Investigation of the roles of arachidonic acid metabolite at the process of placenta discharge at delivery of cattle and development of new delivery induction method
| Project/Area Number |
16380182
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | National Agricultural Research Organization |
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Principal Investigator |
KAMADA Hachiro National Agricultural Research Organization, Molecular Nutrition Research Team, Senior Researcher (70360443)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Masato National Agriculture Research Center for Hokkaido region, Research Team for Dairy Production using Regional Feed Resources, Senior Researcher (50450325)
MURAI Masaru National Agriculture Research Center for Hokkaido region, Research Team for Dairy Production using Regional Feed Resources, Team Reader (00414748)
UEDA Yasuko National Agriculture Research Center for Hokkaido region, Intensive Grazing Research Team, Chief Researcher (30370604)
OHSHITA Tomoko National Agriculture Research Center for Hokkaido region, Research Team for Dairy Production using Regional Feed Resource, Chief Researcher (40391479)
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| Project Period (FY) |
2004 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,360,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥960,000)
Fiscal Year 2007: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2006: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 2004: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | Placenta separation / Oxoarachidonic acid / Matrix metalloproteinase / Lipoxygenase / 胎盤由来繊維芽細胞 / アラキドン酸誘導体 / アラキドン酸 |
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| Research Abstract |
As a time of delivery can not be correctly expected, the attendance in the midnight delivery is unavoidable and hard labor for stock raising farmers. The delivery-control technique to alleviate mental and physical strain on the manager are required. The fetal delivery serving as the first stage of delivery is well understood; however, researches are not in progress regarding the release of unneeded placenta serving as the second stage, and the mechanism which induces the placental release has not been known yet. In this study, identification of signal for placenta release and its practical application for delivery induction method were investigated. It is known that a kind of matrix metalloproteinases (MMP) likely relates to placenta release. So we tried to search for the activator of placental MMP using cultured bovine fibroblast derived from placenta. First, we forcused on the metabolites of arachidonic acid (Ara) because those play important roles at the process of delivery. Cyclooxygenase metabolites of Ara (prostanoid) had no activity on MMP activation; however, one of lipoxygenase metabolites showed a strong activity on MMP activation. Delivery induction using prostaglandin (PG) frequently results in retained placenta. However, we succeeded in placenta release of those cows by injection of lipoxygenase metabolite of Ara. And the zymographic pattern of MMP activity of placenta in this case was same as that in normal delivery. While a large amount of proMMP (inactive form) accumulation in placenta was observed in the cases of PG-induced delivery and naturally occoured retained placenta, which suggested the failure of MMP activation. These results indicated that lipoxygenase metabolite of Ara is a signal for MMP activation and placenta release at a delivery. We could develope a new method of delivery induction without retained placenta.
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Report
(5 results)
Research Products
(70 results)
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[Journal Article] H. Kamada, I. Nonaka, Y. Ueda M. Murai2006
Author(s)
H. Kamada
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Journal Title
Seleniu mincreases immunoglobulin G absorption by the int estinal pinocytosis of newborn calves. Book of Abstracts of the 57th Annual Meeting of the European Association for Animal Production
Pages: 120-120
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Francois, Biochemical and transcriptomic analyses of two bovine skeletal muscles in Charolasis bulls divergently selected for muscle growth.2005
Author(s)
K. Sudre, I. Cassari-Malek, A Listrat, Y. Ueda, C. Leroux, C. Jurie, C. Auffray, G. Renand, P. Martin, J.
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Journal Title
Meat Science
Pages: 267-277
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] 「研究成果報告書概要(欧文)」より2005
Author(s)
I. Cassari-Malek, Y. Ueda, C. Bernard, C. Jurie, K. Sudre, A. Listrat, I. Barnola, G. Gentes, C. Leroux, G. Renand, P. Martin, J. F. Hocquette
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Journal Title
Indicators of milk and beef quality
Pages: 371-377
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[Presentation] 泌乳牛の放牧時間と乳中香気成分との関係2008
Author(s)
上田靖子, 朝隈貞樹, 秋山典昭, 宮地慎, 村井勝, 松村哲夫, 篠田満
Organizer
第109回日本畜産学会
Place of Presentation
常磐大学
Year and Date
2008-03-29
Description
「研究成果報告書概要(和文)」より
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[Book] 臨床獣医2007
Author(s)
鎌田八郎
Total Pages
4
Publisher
緑書房
Description
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[Book] 肥料2005
Author(s)
鎌田八郎
Total Pages
4
Publisher
肥料協会新聞部
Description
「研究成果報告書概要(和文)」より
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[Book] Indicators of milk and beef quality2005
Author(s)
I. Cassari-Malek, Y. Ueda, C. Bernard, C. Jurie, K. Sudre, A. Listrat, I. Barnola, G. Gentes, C. Leroux, G. Renand, P. Martin, J. F. Hocquette
Total Pages
6
Publisher
Wageningen Academic Publishers
Description
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