Cellular and molecular mechanisms for suppression of allergic response by dietary indigestible oligosaccharides
| Project/Area Number |
16380083
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Hokkaido University |
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Principal Investigator |
SONOYAMA Kei Hokkaido Univ., Research Faculty of Agr., Asso.Prof., 大学院農学研究院, 助教授 (90241364)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUSHIMA Michihiro Obihiro Univ.of Agr.and Vet.Med., Prof., 畜産学部, 教授 (20231558)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥15,600,000 (Direct Cost: ¥15,600,000)
Fiscal Year 2006: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2005: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2004: ¥6,700,000 (Direct Cost: ¥6,700,000)
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| Keywords | oligosaccharide / allergy / chemotaxis / delayed-type hypersensitivity / intestinal flora / mouse / NKT cells / プレバイオティクス / 接触皮膚炎 / 腸内細菌 / ラット |
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| Research Abstract |
We aimed to clarify cellular and molecular mechanisms for suppression of allergic response by α-galactooligosaccharides (α-GOS).
1. Dietary α-GOS did not reduce Con A-induced hepatitis and endotoxin shock in mice. IFN-γ production by mouse splenocytes ex vivo was stimulated by LPS, antigen, and endogenouse and exogenous ligands for NKT cells. Because the IFN-γ production was inhibited by anti-CD1d antibody, CD1d-restricted activation of NKT cells is probably responsible for the IFN-γ production. However, supplementation of γ-GOS did not affect the IFN-γ production. These findings suggest that α-GOS does not influence the activation of NKT cells.
2. Dietary α-GOS reduced the infiltration of peritoneal cells after intraperitoneal challenge of antigen in primed mice. Chemotaxis of peritoneal exudate cells and peripheral leukocytes to peritoneal lavage fluid in vitro was also reduced by dietary α-GOS. However, supplementation of α-GOS did not affect the chemotaxis. It is therefore suggested that dietary α-GOS reduces the expression of some chemokines.
3. Dietary non-digestible oligosaccharides reduced hapten-induced contact hypersensitivity in mice. Because the suppressive effect was correlated with the number of cecal bifidobacteria, prebiotics may reduce type IV allergic reactions such as contact hypersensitivity.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Oral administration of freeze-dried kefir reduces intestinal permeation of and oral sensitization to ovalbumin in mice.2005
Author(s)
Umeda, C., Sonoyama, K., Yamaguchi, N., Saito, R., Akashi, K., Motoshima, H., Kawabata, J.
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Journal Title
Bioscience, Biotechnology and Biochemistry 69
Pages: 249-251
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Allergic airway eosinophilia is suppressed in ovalbumin- sensitized Brown Norway rats fed raffinose and α-linked galactooligosaccharide.2005
Author(s)
Sonoyama, K., Watanabe, H., Watanabe, J., Yamaguchi, N., Yamashita, A., Hashimoto, H., Kikuchi, E., Fujita, K., Okada, M., Mori, S., Kitahata, S., Kawabata, J.
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Journal Title
The Journal of Nutrition 135
Pages: 538-543
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Enzymatic synthesis of α-linked galactooligosaccharide (α-GOS) and its functions.2004
Author(s)
Hashimoto, H., Yamashita, A., Fujita, K., Okada, M., Mori, S., Sonoyama, K., Kitahata, S.
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Journal Title
Journal of Applied Glycoscience 51
Pages: 169-176
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Reduction of allergic airway eosinophilia by dietary raffinose in Brown Norway rats.2004
Author(s)
Watanabe, H., Sonoyama, K., Watanabe, J., Yamaguchi, N., Kikuchi, H., Nagura, T., Aritsuka, T., Fukumoto, K., Kasai, T.
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Journal Title
British Journal of Nutrition 92
Pages: 247-255
Description
「研究成果報告書概要(欧文)」より
Related Report
