Molecular biological analysis of mechanisms for human embryo implantation and placentation-from the perspectrives of repulsive force, chemoattractants and membrane-bound peptidases
| Project/Area Number |
16390474
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
FUJIWARA Hiroshi Kyoto University, Graduate school of Medicine, Lecturer, 医学研究科, 講師 (30252456)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIOKA Shinya Kyoto University, Graduate school of Medicine, Assistant, 医学研究科, 助手 (90333575)
HIGUCHI Toshihiro Kyoto University, Graduate school of Medicine, Assistant, 医学研究科, 助手 (00283614)
FUJITA Jun Kyoto University, Graduate school of Medicine, Prof., 医学研究科, 教授 (50173430)
NISHI Eiichirou Kyoto University, Graduate school of Medicine, Associate Prof., 医学研究科, 助教授 (30362528)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2005: ¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 2004: ¥8,200,000 (Direct Cost: ¥8,200,000)
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| Keywords | Endometrium / Eph-ephrin / Embryo / Extravillous trophoblast / Membrane-bound peptidase / 細胞膜結合型peptidase |
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| Research Abstract |
To confirm the physiological roles of repulsive force-inducing factors in the regulation of human embryo attachment to endometrium and penetration through endometrial epithelial layer, we examined the expression profiles of Eph-ephrin A molecules and neuropilin-semaphorin molecules in the human endometrium and embryo. As a result, we found Eph A1, A2, A4 and ephrin A1 were expressed on human endometrial epithelial cells, while human and murine embryo expressed ephrin A1 and Eph A1. Attachment assay revealed that immobilized Eph A1 reduced murine embryo attachment, showing the possibility that Eph-ephrin A system regulates the timing of cross-talk period between maternal endometrium and embryo before attachment to endometrium.
On the other hand, by cell aggregation assay, ephrin A1 signal promoted cell-dissociation among endometrial carcinoma-derived cell line, Ishikawa cells. This reaction was reversible and induced tyrosine phosphorylation of β-catenin. These findings suggest that Eph-ephrin system can induce intercellular dissociation among the endometrial epithelial cell layer.
The subsequent analysis showed that extravillous trophoblast invasion was induced by chemokines and chemokine-degrading peptidases. It was also indicated that platelets that were deposited in the maternal spiral arteries play an important role in inducing extravillous trophoblast invasion into the artery by secreting chemoattractive substances, creating chemokine gradient around spiral artery.
These findings showed that repulsive and chemoattractive forces are involved in early embryo implantation and placentation.
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Report
(3 results)
Research Products
(15 results)
