Genethraphy for the patients with metastatic or refractory osteosatcoma using Ad-OC-TK gene
| Project/Area Number |
16390440
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kobe University |
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Principal Investigator |
AKISUE Toshihiro (2005-2007) Kobe University, Graduate School of Medicine, Assistant Professor (90379363)
山本 哲司 (2004) 神戸大学, 大学院・医学系研究科, 講師 (80220482)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIDA Kotaro Kobe University, Graduate School of Medicine, Assistant Professor (00379372)
GOTO Akinobu Hyogo College of Medicine, 医学部, Professor (70283885)
SHIRAKAWA Toshiro Kobe University, Graduate School of Medicine, Associate Professor (70335446)
藤本 卓也 神戸大学, 医学系研究科, 医学研究員 (00397811)
秋末 敏宏 神戸大学, 大学院・医学系研究科, 助手 (90379363)
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| Project Period (FY) |
2004 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥13,810,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2004: ¥5,300,000 (Direct Cost: ¥5,300,000)
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| Keywords | Genetheraphy / Adenovirus / Osteocalcin / Clinical Trial / Gene Expression / Osteosarcoma / 悪性腫瘍 / 骨肉種 |
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| Research Abstract |
We have established TNMY cell lines from the retrieved MFH. We tested the effects of PTK787 (inhibitor for VEGF receptors) on cell proliferation, invasion, angiogenesis of Nara H, Nara F, GBS-1, and TNMY.) We have not found the inhibitory effect of PTK787 on cell proliferation of MFH cell lines. Cell invasion of Nara H cells was inhibited by PTK787. All four cell lines showed inhibitory effects of PTK787 on angiogenesis. In the study of DcR3 decoy receptors related to apoptosis, inhibitory effect of siRNA on mRNA expression of DcR3 could be detected in osteosarcoma and MFH cell lines. The extent of protein expression in MFH cells was higher than that in osteosarcoma cells after siRNA treatment for DcR3. In osteosarcoma cells, the number of TUNEL positive cells after siRNA+FasL treatment was same as that after contral siRNA+FasL treatment, but the number of TUNEL positive cells after siRNA+FasL+adriamycin treatment was higher than that after contral siRNA+FasL+adriamycin treatment, indicating that siRNA+FasL+adriamycin treatment induced apoptosis in osteosarcoma cells. In the study of Sphingosine-1-posphate (S1P) and EDG receptors for S1P in sarcomas, we found that mRNA expression of EDG1, 3, and 5 in high-grade sarcomas was higher than that in low-grade sarcomas. Expression of Sphingosine kinase 2 (SPHK2) in low-grade sarcomas was higher that that in high-grade sarcomas, whereas we did not find the difference in mRNA expression of SPHK1 between low-grade and high-grade sarcomas
We produced Ad-OC-TK gene probes for clinical application at CPC cell culture center in Gene Medicine Japan and we tested the quality and security of probes for clinical use. The results of the tests were excellent for clinical use of Ad-OC-TK gene probes.
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Report
(5 results)
Research Products
(22 results)
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[Journal Article] High level of ezrin mRNA expression in an osteosarcoma biopsy sample with lung metastasis.2007
Author(s)
Ogino, W, Takeshima, Y, Mori, T, Yanai, T, Hayakawa, A, Akisue, T, et. al.
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Journal Title
J Pediatr Hematol Onco 29
Pages: 435-439
Description
「研究成果報告書概要(欧文)」より
Related Report
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