• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study on effects and transport of nanoparticles through alveolar wall

Research Project

Project/Area Number 16390186
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Public health/Health science
Research InstitutionNational Institute for Environmental Studies

Principal Investigator

FURUYAMA Akiko  National Institute for Environmental Studies, PM2.5&DEP Research Project, Senior Researcher, M2・5・DEP研究プロジェクト, 主任研究員 (20177661)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥9,200,000 (Direct Cost: ¥9,200,000)
Fiscal Year 2005: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 2004: ¥4,100,000 (Direct Cost: ¥4,100,000)
KeywordsNano Darticle / Inhalation toxicology / alveolo-capillarv barrier model / translocation / alveolar wall
Research Abstract

We have prepared an in vitro alveolo-capillary barrier model using alveolar epithelial and endothelial cells to evaluate the behavior of nanoparticles in the respiratory system. Colloidal gold or fluorescent-tagged polystyrene particles of 200 nm and 20 nm were taken up by the alveolar epithelial cells by phagocytosis or endocytosis. Twe hundred nm particles were present only in the alveolar epithelial cells and 20 nm particles were found in the endothelial cells, suggesting that 20 nm but not 200nm particles have an ability to penetrate the alveolo-capillary barrier model. Multi-wall and single-wall carbon nanotubes and 200nm polystyrene particles induced hemeoxygenase-1 in alveolar epithelial cells but 20 nm particles did not. Secretion of cytokines (IL-1β and TNF-α) or activation of NF-κB were not observed. Activation of MAP kinase was observed in cells exposed to carbon nanotubes.
To investigate the behavior of nanoparticles deposited in the respiratory airways, we have also complet … More ed an intratracheal instillation study using colloidal gold particles and fluorescent-tagged polystyrene particles (20 nm, 200nm, FluoSphere【○!R】carboxylated-modifiedmicrospheres, red fluorescent 580/605). Most of the particles were found in alveolar macrophages. It is of interest to note that the 20nm particles were present in the microvessel, while the aggregated 20nm and 200nm particles that had been engulfed by alveolar macrophages and alveolar epithelial cells. Alveolar macrophages containing particles were observed in the cavity of heart, the vessel of liver and kidney. ICP-MS analysis revealed that a little amount of gold was detected in blood, heart, liver, kidney, spleen, lymph node after 2 hr instillation.
To investigate the effect of nanoparticles deposited in the respiratory airways, we have also completed an intratracheal instillation study using single and maulti-wall carbon nanotubes, fullerene, titanium oxide. Activation of NF-κB were not observed, but upregulation of cytokine secretion (IL-1β, TNF-α, and TGF-β) was detected by instillation of single and multi-wall carbon nanotubes (10 and 50μg/mouse). Instillation of carbon nanotubes induced inflammation, granulomas, and weak fibrosis in lung.
These results suggest that nanoparticle of 20 nm may penetrate the alveolar wall (epithelial cells, basement membrane, and endothelial cells) and has a potency to migrate directly into the circulation, although it requires an additional study using electron microscopy to confirm the "Direct migration" scenario. Less

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (6 results)

All 2006 2005

All Journal Article (4 results) Book (2 results)

  • [Journal Article] Induction of oxidative stress and inhibition of plasminogen activator inhibitor-1 production in endothelial cellsfollowing exposure to organic extracts of diesel exhaust particles and urban fine particles.2006

    • Author(s)
      Furuyama, A. et al.
    • Journal Title

      Arch Toxicol 80(3)

      Pages: 154-162

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Induction of oxidative stress and inhibition of plasminogen activator inhibitor -1 production in endothelial cells following exposure to organic extracts of diesel exhaust particles and urban fine particles.2006

    • Author(s)
      Furuyama, A. et al.
    • Journal Title

      Arch Toxicol. 80(3)

      Pages: 154-162

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Deposition and translocation of nanoparticles.2005

    • Author(s)
      Furuyama, A.
    • Journal Title

      The measurement and influence of automobile discharged nanoparticles and DEP. (Ohsei, Y. et al.,)(NTS Co. ltd., Tokyo)(239P)

      Pages: 159-164

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Induction of oxidative stress and inhibition of plasminogen activator inhibitor-1 production in endothelial cells following exposure to organic extracts of diesle exhaust particles and urban fine particles.2005

    • Author(s)
      Furuyama, A. et al.
    • Journal Title

      Arch Toxicol. 80(3)

      Pages: 154-162

    • Related Report
      2005 Annual Research Report
  • [Book] 自動車排出ナノ粒子およびDEOの測定と生体影響2005

    • Author(s)
      大聖泰弘他
    • Total Pages
      239
    • Publisher
      株式會社エヌ・ティー・エス
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 自動車排出ナノ粒子およびDEPの測定と生体影響2005

    • Author(s)
      大聖泰弘, 他
    • Total Pages
      239
    • Publisher
      株式会社エヌ・ティー・エス
    • Related Report
      2005 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi