Visualization of spatiotemporal activation of Notch signaling : live monitoring and significance in neural development and Hematopoiesis
| Project/Area Number |
16390054
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Keio University |
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Principal Investigator |
MATSUZAKI Yumi Keio University, School of Medicine, Associate Professor, 医学部, 助教授 (50338183)
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| Co-Investigator(Kenkyū-buntansha) |
SAWAMOTO Kazuhobu Keio University, School of Medicine, Associate Professor, 医学部, 助教授 (90282350)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2006: ¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2005: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2004: ¥5,400,000 (Direct Cost: ¥5,400,000)
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| Keywords | Notch / Stem cells / Self-renew / Transgenic / Wnt / トランスジェニックマウス / 造血幹細胞 / 神経幹細胞 / レポーター遺伝子 / Notchシグナル / Wntシグナル / 自己複製能 |
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| Research Abstract |
Notch signaling plays various key roles in cell fate determination during CNS development in a context-dependent fashion. However, its precise physiological role and the localization of its target cells remain unclear. To address iris issue, we developed a new reporter system for assessing the RBP-J-mediated activation of Notch signaling target genes in living cells and tissues using a fluorescent protein Venus. Our reporter system revealed. that Notch signaling is selectively activated in neurosphere-initiating multipotent neural stem cells in vitro and in radial glia in the embryonic forebrain in vivo. Furthermore, the activation of Notch signaling occurs during gliogenesis and is required in the early stage of astroglial development. Consistent with these Endings, the persistent activation of Notch signaling inhibits the differentiation of GFAP-positive astrocytes. Thus, the development of our RBP-J-dependent live reporter system, which is activated upon Notch activation, together with a stage-dependent gain-of-function analysis allowed us to gain further insight into the complexity of Notch signaling in mammalian CNS development.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] GFP transgenic mice reveal active canonical Wnt signal in neonatal brain and in 'adult liver and spleen.2007
Author(s)
Moriyama A, Kii I, Sunabori T, Kurihara S, Takayama I, Shimazaki M, Tanabe H, Oginuma M, Fukayama M, Matsuzaki Y, Saga Y, Kudo A
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Journal Title
Genesis 45(2)
Pages: 90-100
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cardiac neural crest cells contribute to the dormant multipotent stem cell in the mammalian heart.2005
Author(s)
Tomita Y, Matsumura K, Wakamatsu Y, Matsuzaki Y, Shibuya I, Kawaguchi H, Ieda M, Kanakubo S, Shimazaki T, Ogawa S, Osumi N, Okano H, Fukuda K
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Journal Title
Journal of Cell Biology 170(7)
Pages: 1135-1146
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[Journal Article] Leukemia inhibitory factor induces multi-lineage differentiation of adult stem-like cells in kidney via kidney-specific cadherin 162005
Author(s)
Hishikawa K, Marumo T, Miura S, Nakanishi A, Matsuzaki Y, Shibata K, Kohike H, Komori T, Hayashi M, Nakaki T, Nakauchi. H, Okano H, Fujita T
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Journal Title
Biochemical and Biophysical Research Communications 328(1)
Pages: 288-291
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[Journal Article] Musculin/MyoR is expressed in kidney side population cells and can regulate their function2005
Author(s)
Hishikawa K, Marumo T, Miura S, Nakanishi A, Matsuzaki Y, Shibata K, Ichiyanagi T, Kohike H, Komori T, Takahashi I, Takase O, Imai N, Yoshikawa M, Inowa T, Hayashi M, Nakaki T, Nakauchi H, Okano H, Fujita T
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Journal Title
Journal of Cell Biology 169(6)
Pages: 921-928
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[Book] 医学のあゆみ2006
Author(s)
松崎有未
Total Pages
232
Publisher
医歯薬出版
Description
「研究成果報告書概要(和文)」より
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