract

During the project period for two years, we obtained the following results concerning metal transporters and proton pumps in the vacuolar membranes.
(1) Cation/H^+ exchangers : Rice has five isoforms of cation/H^+ exchanger (CAX). Our study revealed that these five isoforms varied with metal ion selectivity and tissue specificity of gene expression. CAX1a among them is expressed in all tissues examined and is highly expressed in companion and endodermis cells, which has high concentration of Ca^<2+>. We estimate that CAX1a functions to reserve Ca^<2+> into vacuoles and is involved in the Ca^<2+> detoxification.
(2) Vacuolar zinc transporter (MTP1) : Arabidopsis MTP1 has been demonstrated to be located to the vacuolar membrane. The knockout mutant of MTP1 was highly sensitive to high concentrations (more than 0.2 mM) of zinc. Thus, MTP1 might be involved in the detoxification of zinc by transporting ion in the cytoplasm into vacuoles. By heterologous expression of MTP1 in yeast and direct measurement of zinc transport using Zn-65, we demonstrated that MTP1 functions as Zn^<2+>/H^+ exchanger.
(3) Vacuolar proton pump : Vacuolar membrane H+-pyrophosphatase (H^+-PPase) acidifies vacuoles and maintains the pH gradient across the membrane. Our experiments revealed that a knockout mutant of H^+-PPase could not grow normally, suggesting that H+-PPase is one of the essential membrane transport systems in plants. We also determined the membrane topology of H^+-PPase and essential motifs in the enzyme. Our structural model has been accepted as a standard of H^+-PPases in various organisms.

Research Products

• [Journal Article] Expression of the vacuolar Ca^<2+>/H^+ exchanger, OsCAXla, in rice : cell-and age-specificity of expression and enhancement by Ca^<2+>. (2006)
  • Author(s): Kamiya, T.
  • Journal Title: Plant Cell Physiol. 47 Pages: 96-106
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Immunochemical analysis of aquaporin isoforms in Arabidopsis suspension cultured cells. (2006)
  • Author(s): Kobae, Y.
  • Journal Title: Biosci. Biotechnol. Biochem. 70 Pages: 980-987
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Disruption of AtPDR8, a plasma membrane ABC transporter of Arabidopsis, exhibits hypersensitive cell death by pathogen infection. (2006)
  • Author(s): Kobae, Y.
  • Journal Title: Plant Cell Physiol. 47 Pages: 309-318
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Subcellular localization of Strboh proteins and NADPH-dependent O_2^-generating activity in potato tuber tissues. (2006)
  • Author(s): Kobayashi, M.
  • Journal Title: J. Exp. Botany 57 Pages: 1373-1379
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression of the vacuolar Ca^<2+>/H^+ exchanger, OsCAX1a, in rice : cell- and age-specificity of expression and enhancement by Ca^<2+>. (2006)
  • Author(s): Kamiya, T., Akahori, T., Ashikari, M., Maeshima, M.
  • Journal Title: Plant Cell Physiol. 47 Pages: 96-106
  • NAID: 10017158016
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Immunochemical analysis of aquaporin isoforms in Arabidopsis suspension cultured cells. (2006)
  • Author(s): Kobae, Y., Mizutani, M., Segami, S., Maeshima, M.
  • Journal Title: Biosci.Biotechnol.Biochem. 70 Pages: 980-987
  • NAID: 10018532678
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Disruption of AtPDR8, a plasma membrane ABC transporter of Arabidopsis, exhibits hypersensitive cell death by pathogen infection. (2006)
  • Author(s): Kobae, Y., Yoshioka, H., Sekino, T., Nakanishi, Y., Martinoia, E., Maeshima, M.
  • Journal Title: Plant Cell Physiol. 47 Pages: 309-318
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Subcellular localization of Strboh proteins and NADPH-dependent O_2^- generating activity in potato tuber tissues. (2006)
  • Author(s): Kobayashi, M., Yoshioka, H., Kawakita, K., Maeshima, M., Doke, N.
  • Journal Title: J.Exp.Bot. 57 Pages: 1373-1379
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of the vacuolar Ca^<2+>/H^+ exchanger, OsCAX1a, in rice : cell- and age-specificity of expression and enhancement by Ca^<2+>. (2006)
  • Author(s): Kamiya, T.
  • Journal Title: Plant Cell Physiol. 47 Pages: 96-106
• [Journal Article] Immunochemical analysis of aquaporin isoforms in Arabidopsis suspension cultured cells. (2006)
  • Author(s): Kobae, Y.
  • Journal Title: Biosci.Biotechnol.Biochem. Apr.issue (In press)
• [Journal Article] Subcellular localization of Strboh proteins and NADPH-dependent O_2^- generating activity in potato tuber tissues. (2006)
  • Author(s): Kobayashi, M.
  • Journal Title: J.Exp.Botany 57 Pages: 1373-1379
• [Journal Article] The involvement of tonoplast proton pumps and Na^+(K^+)/H^+ exchangers in the change of petal color during flower-opening of morning glory, Ipomoea tricolor cv. Heavenly Blue. (2005)
  • Author(s): Yoshida, K.
  • Journal Title: Plant Cell Physiol. 46 Pages: 407-415
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A new and simple technique for the isolation of plasma membrane lipids from root-tips. (2005)
  • Author(s): Wagatsuma, T.
  • Journal Title: Soil Sci. Plant Nutr. 51 Pages: 135-139
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Oligomerization of the H^+-pyrophosphatase and its structural and functional consequences. (2005)
  • Author(s): Mimura, H.
  • Journal Title: Biochim. Biophys. Acta 1708 Pages: 393-403
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Disulfide bond formation in the H^+-pyrophosphatase of Streptomyces coelicolor and its implication in redox control and structure. (2005)
  • Author(s): Mimura, H.
  • Journal Title: FEBS Letters 579 Pages: 3625-3631
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Enzymatic and molecular properties of H^+-pyrophosphatase of Streptomyces coelicolor expressed in Escherichia coli. (2005)
  • Author(s): Hirono, M.
  • Journal Title: J. Biochem. 138 Pages: 183-191
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The distribution of aquaporins subtypes (PIP1, PIP2 and γ-TIP) is tissue dependent in soybean (Glycine max) root nodules. (2005)
  • Author(s): Fleurat-Lessard, P.
  • Journal Title: Annals Bot. 96 Pages: 457-460
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Characterization of vacuolar membrane proteins changed in rice treated with gibberellin. (2005)
  • Author(s): Konishi, H.
  • Journal Title: J. Proteome Res. 4 Pages: 1755-1780
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of 33 rice aquaporin genes and analysis of their expression and function. (2005)
  • Author(s): Sakurai, J.
  • Journal Title: Plant Cell Physiol. 46 Pages: 1568-1577
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression profile of rice cation/H^+ exchanger family and heterologous expression in yeast. (2005)
  • Author(s): Kamiya, T.
  • Journal Title: Plant Cell Physiol. 46 Pages: 1735-1740
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Three SIP aquaporins of Arabidopsis are localized in the ER membrane and expressed in a tissue and cell-specific manner. (2005)
  • Author(s): Ishikawa, F.
  • Journal Title: FEBS Letters 579 Pages: 5814-5820
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The involvement of tonoplast proton pumps and Na^+(K^+)/H^+ exchangers in the change of petal color during flower-opening of morning glory, Ipomoea tricolor cv. Heavenly Blue. (2005)
  • Author(s): Yoshida, K., Kawachi, M., Mori, M., Maeshima, M., Kondo, M., Nishimura, M., Kondo, T.
  • Journal Title: Plant Cell Physiol. 46 Pages: 407-415
  • NAID: 10015598502
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A new and simple technique for the isolation of plasma membrane lipids from root-tips. (2005)
  • Author(s): Wagatsuma, T., Uemura, M., Mitsuhashi, W., Maeshima, M., Ishikawa, S., Kawamura, T., Murayama, T., Shiono, Y., Khan, M.S.H., Tawaraya, K.
  • Journal Title: Soil Sci.Plant Nutr. 51 Pages: 135-139
  • NAID: 110004704730
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Oligomerization of the H^+-pyrophosphatase and its structural and functional consequences. (2005)
  • Author(s): Mimura, H., Nakanishi, Y., Maeshima, M.
  • Journal Title: Biochim.Biophys.Acta-Bioenergetics 1708 Pages: 393-403
  • NAID: 120000979445
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Disulfide bond formation in the H^+-pyrophosphatase of Streptomyces coelicolor and its implication in redox control and structure. (2005)
  • Author(s): Mimura, H., Nakanishi, Y., Maeshima, M.
  • Journal Title: FEBS Letters 579 Pages: 3625-3631
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Enzymatic and molecular properties of H^+-pyrophosphatase of Streptomyces coelicolor expressed in Escherichia coli. (2005)
  • Author(s): Hirono, M., Mimura, H., Nakanishi, Y., Maeshima, M.
  • Journal Title: J.Biochem. 138 Pages: 183-191
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The distribution of aquaporins subtypes (PIP1, PIP2 and γ-TIP) is tissue dependent in soybean (Glycine max) root nodules. (2005)
  • Author(s): Fleurat-Lessard, P., Michonneau, P., Maeshima, M., Drevon, J.J., Serraj, R.
  • Journal Title: Annals of Botany 96 Pages: 457-460
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of vacuolar membrane proteins changed in rice treated with gibberellin. (2005)
  • Author(s): Konishi, H., Maeshima, M., Komatsu, S.
  • Journal Title: J.Proteome Res. 4 Pages: 1755-1780
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of 33 rice aquaporin genes and analysis of their expression and function. (2005)
  • Author(s): Sakurai, J., Ishikawa, F., Yamaguchi, T., Uemura, M., Maeshima, M.
  • Journal Title: Plant Cell Physiol. 46 Pages: 1568-1577
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression profile of rice cation/H^+ exchanger family and heterologous expression in yeast. (2005)
  • Author(s): Kamiya, T., Akahori, T., Maeshima, M.
  • Journal Title: Plant Cell Physiol. 46 Pages: 1735-1740
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Three SIP aquaporins of Arabidopsis are localized in the ER membrane and expressed in a tissue- and cell-specific manner. (2005)
  • Author(s): Ishikawa, F., Suga, S., Uemura, T., Sato, M.H., Maeshima, M.
  • Journal Title: FEBS Lett. 579 Pages: 5814-5820
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Japan Scientific Societies Press, (2005)
  • Author(s): Maeshima, M.
  • Journal Title: Biochemistry and Molecular Biology of Plants. (Japanese edition).
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Kuba Pro. (2005)
  • Author(s): Maeshima, M.
  • Journal Title: Mechanisms in Fresh Bodies : Function of Aquaporins and Its Relation with Diseases. Pages: 20-32
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The involvement of tonoplast proton pumps and Na^+(K^+)/H^+ exchangers in the change of petal color during flower-opening of morning glory, Ipomoea tricolor cv.Heavenly Blue. (2005)
  • Author(s): Yoshida, K.
  • Journal Title: Plant Cell Physiol. 46 Pages: 407-415
• [Journal Article] A new and simple technique for the isolation of plasma membrane lipids from root-tips. (2005)
  • Author(s): Wagatsuma, T.
  • Journal Title: Soil Sci.Plant Nutr. 51 Pages: 135-139
• [Journal Article] Oligomerization of the H^+-pyrophosphatase and its structural and functional consequences. (2005)
  • Author(s): Mimura, H.
  • Journal Title: Biochim.Biophys.Acta 1708 Pages: 393-403
• [Journal Article] Enzymatic and molecular properties of H^+-pyrophosphatase of Streptomyces coelicolor expressed in Escherichia coli. (2005)
  • Author(s): Hirono, M.
  • Journal Title: J.Biochem. 138 Pages: 183-191
• [Journal Article] The distribution of aquaporins subtypes (PIP1,PIP2 and γ-TIP) is tissue dependent in soybean (Glycine max) root nodules. (2005)
  • Author(s): Fleurat-Lessard, P.
  • Journal Title: Annlas Bot. 96 Pages: 457-460
• [Journal Article] Characterization of vacuolar membrane proteins changed in rice treated with gibberellin. (2005)
  • Author(s): Konishi, H.
  • Journal Title: J.Proteome Res. 4 Pages: 1755-1780
• [Journal Article] The involvement of tonoplast proton pumps and Na^+(K^+)/H^+ exchangers in the change of petal color during flower-opening of morning glory, Ipomoea tricolor cv.Heavenly Blue. (2005)
  • Author(s): Yoshida, K.
  • Journal Title: Plant and Cell Physiology 46 Pages: 407-415
• [Journal Article] Residues in internal repeats of the rice cation/H^+ exchanger are involved in the transport and selection of cations. (2004)
  • Author(s): Kamiya, T.
  • Journal Title: J. Biol. Chem. 279 Pages: 812-819
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effect of salt and osmotic stresses on the expression of genes fro the vacuolar H^+-pyrophosphatase, H^+-ATPase subunit A and Na^+/H^+ antiporter from barley. (2004)
  • Author(s): Fukuda, A.
  • Journal Title: J. Exp. Botany 55 Pages: 585-594
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Arabidopsis immunophilin-like TWD1 functionally interacts with vacuolar ABC transporters. (2004)
  • Author(s): Geisler, M.
  • Journal Title: Mol. Biol. Cell 15 Pages: 3393-3405
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Isolation of intact vacuoles and proteomic anlaysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana. (2004)
  • Author(s): Shimaoka, T.
  • Journal Title: Plant Cell Physiol. 45 Pages: 672-683
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Contribution of the plasma membrane and central vacole in the formation of autolysosomes in tobacco cultured cells. (2004)
  • Author(s): Yano, K.
  • Journal Title: Plant Cell Physiol. 45 Pages: 951-957
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Water channel activity of radish plasma membrane aquaporins heterologously expressed in yeast and their modification by sitedirected mutagenesis. (2004)
  • Author(s): Suga, S.
  • Journal Title: Plant Cell Physiol. 45 Pages: 823-830
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Membrane topology of the H^+-pyrophosphatase of Streptomyces coelicolor determined by cysteine-scanning mutagenesis. (2004)
  • Author(s): Mimura, H.
  • Journal Title: J. Biol. Chem. 279 Pages: 35106-35112
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Functional identification of the glyceroaquaporin of Chlamydomonas reinhardtii CrMIP1. (2004)
  • Author(s): Anderca, M.
  • Journal Title: Plant Cell Physiol. 45 Pages: 1313-1319
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Characterization of fructose-bisphosphate aldolase regulated by gibberellin in roots of rice seedling. (2004)
  • Author(s): Konishi, H.
  • Journal Title: Plant Mol.Biol. 56 Pages: 839-848
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. (2004)
  • Author(s): Kobae, Y.
  • Journal Title: Plant Cell Physiol. 45 Pages: 1749-1758
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Residues in internal repeats of the rice cation/H^+ exchanger are involved in the transport and selection of cations. (2004)
  • Author(s): Kamiya, T., Maeshima, M.
  • Journal Title: J.Biol.Chem. 279 Pages: 812-819
  • NAID: 120000979762
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effect of salt and osmotic stresses on the expression of genes for the vacuolar H^+-pyrophosphatase, H^+-ATPase subunit A and Na^+/H^+ antiporter from barley. (2004)
  • Author(s): Fukuda, A., Chiba, K., Maeda, M., Nakamura, A., Maeshima, M., Tanaka, Y.
  • Journal Title: J.Exp.Botany 55 Pages: 585-594
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Arabidopsis immunophilin-like TWD1 functionally interacts with vacuolar ABC transporters. (2004)
  • Author(s): Geisler, M., Girin, M., Brnadt, S., Vincenzetti, V., Plaza, S., Paris, N., Kobae, Y., Maeshima, M., Billion, K., Kolukisaoglu, U.H., Schulz, B., Martinoia, E.
  • Journal Title: Mol.Biol.Cell. 15 Pages: 3393-3405
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana. (2004)
  • Author(s): Shimaoka, T., Ohnishi, M., Sazuka, T., Mitsuhashi, N., Hara-Nishimura, I., Shimazaki, K., Maeshima, M., Yokota, A., Tomizawa, K., Mimura, T.
  • Journal Title: Plant Cell Physiol. 45 Pages: 672-683
  • NAID: 10013128417
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Contribution of the plasma membrane and central vacuole in the formation of autolysosomes in tobacco cultured cells. (2004)
  • Author(s): Yano, K., Matsui, S., Tsuchiya, T., Maeshima, M., Kutsuna, N., Hasezawa, S., Moriyasu, Y.
  • Journal Title: Plant Cell Physiol. 45 Pages: 951-957
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Water channel activity of radish plasma membrane aquaporins heterologously expressed in yeast and their modification by site-directed mutagenesis. (2004)
  • Author(s): Suga, S., Maeshima, M.
  • Journal Title: Plant Cell Physiol. 45 Pages: 823-830
  • NAID: 10013345141
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Membrane topology of the H^+-pyrophosphatase of Streptomyces coelicolor determined by cysteine-scanning mutagenesis. (2004)
  • Author(s): Mimura, H., Nakanishi, Y., Hirono, M., Maeshima, M.
  • Journal Title: J.Biol.Chem. 279 Pages: 35106-35112
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functional idientification of the glycerolaquaporin of Chlamydomonas reinhardtii CrMIP1. (2004)
  • Author(s): Anderca, M.I., Suga, S., Furuich, T., Shimogawara, K., Maeshima, M., Muto S.
  • Journal Title: Plant Cell Physiol. 45 Pages: 1313-1319
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of fructose-bisphosphate aldolase regulated by gibberellin in roots of rice seedling. (2004)
  • Author(s): Konishi, H., Yamane, H., Maeshima, M., Komatsu, S.
  • Journal Title: Plant Mol.Biol. 56 Pages: 839-848
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. (2004)
  • Author(s): Kobae, Y., Uemura, T., Sato, M.H., Ohnishi, M., Mimura, T., Maeshima, M.
  • Journal Title: Plant Cell Physiol. 45 Pages: 1749-1758
  • NAID: 10014214266
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Residues in internal repeats of the rice cation/H^+ exchanger are involved in the transport and selection of cations. (2004)
  • Author(s): Kamiya, T.
  • Journal Title: Journal of Biological Chemistry 279 Pages: 812-819
• [Journal Article] Effect of salt and osmotic stresses on the expression of genes for the vacuolar H^+-pyrophosphatase, H^+-ATPase subunit A and Na^+/H^+ antiporter from barley. (2004)
  • Author(s): Fukuda, A.
  • Journal Title: Journal of Experimental Botany 55 Pages: 585-594
• [Journal Article] Arabidopsis immunophilin-kike TWD1 modulates vacuolar ABC transporter activity by means of protein-protein interaction. (2004)
  • Author(s): Geisler, M.
  • Journal Title: Molecular Biology of the Cell 15 Pages: 3393-3405
• [Journal Article] Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana. (2004)
  • Author(s): Shimaoka, T.
  • Journal Title: Plant and Cell Physiology 45 Pages: 672-683
• [Journal Article] Contribution of the plasma membrane and central vacuole in the formation of autolysosomes in tobacco cultured cells. (2004)
  • Author(s): Yano, K.
  • Journal Title: Plant and Cell Physiology 45 Pages: 951-957
• [Journal Article] Water channel activity of radish plasma membrane aquaporins heterologously expressed in yeast and their modification by sitedirected mutagenesis. (2004)
  • Author(s): Suga, S.
  • Journal Title: Plant and Cell Physiology 45 Pages: 823-830
• [Journal Article] Membrane topology of the H^+-pyrophosphatase of Streptomyces coelicolor determined by cysteine-scanning mutagenesis. (2004)
  • Author(s): Mimura, H.
  • Journal Title: Journal of Biological Chemistry 279 Pages: 35106-35112
• [Journal Article] Functional idientification of the glycerolaquaporin of Chlamydomonas reinhardtii CrMIP1. (2004)
  • Author(s): Anderca, M.I.
  • Journal Title: Plant and Cell Physiology 45 Pages: 1313-1319
• [Journal Article] Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. (2004)
  • Author(s): Kobae, Y.
  • Journal Title: Plant and Cell Physiology 45 Pages: 1749-1758
• [Journal Article] Characterization of fructose-bisphosphate aldolase regulated by gibberellin in roots of rice seedling. (2004)
  • Author(s): Konishi, H.
  • Journal Title: Plant Molecular Biology 56 Pages: 839-848
• [Journal Article] ノーベル化学賞のスポットライトをあびたアクアポリン (2004)
  • Author(s): 前島正義
  • Journal Title: 化学と生物 42 Pages: 41-42
  • NAID: 10012135532
• [Book] 植物の生化学・分子生物学(分担翻訳および監訳) (2005)
  • Author(s): 前島正義
  • Total Pages: 1234
  • Publisher: 学会出版センター
  • Description: 「研究成果報告書概要(和文)」より
• [Book] みずみずしい体のしくみ : 水の通り道「アクアポリン」の働きと病気 (2005)
  • Author(s): 前島正義
  • Total Pages: 183
  • Publisher: クバプロ
  • Description: 「研究成果報告書概要(和文)」より
• [Book] みずみずしい体のしくみ:水の通り道「アクアポリン」の働きと病気 (2005)
  • Author(s): 前島正義
  • Total Pages: 183
  • Publisher: クバプロ
• [Book] みずみずしい体のしくみ-水の通り道:アクアポリンの働きと病気(分担:みずみずしい植物とアクアポリン) (2005)
  • Author(s): 前島正義(分担執筆)
  • Publisher: グバプロ(印刷中)