Molecular heterogeneity and physiological functions of cation/H^+ exchanger and proton pumps in vacuolar membranes
| Project/Area Number |
16380068
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
MAESHIMA Masayoshi Nagoya University, Graduate School of Bioagricultural Sciences, Professor, 大学院・生命農学研究科, 教授 (80181577)
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| Co-Investigator(Kenkyū-buntansha) |
NAKANISHI Yoichi Nagoya University, Graduate School of Bioagricultural Sciences, Assistant Professor, 大学院・生命農学研究科, 助手 (60362290)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥15,600,000 (Direct Cost: ¥15,600,000)
Fiscal Year 2005: ¥9,100,000 (Direct Cost: ¥9,100,000)
Fiscal Year 2004: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | Ca^<2+> / H^+ exchanger / Rice / Ion pool / Vacuolar membrane / Cell specificity / Metal toxicity / Proton pump / ポロトンポンプ / H^+-ピロホスファターゼ / 分子構築 / 遺伝子欠損株 / 機能ドメイン / 結晶構造 / 高次構造 |
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| Research Abstract |
During the project period for two years, we obtained the following results concerning metal transporters and proton pumps in the vacuolar membranes.
(1) Cation/H^+ exchangers : Rice has five isoforms of cation/H^+ exchanger (CAX). Our study revealed that these five isoforms varied with metal ion selectivity and tissue specificity of gene expression. CAX1a among them is expressed in all tissues examined and is highly expressed in companion and endodermis cells, which has high concentration of Ca^<2+>. We estimate that CAX1a functions to reserve Ca^<2+> into vacuoles and is involved in the Ca^<2+> detoxification.
(2) Vacuolar zinc transporter (MTP1) : Arabidopsis MTP1 has been demonstrated to be located to the vacuolar membrane. The knockout mutant of MTP1 was highly sensitive to high concentrations (more than 0.2 mM) of zinc. Thus, MTP1 might be involved in the detoxification of zinc by transporting ion in the cytoplasm into vacuoles. By heterologous expression of MTP1 in yeast and direct measurement of zinc transport using Zn-65, we demonstrated that MTP1 functions as Zn^<2+>/H^+ exchanger.
(3) Vacuolar proton pump : Vacuolar membrane H+-pyrophosphatase (H^+-PPase) acidifies vacuoles and maintains the pH gradient across the membrane. Our experiments revealed that a knockout mutant of H^+-PPase could not grow normally, suggesting that H+-PPase is one of the essential membrane transport systems in plants. We also determined the membrane topology of H^+-PPase and essential motifs in the enzyme. Our structural model has been accepted as a standard of H^+-PPases in various organisms.
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Report
(3 results)
Research Products
(75 results)
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[Journal Article] Disruption of AtPDR8, a plasma membrane ABC transporter of Arabidopsis, exhibits hypersensitive cell death by pathogen infection.2006
Author(s)
Kobae, Y., Yoshioka, H., Sekino, T., Nakanishi, Y., Martinoia, E., Maeshima, M.
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Journal Title
Plant Cell Physiol. 47
Pages: 309-318
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The involvement of tonoplast proton pumps and Na^+(K^+)/H^+ exchangers in the change of petal color during flower-opening of morning glory, Ipomoea tricolor cv. Heavenly Blue.2005
Author(s)
Yoshida, K., Kawachi, M., Mori, M., Maeshima, M., Kondo, M., Nishimura, M., Kondo, T.
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Journal Title
Plant Cell Physiol. 46
Pages: 407-415
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A new and simple technique for the isolation of plasma membrane lipids from root-tips.2005
Author(s)
Wagatsuma, T., Uemura, M., Mitsuhashi, W., Maeshima, M., Ishikawa, S., Kawamura, T., Murayama, T., Shiono, Y., Khan, M.S.H., Tawaraya, K.
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Journal Title
Soil Sci.Plant Nutr. 51
Pages: 135-139
NAID
Description
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[Journal Article] The distribution of aquaporins subtypes (PIP1, PIP2 and γ-TIP) is tissue dependent in soybean (Glycine max) root nodules.2005
Author(s)
Fleurat-Lessard, P., Michonneau, P., Maeshima, M., Drevon, J.J., Serraj, R.
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Journal Title
Annals of Botany 96
Pages: 457-460
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Kuba Pro.2005
Author(s)
Maeshima, M.
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Journal Title
Mechanisms in Fresh Bodies : Function of Aquaporins and Its Relation with Diseases.
Pages: 20-32
Description
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[Journal Article] Effect of salt and osmotic stresses on the expression of genes for the vacuolar H^+-pyrophosphatase, H^+-ATPase subunit A and Na^+/H^+ antiporter from barley.2004
Author(s)
Fukuda, A., Chiba, K., Maeda, M., Nakamura, A., Maeshima, M., Tanaka, Y.
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Journal Title
J.Exp.Botany 55
Pages: 585-594
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Arabidopsis immunophilin-like TWD1 functionally interacts with vacuolar ABC transporters.2004
Author(s)
Geisler, M., Girin, M., Brnadt, S., Vincenzetti, V., Plaza, S., Paris, N., Kobae, Y., Maeshima, M., Billion, K., Kolukisaoglu, U.H., Schulz, B., Martinoia, E.
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Journal Title
Mol.Biol.Cell. 15
Pages: 3393-3405
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana.2004
Author(s)
Shimaoka, T., Ohnishi, M., Sazuka, T., Mitsuhashi, N., Hara-Nishimura, I., Shimazaki, K., Maeshima, M., Yokota, A., Tomizawa, K., Mimura, T.
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Journal Title
Plant Cell Physiol. 45
Pages: 672-683
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Contribution of the plasma membrane and central vacuole in the formation of autolysosomes in tobacco cultured cells.2004
Author(s)
Yano, K., Matsui, S., Tsuchiya, T., Maeshima, M., Kutsuna, N., Hasezawa, S., Moriyasu, Y.
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Journal Title
Plant Cell Physiol. 45
Pages: 951-957
Description
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[Journal Article] Functional idientification of the glycerolaquaporin of Chlamydomonas reinhardtii CrMIP1.2004
Author(s)
Anderca, M.I., Suga, S., Furuich, T., Shimogawara, K., Maeshima, M., Muto S.
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Journal Title
Plant Cell Physiol. 45
Pages: 1313-1319
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis.2004
Author(s)
Kobae, Y., Uemura, T., Sato, M.H., Ohnishi, M., Mimura, T., Maeshima, M.
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Journal Title
Plant Cell Physiol. 45
Pages: 1749-1758
NAID
Description
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