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Establishment of therapeutic strategy for degenerative muscular disease by mouse or human somatic stem cells

Research Project

Project/Area Number 16390300
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionKyoto University

Principal Investigator

HEIKE Toshio  Kyoto University, Graduate School of Medicine, Associate Professor, 医学研究科, 助教授 (90190173)

Co-Investigator(Kenkyū-buntansha) YORIFUJI Tohru  Kyoto University, Graduate School of Medicine, Lecturer, 医学研究科, 講師 (60220779)
HIRAMATSU Hidefumi  Kyoto University, Graduate School of Medicine, Assistant professor, 医学研究科, 助手 (40362503)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2005: ¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 2004: ¥7,400,000 (Direct Cost: ¥7,400,000)
Keywordsskeletal muscle / hematopoietic stem cell / GFP / c-kit / Sca-1 / Lin / satellite cell / muscular stem cell / 多能性幹細胞 / sphere / 筋ジストロフィー症 / 間葉系幹細胞 / 再生 / 機能
Research Abstract

The contribution of hematopoietic stem cells into the regeneration of skeletal muscles was investigated. The regenerative pathway of mouse skeletal muscles after the transplantation of GFP-transgenic mouse-derived c-kit+ Sca-1+ Lin- cells along with irradiation was observed precisely. The GFP-positive skeletal muscles was detected 1 week after transplantation. This GFP-positive muscles were replaced by GFP-negative ones gradually and could not be detected thereafter. At 6 months after transplantation, GFP-positive muscles could not be confirmed. However, surprisingly, single muscle fiber culture at 6 months revealed that GFP-positive muscle fibers could be produced and GFP-positive satellite cells (skeletal muscle stem cells) was demonstrated along muscle fibers. These results imply that c-kit+ Sca-1+ Lin- cells contribute to the regeneration of skeletal muscle at not only short terms but also long terms.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (21 results)

All 2005 2004 Other

All Journal Article (21 results)

  • [Journal Article] Stem cell plasticity in hematopoietic system2005

    • Author(s)
      Heike T
    • Journal Title

      Inflammation and Regeneration 25

      Pages: 90-101

    • NAID

      10011958317

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Two different roles of purified CD45+ c-kit+Sca-1+Lin- cells after transplantation in muscles2005

    • Author(s)
      Yoshimoto M
    • Journal Title

      Stem Cells 23

      Pages: 610-618

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Prospective characterization of neural stem cells by flow cytometry anakysis using a combination of surface2005

    • Author(s)
      Nagato M
    • Journal Title

      J Neurosci Res 80

      Pages: 456-466

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Identification of cardiac stem cells withFLK1, CD31, and VE-cadherin expression during embryonic stem cell differentiation2005

    • Author(s)
      Iida M
    • Journal Title

      FASEB J 19

      Pages: 371-378

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Stem cell plasticity in hematopoietic system2005

    • Author(s)
      Heike T., Nakahata T.
    • Journal Title

      Inflammation and Regeneration 25

      Pages: 90-101

    • NAID

      10011958317

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Two different roles of purified CD45+ c-kit+ Sca-1+ Lin- cells after transplantation in muscles.2005

    • Author(s)
      Yoshimoto M., Chang H., Shiota M., Kobayashi H., Umeda K., Kawakami A., Heike T., Nakahata T.
    • Journal Title

      Stem Cells 23

      Pages: 610-618

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Prospective characterization of neural stem cells by flow cytometry anakysis using a combination of surface markers.2005

    • Author(s)
      Nagato M., Heike T., Kato T., Yamanaka Y., Yoshimoto M., Shimazaki T., Okano H., Nakahata T.
    • Journal Title

      J Neurosci Res 80

      Pages: 456-466

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Identification of cardiac stem cells with FLK1, CD31, and VE-cadherin expression during embryonic stem cell differentiation.2005

    • Author(s)
      Iida M., Heike T., Yoshimoto M., Baba S., Doi H., Nakahata T.
    • Journal Title

      FASEB J. 19

      Pages: 371-378

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Stem cell plasticity in hematopoietic system2005

    • Author(s)
      Heike T
    • Journal Title

      Inflamation and Regeneration 25

      Pages: 90-90

    • NAID

      10011958317

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Two different roles of purified CD45+ c-kit+ Sca-1+ Lin- cells after transplantation in muscles2005

    • Author(s)
      Yoshimoto M
    • Journal Title

      Stem Cells 23

      Pages: 610-610

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Identification of cardiac stem cells with FLK1, CD31, and VE-cadherin expression during embryonic stem cell differentiation2005

    • Author(s)
      Iida M
    • Journal Title

      FASEB J 19

      Pages: 371-371

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Generation of pluripotent stem cells from neonatal mouse testis2004

    • Author(s)
      Kanatsu-Shinohara M
    • Journal Title

      Cell 119

      Pages: 1001-1012

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Generation of pluripotent stem cells from neonatal mouse testis.2004

    • Author(s)
      Kanatsu-Shinohara M., Inoue K., Lee J., Yoshimoto M., Ogonuki N., Miki H., Baba S., Kato T., Kazuki Y., Toyokuni S., Toyoshima M., Niwa O., Oshimura M., Heike T., Nakahata T., Ishino F., Ogura A., Shinohara T.
    • Journal Title

      Cell 119

      Pages: 1001-1012

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Development of both human connective tissue-type and mucosal-type mast cells in mice from hematopoietic stem cells with identical distribution pattern to human body2004

    • Author(s)
      Kambe N, Heike T et al.
    • Journal Title

      Blood 103

      Pages: 860-867

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Stem cell plasticity in hematopoietic system2004

    • Author(s)
      Heike T, Nakahata T
    • Journal Title

      Int J Hematol 79

      Pages: 7-14

    • NAID

      10011958317

    • Related Report
      2004 Annual Research Report
  • [Journal Article] X-linked ectodermal dysplasia and immunodeficiency caused by reversion mosaicism of NEMO reveals a critical role for NEMO in human T-cell development and/or survival2004

    • Author(s)
      Nishikomori R, Heike T et al.
    • Journal Title

      Blood 103

      Pages: 4565-4572

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Development of primitive and definitive hematopoiesis from nonhuman primate embryonic ste cells in vitro2004

    • Author(s)
      Umeda K, Heike T et al.
    • Journal Title

      Development 131

      Pages: 1869-1879

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Differential requirement of the CD40-CD154 costimulatory pathway during T helper cell proming by CD8alpha+ and CD8alpha- murine dendritic cell subsets2004

    • Author(s)
      Yasumi T, Heike T et al.
    • Journal Title

      J Immunol 172

      Pages: 4826-4833

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Generation of pluripotent stem cells from neonatal mouse testis2004

    • Author(s)
      Nakatsu-Shinohara M, Heike T et al.
    • Journal Title

      Cell 119

      Pages: 1001-1012

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A neurosphere-derived factor, Cystatin C, supports differentiation of ES cells into neural stem cells

    • Author(s)
      Kato T
    • Journal Title

      Proc.Natl.Acad.Sci.USA (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Identification and characterization of hemangiogenic progenitors during cynomolous monkey embryonic stem cell differentiation

    • Author(s)
      Umeda K
    • Journal Title

      Stem Cells (in press)

    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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