Visualization of insulin granules in pancreatic beta cells derived from genetically altered mouse models.
| Project/Area Number |
16390261
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Gunma University |
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Principal Investigator |
IZUMI Testuro Gunma University, Department of Molecular medicine, Institute for Molecular and Cellular Regulation, Professor, 生体調節研究所, 教授 (00212952)
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| Co-Investigator(Kenkyū-buntansha) |
GOMI Hiroshi Gunma University, Department of Molecular medicine, Institute for Molecular and Cellular Regulation, Associated professor, 生体調節研究所, 助教授 (90293240)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥13,800,000 (Direct Cost: ¥13,800,000)
Fiscal Year 2005: ¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 2004: ¥8,000,000 (Direct Cost: ¥8,000,000)
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| Keywords | Insulin / Secretory granules / Regulated exocytosis / Fluorescent protein / Mutated mouse |
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| Research Abstract |
We investigated the exocytosis of insulin granules in Rab27a-mutated ashen mice and granuphilin-knockout mice. We found that both Rab27a and its effector granuphilin are involved in the docking of insulin granules onto the plasma membrane. We then visualized insulin granules by expressing green fluorescent protein (GFP)-fused phogrin in a beta cell-derived cultured cell line. When granuphilin was overexpressed in the cell, the visualized granules were redistributed to the peripheral area close to the plasma membrane. We also tried to monitor the fusion of insulin granules by expressing insulin fused with two different fluorescent proteins, Ins-C-Timer and Ins-GFP. We found that the latter protein is more suitable to monitor the fusion and currently perform the imaging analyses in ashen or granuphilin-null beta cells.
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Report
(3 results)
Research Products
(15 results)
