DISC REPAIR WITH HUMAN CELL, THERAPYINFLUENCED BY MESENCHYMAL STEM CELLS
| Project/Area Number |
16390443
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokai University |
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Principal Investigator |
MOCHIDA Joji Tokai University, SCHOOL OF MEDICINE, PROFESSOR (50174347)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAI Daisuke TOKAI UNIVERSITY, SCHOOL OF MEDICINE, FELLOW (10408007)
NOMURA Takeshi TOKAI UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR (60246121)
OKUMA Masahiko TOKAI UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR (10317787)
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| Project Period (FY) |
2004 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥15,250,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2007: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2006: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥3,700,000 (Direct Cost: ¥3,700,000)
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| Keywords | cell transplantation therapy / regenerative medicine / disc regeneration / nucleus pulposus cell / mesenchymal stem cell / 骨髄間葉系幹細胞 / 共培養法 / 椎間板変性 / 臨床応用 |
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| Research Abstract |
Since only a very small number of nucleus pulposus cells could be obtained and their biological activity was low, it is necessary to develop a new activation method or find a new source of cells to replace the degenerated NP cells. Utilizing animal models, the data on the number of cells and the synthesis of DNA and proteoglycans showed that the nucleus pulposus cells were effectively activated by coculture system with bone marrow-derived mesenchymal stem cell having direct cell-to-cell contact. In vivo study using animal models such as beagle dog with disc degeneration also revealed to decelerate the velocity of progression of disc degeneration or regeneration. For the human application of the activated nucleus pulposus cells, an activation of human nucleus pulposus cells by human mesenchymal stem cells with coculture having direct cell-to-cell contact was also evaluated. The synthesis of DNA and proteoglycan revealed 5- fold higher than in the monolayer culture. No abnormality on the chromosome was revealed. Tumor genesis was not observed. Therefore, we concluded that the activated nucleus pulposus cells are very useful and safe for clinical application.
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Report
(5 results)
Research Products
(70 results)
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[Journal Article] Atelocollagen for culture of human nucleus pulposus cells forming nucleus pulposus-like tissue in vitro : influence on the proliferation and proteoglycan production of HNPSV-1 cells2006
Author(s)
Sakai, D, Mochida, J, Iwashina, T, Watanabe, T, Suyama, K, Ando, K, Hotta, T
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Journal Title
Biomaterials 27
Pages: 346-353
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Regenerative effects of transplanting mesenchymal stem cells embedded in atelocollagen to the degenerated intervertebral disc2006
Author(s)
Sakai, D, Mochida, J, Iwashina, T, Hiyama, A, Omi, H, Imai, M, Nakai, T, Ando, K, Hotta, T
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Journal Title
Biomaterials 27
Pages: 335-345
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Low-intensity pulsed ultrasound stimulates cell proliferation and proteoglycan production in rabbit intervertebral disc cells cultured in alginate2006
Author(s)
Iwashina, T, Mochida, J, Miyazaki, T, Watanabe, T, Iwabuchi, S, Ando, K, Hotta, T, Sakai, D
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Journal Title
Biomaterials 27
Pages: 354-361
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Differentiation of mesenchymal stem cells transplanted to a rabbit degenerative disc model. potential and limitation for stem cell therapy in disc degeneration2005
Author(s)
Sakai, D, Mochida, J, Iwashina, T, Watanabe, T, Nakai, T, Ando, K, Hotta, T
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Journal Title
Spine 30
Pages: 2379-2387
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Upregulation of the viability of nucleus pulposus cells by bone marrow-derived stromal cells ; significance of direct cell-to-cell contact in coculture system2004
Author(s)
Yamamoto, Y, Mochida, J, Sakai, D, Nakai, T, Nishimura, K, Kawada, H, Hotta, T
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Journal Title
Spine 29
Pages: 1508-1514
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Immortalization of human nucleus pulposus cells by recombinant SV 40 adenovirus vector : Establishment of a novel cell line for the study of human nucleus pulposus cells2004
Author(s)
Sakai, D, Mochida, J, Yamamoto, Y, Toh, E, Iwashina, T, Miyazaki, T, Inokuchi, S, Ando, K, Hotta, T
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Journal Title
Spine 29
Pages: 1515-1523
NAID
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 椎間板再生への細胞移植療法の展開2007
Author(s)
持田 譲治, 酒井 大輔, 岩品 徹, 檜山 明彦, 大見 博子, 芹ケ野 健司
Organizer
第80回日本整形外科学会学術総会
Place of Presentation
神戸市
Description
「研究成果報告書概要(和文)」より
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